Study on role of ATP in Watasenia scintillans bioluminescence

• Project/Area Number: 22603005
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: ケミカルバイオロジー
• Research Institution: Mie University
• Principal Investigator: TERANISHI Katsunori 三重大学, 大学院・生物資源学研究科, 教授 (20237001)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 発光 / ATP

Research Abstract

In a study on role of ATP in the bioluminescence of Watasenia scintillance, 1) a method of purification of luminescence enzyme has been developed, 2) it was shown that the amount of ADP production in the luminescence reaction is lower than that of luminescence product, 3) it was shown that ATP equilibriumly binds and dissociate without covalent binding, 4) water-insoluble luminescence enzyme is active in the solid state.