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The relationship between actin bundling by dynamin/cortactin complex and stretch-activated cation channel on actin cytoskeleton

Research Project

Project/Area Number 22616004
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Mechanobiology
Research InstitutionOkayama University

Principal Investigator

YAMADA Hiroshi  岡山大学, 大学院・医歯薬学総合研究科, 准教授 (80325092)

Co-Investigator(Kenkyū-buntansha) 片野坂 友紀  岡山大学, 大学院・医歯薬学総合研究科, 助教 (60432639)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsアクチン / ダイナミン / 機械受容チャネル / コルタクチン
Research Abstract

The function of stretch-activated cation channel on actin dynamics was investigated. Calcium ion concentration in the ruffle membrane was increased during cell migration. Knockdown of the channel in cancer cell line resulted in marked reduction ofboth calcium influx via the channel and serum-stimulated ruffle formation, which were required for cell migration. These results suggest that stretch-activated cation channel in cancer cell is involved in cell migration possibly by regulating actin dynamics. Furthermore, we identified the function of dynamin/cortactin complex in the regulation of actin dynamics. The dynamin/cortactin complexes bundled several actin filaments and the bundling stabilized actin filaments. The actin bundling by dynamin/cortactin complex was necessary for formation of growth cone filopodia and cell migration. These findings might be crucial for the study for neuronal regeneration and cancer cell migration.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (29 results)

All 2013 2012 2011 2010 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (16 results) Book (1 results) Remarks (2 results) Patent(Industrial Property Rights) (4 results)

  • [Journal Article] Stabilization of actin bundles by a Dynamin 12013

    • Author(s)
      Yamada H, Abe T
    • Journal Title

      Cortactin ring complex is necessary for growth cone filopodia.

      Volume: 33 Issue: 10 Pages: 4514-4526

    • DOI

      10.1523/jneurosci.2762-12.2013

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel gene expression system for detecting viable bladder cancer cells2012

    • Author(s)
      Hideo Ueki, et al
    • Journal Title

      International journal of oncology

      Volume: 41 Pages: 135-140

    • DOI

      10.3892/ijo.2012.1417

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] REIC/Dkk-3-encoding adenoviral vector as a potentially effective therapeutic agent for bladder cancer.2012

    • Author(s)
      Hirata et al.
    • Journal Title

      Int J Oncol.

      Volume: 41 Issue: 2 Pages: 559-564

    • DOI

      10.3892/ijo.2012.1503

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development2010

    • Author(s)
      Zhang, K.,Watanabe, M, (他17人)
    • Journal Title

      Int. J. Oncol

      Volume: 37 Issue: 6 Pages: 1495-1501

    • DOI

      10.3892/ijo_00000802

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development.2010

    • Author(s)
      Kai Zhang
    • Journal Title

      International Journal of Oncology

      Volume: 37 Pages: 1495-1501

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Use of liposomes to study vesicular transport.2010

    • Author(s)
      竹居孝二
    • Journal Title

      Methods in Molecular Biology

      Volume: 606 Pages: 531-542

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Dynamin GTPase / Cortactin ring complex is a mechanochemical device stabilizing actin bundles2012

    • Author(s)
      竹居 孝二
    • Organizer
      日本生体エネルギー研究会 第38回討論会
    • Place of Presentation
      岡山大学薬学部 (岡山)
    • Year and Date
      2012-12-23
    • Related Report
      2012 Final Research Report
  • [Presentation] Dynamin を標的にした抗遊走、浸潤薬の開発2012

    • Author(s)
      岡崎 奈奈
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡(福岡)
    • Year and Date
      2012-12-16
    • Related Report
      2012 Final Research Report
  • [Presentation] Phosphorylation of cortactin by PKC is a key factor for filopodia formation of growth cone2012

    • Author(s)
      山田 浩司
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡(福岡)
    • Year and Date
      2012-12-15
    • Related Report
      2012 Final Research Report
  • [Presentation] PKC phosphorylation of cortactin is implicated in the regulationof actin dynamics2011

    • Author(s)
      竹居 孝二
    • Organizer
      第51回米国細胞生物学会大会
    • Place of Presentation
      デンバー(米国)
    • Year and Date
      2011-12-04
    • Related Report
      2012 Final Research Report
  • [Presentation] PKC phosphorylation of oortactin is implicated in the regulation of actin dynamics2011

    • Author(s)
      竹居孝二
    • Organizer
      第51回米国細胞生物学会大会
    • Place of Presentation
      デンバー:米国
    • Year and Date
      2011-12-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] 成長円錐糸状仮足形成におけるCortactinのPKCαによるリン酸化とアクチン制御の可能性2011

