Development of transporter-control technology for fermentatio
| Project/Area Number |
22658108
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tohoku University |
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Principal Investigator |
ABE Keietsu 東北大学, 大学院・農学研究科, 教授 (50312624)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,460,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | 代謝工学 / 輸送体 / 物質生産 / 生体膜 |
|---|
| Research Abstract |
Tetragenococcus halophilus AspT catalyzes the electrogenic exchange of L-asparate1-with L-alanine0. We show that the binding sites of L-aspartate and L-alanine are independently present in AspT by means of the kinetic analyses and mutant analyses. Single-cysteine variants of transmemberane 3 of AspT revealed that TM3 was the traslocation pathway for L-aspartate and that some mutants lost L-aspatate transport activities but still catalyzed L-alanine and L-serine transport.
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Report
(3 results)
Research Products
(22 results)
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[Journal Article] Identification of succinate exporter in Corynebacterium glutamicum and its physiological roles under anaerobic conditions2011
Author(s)
Fukui K, C. Koseki, Y. Yamamoto, J. Nakamura, A. Sasahara, R. Yuji, K. Hashiguchi, Y. Usuda, K. Matsui, H. Kojima, and K. Abe
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Journal Title
J. Biotech
Volume: 154
Pages: 25-34
Related Report
Peer Reviewed
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[Journal Article] Identification of succinate exporter in Corynebacterium glutamicum and its physiological roles under anaerobic conditions2011
Author(s)
Fukui K, C.Koseki, Y Yamamoto, J Nakamura, A Sasahara, R.Yuji, K.Hashiguchi, Y.Usuda, K.Matsui, H Kojima, K.Abe
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Journal Title
J.Bioteehnol.
Volume: In press(未定)
Related Report
Peer Reviewed
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