Analysis of collective cell migration by using zebrafish in vivo imaging
Project/Area Number |
22657048
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Single-year Grants |
Research Field |
Cell biology
|
Research Institution | Chiba University (2012-2013) Nagoya University (2010-2011) |
Principal Investigator |
ITOH MOTOYUKI 千葉大学, 薬学研究科(研究院), 教授 (20377906)
|
Co-Investigator(Renkei-kenkyūsha) |
KAWAKAMI Koichi 国立遺伝学研究所, 教授 (70195048)
|
Project Period (FY) |
2010-04-01 – 2013-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥3,460,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥660,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥600,000 (Direct Cost: ¥600,000)
|
Keywords | 細胞骨格・運動 / アンチセンス / 細胞・組織 / ゼブラフィッシュ / 細胞挙動 / Notch |
Research Abstract |
Collective cell migration plays a role in morphogenesis during development and cancer cell metastasis. However molecular mechanisms underlying its regulation remain uncertain. We used zebrafish lateral line system as a model for collective cell migration and established transgenic lines that express imaging reporters for actin and microtubule dynamics. Furthermore, BNA-modified antisense oligonucleotides was evaluated for their efficacy in gene knockdown in zebrafish. We show a potential application for BNA antisense oligos in the downregulation of specific gene expression in zebrafish.
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Report
(4 results)
Research Products
(13 results)