The relationship between actin bundling by dynamin/cortactin complex and stretch-activated cation channel on actin cytoskeleton
| Project/Area Number |
22616004
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Mechanobiology
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| Research Institution | Okayama University |
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Principal Investigator |
YAMADA Hiroshi 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (80325092)
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| Co-Investigator(Kenkyū-buntansha) |
片野坂 友紀 岡山大学, 大学院・医歯薬学総合研究科, 助教 (60432639)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | アクチン / ダイナミン / 機械受容チャネル / コルタクチン |
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| Research Abstract |
The function of stretch-activated cation channel on actin dynamics was investigated. Calcium ion concentration in the ruffle membrane was increased during cell migration. Knockdown of the channel in cancer cell line resulted in marked reduction ofboth calcium influx via the channel and serum-stimulated ruffle formation, which were required for cell migration. These results suggest that stretch-activated cation channel in cancer cell is involved in cell migration possibly by regulating actin dynamics. Furthermore, we identified the function of dynamin/cortactin complex in the regulation of actin dynamics. The dynamin/cortactin complexes bundled several actin filaments and the bundling stabilized actin filaments. The actin bundling by dynamin/cortactin complex was necessary for formation of growth cone filopodia and cell migration. These findings might be crucial for the study for neuronal regeneration and cancer cell migration.
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Report
(4 results)
Research Products
(29 results)
