Analysis of collective cell migration by using zebrafish in vivo imaging

• Project/Area Number: 22657048
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Chiba University (2012-2013); Nagoya University (2010-2011)
• Principal Investigator: ITOH MOTOYUKI 千葉大学, 薬学研究科(研究院), 教授 (20377906)
• Co-Investigator(Renkei-kenkyūsha): KAWAKAMI Koichi 国立遺伝学研究所, 教授 (70195048)
• Project Period (FY): 2010-04-01 – 2013-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,460,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥660,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥600,000 (Direct Cost: ¥600,000)
• Keywords: 細胞骨格・運動 / アンチセンス / 細胞・組織 / ゼブラフィッシュ / 細胞挙動 / Notch

Research Abstract

Collective cell migration plays a role in morphogenesis during development and cancer cell metastasis. However molecular mechanisms underlying its regulation remain uncertain. We used zebrafish lateral line system as a model for collective cell migration and established transgenic lines that express imaging reporters for actin and microtubule dynamics. Furthermore, BNA-modified antisense oligonucleotides was evaluated for their efficacy in gene knockdown in zebrafish. We show a potential application for BNA antisense oligos in the downregulation of specific gene expression in zebrafish.

Research Products

• [Journal Article] Target Gene Knockdown by 2', 4'-BNA/LNA Antisense Oligonucleotides in Zebrafish (2014)
  • Author(s): Itoh M, Nakaura M, Imanishi T, Obika S
  • Journal Title: Nucleic Acid Ther Volume: 24(3) Pages: 186-191
  • Peer Reviewed
• [Journal Article] Target Gene Knockdown by 2',4'-BNA/LNA Antisense Oligonucleotides in Zebrafish (2014)
  • Author(s): Itoh M, Nakaura M, Imanishi T, Obika S
  • Journal Title: Nucleic Acid Ther Volume: -
  • Peer Reviewed
• [Presentation] Notch signaling regulates neuronal versus sensory epithelial fate choice in the zebrafish lateral line system (2011)
  • Author(s): Takamasa Mizoguchi, Satoshi Togawa, Koichi Kawakami and Motoyuki Itoh
  • Organizer: 第17回小型魚類研究会
  • Place of Presentation: 東レ研修センター 三島
  • Year and Date: 2011-09-08
• [Presentation] Notch signaling regulates neuronal versus sensory epithelial fate choice in the zebrafish lateral line system (2011)
  • Author(s): Takamasa Mizoguchi, Satoshi Togawa, Koichi Kawakami, Motoyuki Itoh
  • Organizer: 第17回小型魚類研究会
  • Place of Presentation: 東レ研修センター三島
  • Year and Date: 2011-09-08
• [Presentation] Neuronal versus sensory epithelial fate choice is controlled by Notch signaling in the zebrafish lateral line system (2011)
  • Author(s): Takamasa Mizoguchi, Satoshi Togawa and Motoyuki Itoh
  • Organizer: 日本発生生物学会 第44回大会
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2011-05-19
• [Presentation] Neuronal versus sensory epithelial fate choice is controlled by Notch signaling in the zebrafish lateral line system (2011)
  • Author(s): Takamasa Mizoguchi, Satoshi Togawa, Motoyuki Itoh
  • Organizer: 日本発生生物学会第44回大会
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2011-05-19
• [Presentation] Mib is required for cell migration in zebrafish posterior lateral line (2010)
  • Author(s): 溝口貴正, 伊藤 素行
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸 ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Mib is required for cell migration in zebrafish posterior lateralline (2010)
  • Author(s): 溝口貴正、伊藤素行
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Mib regulates migration of the posterior lateral line primordium in zebrafish (2010)
  • Author(s): 溝口貴正, 伊藤 素行
  • Organizer: 第16回小型魚類研究会
  • Place of Presentation: 埼玉プラザイースト
• [Presentation] Mib regulates migration of the posterior lateral line primordium inzebrafish. (2010)
  • Author(s): 溝口貴正、伊藤素行
  • Organizer: 第16回小型魚類研究会
  • Place of Presentation: 埼玉プラザイースト
• [Remarks] ホームページ
  • URL: http://www.p.chiba-u.ac.jp/lab/seika/index.html
• [Remarks]
  • URL: http://www.bio.nagoya-u.ac.jp/index2.html
• [Remarks]
  • URL: http://www.bio.nagoya-u.ac.jp/index2.html