Study of Catalytic Mechanism of Hydrogen Activation by Hydrogenase using Neutron Crystal Structure Analysis
Project/Area Number |
22657031
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Single-year Grants |
Research Field |
Structural biochemistry
|
Research Institution | University of Hyogo |
Principal Investigator |
HIGUCHI Yoshiki 兵庫県立大学, 大学院・生命理学研究科, 教授 (90183574)
|
Project Period (FY) |
2010 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥3,420,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,600,000 (Direct Cost: ¥1,600,000)
|
Keywords | タンパク質 / 中性子結晶解析 / 水素代謝 / 反応機構 / ヒドロゲナーゼ / Ni酵素 / プロトン経路 |
Research Abstract |
The perfectly deuterated[NiFe] hydrogenase was tried to prepare by inoculating the sulfate-reducing bacterium in the deuterated media in 2010.However, it was not successful to obtain enough amount of the protein sample for crystallization trial. In 2011-2012, the ordinary(not deuterated) hydrogenase was tried to crystallize using deuterated precipitant in deuterated buffer solution. We have succeeded in preparation of a single crystal with a volume of 4 mm^3.This crystal gave a neutron diffraction pattern at 5.0 Aresolution at 100 K using the neutron beam at Japan Atomic Energy Research Institute. In addition, some parameters for diffraction experiment for large single crystals of[NiFe] hydrogenase at cryo-temperature have been found.
|
Report
(3 results)
Research Products
(94 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Cytochrome c polymerization by successive domain swapping at the C-terminal helix2010
Author(s)
S. Hirota, Y. Hattori, S. Nagao, M. Taketa, H. Komori, H. Kamikubo, Z. Wang, I. Takahashi, S. Negi, Y. Sugiura, M. Kataoka, Y. Higuchi
-
Journal Title
Pro. Natl. Acad. Sci
Volume: 107(29)
Issue: 29
Pages: 12854-12859
DOI
NAID
Related Report
Peer Reviewed
-
[Journal Article] Crystal Structures of Ethanolamine Ammonia-lyase complexed with Coenzyme B_12 Analogs and Substrates2010
Author(s)
N. Shibata, H. Tamagaki, N. Hieda, K. Akita, H. Komori, Y. Shomura, S. Terawaki, K. Mori, N. Yasuoka, Y. Higuchi, and T. Toraya
-
Journal Title
J. Biol. Chem
Volume: 285 (34)
Issue: 34
Pages: 26484-26493
DOI
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] X-ray Crystallographic Analysis of the 6-aminohexanoate Cyclic Dimer Hydrolase: Catalytic Mechanism and Evolution of an Enzyme Responsible for Nylon-6 Byproduct Degradation2010
Author(s)
K. Yasuhira, N. Shibata, G. Mongami, Y. Uedo, Y. Atsumi, Y. Kawashima, A. Hibino, Y. Tanaka, Y-H. Lee, D. Kato, M. Takeo, Y. Higuchi, and S. Negoro
-
Journal Title
J. Biol. Chem
Volume: 285
Issue: 2
Pages: 1239-1248
DOI
Related Report
Peer Reviewed
-
-
-
[Journal Article] Expression, Crystallization and Preliminary X-ray Crystallographic Study of Ethanolamine Ammonia-lyase from Escherichia coli2010
Author(s)
N. Shibata, H. Tamagaki, S. Ohtsuki, N. Hieda, K. Akita, H. Komori, Y. Shomura, S. Terawaki, T. Toraya, N. Yasuoka and Y. Higuchi
-
Journal Title
Acta Crystallogr.
Volume: F66
Issue: 6
Pages: 709-711
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Book] 構造生物学2010
Author(s)
樋口芳樹, 中川敦史
Total Pages
253
Publisher
共立出版
Related Report
-