Analysis of neuroprotective actions using progranulin-deficient neurons
| Project/Area Number |
22658090
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NISHIHARA Masugi 東京大学, 大学院・農学生命科学研究科, 教授 (90145673)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,420,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | 脳・神経 / プログラニュリン / 神経細胞 / 神経前駆細胞 / 神経変性 / 細胞死 / 興奮性アミノ酸 |
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| Research Abstract |
We have found that neurons deficient of progranulin(PGRN), a multifunctional growth factor, are vulnerable to excitotoxicity of glutamate. In the present study, we established the assay system for analyzing neuroprotective actions using cultured neuronal cells derived from PGRN-deficient mice as well as human SH-SY5Y cells whose PGRN was reduced by siRNA. By means of these assay systems, we found that PGRN exerted neuroprotective actions on both cells, but the actions differed between neural progenitor cells and mature neurons, suggesting that PGRN plays a role in protecting neurons from excitotoxicity regardless of species, though its actions may vary depending on the stages of differentiation of neuronal cells.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Accelerated lipofuscinosis and ubiquitination in granulin knockout mice suggests a role for progranulin in successful aging2010
Author(s)
Ahmed Z, Sheng H, Xu YF, Lin WL, Innes A, Yu X, Hou H, Chiba S, Yamanouchi K, Petrucelli L, Nishihara M, Hutton ML, McGowan E, Dickson D, Lewis J
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Journal Title
Am J Pathol
Volume: 177
Issue: 1
Pages: 311-324
DOI
Related Report
Peer Reviewed
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[Presentation] Progranulin reduction affects tau phosphorylation in P301L tau transgenic mice, Society for Neuroscience2011
Author(s)
Hosokawa M, Arai T, Nonaka T, Yamashita M, Suzukake M, Matsuwaki T, Nishihara M, Hasegawa M, Akiyama H
Organizer
41th annual meeting
Place of Presentation
Washington DC, USA
Year and Date
2011-11-14
Related Report
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