    • Author(s)
      山田 浩司
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2011-09-22
    • Related Report
      2012 Final Research Report
  • [Presentation] 成長円錐糸状仮足形成におけるCortactinのPKCαこよるリン酸化とアクチン制御の可能性2011

    • Author(s)
      山田浩司
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] Actin bundle formation by dynamin-cortactin complexes is important for filopodium stability of growth cones2011

    • Author(s)
      山田 浩司
    • Organizer
      日本薬学会第131年会
    • Year and Date
      2011-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] Actin bundle formation by dynamin-cortactin complexes is important for filopodium stability of growth cones.2011

    • Author(s)
      山田浩司
    • Organizer
      日本薬学会
    • Place of Presentation
      静岡
    • Year and Date
      2011-03-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 、Dynamin 1/Cortactin complex mechanically bundles actin filaments and supports the formation of filopodia.2010

    • Author(s)
      竹居 孝二
    • Organizer
      第33回日本神経科学、第53回日本神経化学、第20回日本神経回路学会合同年会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2010-09-03
    • Related Report
      2012 Final Research Report
  • [Presentation] Dynamin 1/Cortactin complex mechanically bundles actin filaments and supports the formation of filopodia.2010

    • Author(s)
      竹居孝二
    • Organizer
      第33回日本神経科学、第53回日本神経化学、第20回日本神経回路学会合同年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-09-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] Amphiphysin 1 とN-WASPの動的な相互作用はアクチン重合を制御する2010

    • Author(s)
      山田 浩司
    • Organizer
      第33回日本神経科学、第53回日本神経化学、第20回日本神経回路学会合同年会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2010-09-02
    • Related Report
      2012 Final Research Report
  • [Presentation] Amphiphysin 1とN-WASPの動的な相互作用はアクチン重合を制御する2010

    • Author(s)
      山田浩司
    • Organizer
      第33回日本神経科学、第53回日本神経化学、第20回日本神経回路学会合同年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-09-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] Phosphorylation of cortactin by PKC is a key factor for filopodia formation of growth cone

    • Author(s)
      山田 浩司
    • Organizer
      第85回生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] Dynaminを標的にした抗遊走、浸潤薬の開発

    • Author(s)
      岡﨑 奈奈
    • Organizer
      第85回生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] Dynamin GTPase / Cortactin ring complex is a mechanochemical device stabilizing actin bundles

    • Author(s)
      竹居 孝二
    • Organizer
      日本生体エネルギー研究会 第38回討論会
    • Place of Presentation
      岡山大学薬学部
    • Related Report
      2012 Annual Research Report
  • [Book] Use of liposomes to study vesicular transport.2010

    • Author(s)
      Takei K, Yamada H, Abe T
    • Publisher
      Methods in Molecular Biology
    • Related Report
      2012 Final Research Report
  • [Remarks] (1)「神経伸長制御の仕組みを解明」 山陽新聞、平成25年3月30日

    • Related Report
      2012 Final Research Report
  • [Remarks] (2)「神経細胞をつなげる2種のたんぱく質」読売新聞、平成25年4月8日

    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] フルボキサミンを配合することを特徴とする分子標的型抗癌剤(特に抗脳腫瘍治療薬剤:第二医薬品用途)」2012

    • Inventor(s)
      道上宏之、林圭一郎、松井秀樹、山田浩司、竹居孝二、宮地弘幸、浅井章良
    • Industrial Property Rights Holder
      道上宏之、林圭一郎、松井秀樹、山田浩司、竹居孝二、宮地弘幸、浅井章良
    • Industrial Property Number
      2012-263317
    • Filing Date
      2012-11-30
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 「抗浸潤薬の新規スクリーニング法および抗浸潤剤」2012

    • Inventor(s)
      山田浩司、竹居孝二、道上宏之、松井秀樹、浅井章良
    • Industrial Property Rights Holder
      山田浩司、竹居孝二、道上宏之、松井秀樹、浅井章良
    • Industrial Property Number
      2012-137489
    • Filing Date
      2012-06-19
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 抗浸潤薬の新規スクリーニング法および抗浸潤剤2012

    • Inventor(s)
      山田浩司、竹居孝二、道上宏之、松井秀樹、浅井章良
    • Industrial Property Rights Holder
      山田浩司、竹居孝二、道上宏之、松井秀樹、浅井章良
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2012-137489
    • Filing Date
      2012-06-19
    • Related Report
      2012 Annual Research Report
  • [Patent(Industrial Property Rights)] フルボキサミンを配合することを特徴とする分子標的型抗癌剤(特に抗脳腫瘍治療薬剤:第二医薬品用途)2012

    • Inventor(s)
      道上宏之ら7人
    • Industrial Property Rights Holder
      道上宏之ら7人
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2012-263317
    • Filing Date
      2012-11-30
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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