Development of transporter-control technology for fermentatio

• Project/Area Number: 22658108
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: Tohoku University
• Principal Investigator: ABE Keietsu 東北大学, 大学院・農学研究科, 教授 (50312624)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,460,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: 代謝工学 / 輸送体 / 物質生産 / 生体膜

Research Abstract

Tetragenococcus halophilus AspT catalyzes the electrogenic exchange of L-asparate1-with L-alanine0. We show that the binding sites of L-aspartate and L-alanine are independently present in AspT by means of the kinetic analyses and mutant analyses. Single-cysteine variants of transmemberane 3 of AspT revealed that TM3 was the traslocation pathway for L-aspartate and that some mutants lost L-aspatate transport activities but still catalyzed L-alanine and L-serine transport.

Research Products

• [Journal Article] 産業微生物由来のアスパラギン酸:アラニン交換輸送体(AAEx)ファミリーに属する膜輸送体の機能と産業応用 (2012)
  • Author(s): 福井啓太, 七谷圭, 阿部敬悦
  • Journal Title: 化学と生物 Volume: 52 Pages: 111-118
  • NAID: 10030819023
• [Journal Article] Substrate specificity of the aspartate : alanine antiporter (AspT)of Tetragenococcus halophilus in reconstituted liposomes (2011)
  • Author(s): Sasahara A., K. Nanatani, M. Enomoto, S. Kuwahara, and K. Abe
  • Journal Title: J. Biol. Chem Volume: 286 Pages: 29044-29052
  • Peer Reviewed
• [Journal Article] Identification of succinate exporter in Corynebacterium glutamicum and its physiological roles under anaerobic conditions (2011)
  • Author(s): Fukui K, C. Koseki, Y. Yamamoto, J. Nakamura, A. Sasahara, R. Yuji, K. Hashiguchi, Y. Usuda, K. Matsui, H. Kojima, and K. Abe
  • Journal Title: J. Biotech Volume: 154 Pages: 25-34
  • Peer Reviewed
• [Journal Article] Substrate specificity of the aspartate : alanine antiporter (AspT) of Tetragenococcus halophilus in reconstituted liposomes (2011)
  • Author(s): Sasahara A., K.Nanatani, M.Enomoto, S.Kuwahara, K.Abe
  • Journal Title: J.Biol.Chem. Volume: 286 Pages: 29044-29052
  • Peer Reviewed
• [Journal Article] 醤油乳酸菌におけるアスパラギン酸脱炭酸とエネルギー生成乳酸菌 (2011)
  • Author(s): 七谷圭, 阿部敬悦
  • Journal Title: 日本醸造協会誌 Volume: 106 Pages: 190-201
• [Journal Article] Identification of succinate exporter in Corynebacterium glutamicum and its physiological roles under anaerobic conditions (2011)
  • Author(s): Fukui K, C.Koseki, Y Yamamoto, J Nakamura, A Sasahara, R.Yuji, K.Hashiguchi, Y.Usuda, K.Matsui, H Kojima, K.Abe
  • Journal Title: J.Bioteehnol. Volume: In press(未定)
  • Peer Reviewed
• [Presentation] 醤油乳酸菌Tetragenococcus halophilusのaspartate : alanine交換輸送体AspTのTM3変異体を用いた基質認識機構の解析 (2012)
  • Author(s): 木村拓哉、笹原綾子、七谷圭、阿部敬悦
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都女子大学(京都)
• [Presentation] Structure, function and application of Aspartate : Alanine Exchanger (AAEx) family transporters from industrial microorganisms (2012)
  • Author(s): 七谷圭、福井啓太、阿部敬悦
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都女子大学(京都)
• [Presentation] Energy generation coupled with decarboxylation reactions in lactic acid bacteria-Aspartate : Alanine Antiporter AspT (2011)
  • Author(s): Keietsu Abe, Kei Nanatani
  • Organizer: IUMS 2011
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2011-09-10
• [Presentation] Energy generation coupled with decarboxylation reactions in lactic acid bacteria (2011)
  • Author(s): Keietsu Abe and Kei Nanatani
  • Organizer: IUMS2011
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2011-09-09
• [Presentation] Energy generation coupled with decarboxylation reactions in lactic acid bacteria (2011)
  • Author(s): Keietsu Abe, Kei Nanatani
  • Organizer: IUMS 2011
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2011-09-09
• [Presentation] 乳酸菌Tetragenococcus halophilusのaspartate : alanine 交換輸送体AspTの第3膜貫通領域変異体を用いた基質認識機構の解析 (2011)
  • Author(s): 木村拓哉、笹原綾子、七谷圭、阿部敬悦
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都)
• [Presentation] 細菌由来アスパラギン酸:アラニン交換輸送体AspTのproteoliposomeを用いたKinetics解析 (2011)
  • Author(s): 阿部敬悦
  • Organizer: トランスポーター研究会
  • Place of Presentation: 東北大学(仙台)
• [Presentation] 醤油乳酸菌Tetragenococcus halophilusのaspartate : alanine交換輸送体AspTのTM3における基質認識メカニズムの解析 (2011)
  • Author(s): 木村拓哉、笹原綾子、七谷圭、阿部敬悦
  • Organizer: トランスポーター研究会
  • Place of Presentation: 東北大学(仙台)
• [Presentation] 乳酸菌Tetragenococcus halophilusのaspartate : alanine交換輸送体AspTの第3膜貫通領域変異体を用いた基質認識機構の解析 (2011)
  • Author(s): 木村拓哉、笹原綾子、七谷圭、阿部敬悦
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都)
• [Presentation] 醤油乳酸菌Tetragenococcus hlophilus由来のAspartate : Alanine交換輸送体、AspT の基質特異性解 (2010)
  • Author(s): 笹原綾子、木村拓哉、七谷圭、阿部敬悦
  • Organizer: トランスポーター研究会2010
  • Place of Presentation: 東京医科歯科大学, 東京
• [Book] Energy generation coupled with decarboxylation reactions in lactic acid bacteria. Lactic Acid Bacteria and Bifidobacteria : Current Progress in Advanced Research (2011)
  • Author(s): Nanatani K., and Abe K.
  • Publisher: Caister Academic Press
• [Book] Lactic Acid Bacteria and Bifidobacteria : Current Progress in Advanced Research Chapter 4 pp.67-87, "Energy generation coupled with decarboxylation reactions in lactic acid bacteria."(Yokota A.and Sonomoto K.edts.) (2011)
  • Author(s): Nanatani K., Abe K.
  • Publisher: Caister Academic Press
• [Book] その他のエネルギー獲得系-有機酸・アミノ酸の脱炭酸, 「乳酸菌とビフィズス菌のサイエンス」 (2010)
  • Author(s): 七谷圭, 阿部敬悦
  • Publisher: 京都大学学術出版会
• [Book] 糖の輸送系とカタボライト制御, 「乳酸菌とビフィズス菌のサイエンス」 (2010)
  • Author(s): 阿部敬悦
  • Publisher: 京都大学学術出版会
• [Book] 糖の輸送系とカタボライト制御(「乳酸菌とビフィズス菌のサイエンス」) (2010)
  • Author(s): 阿部敬悦
  • Publisher: 京都大学学術出版会
• [Book] その他のエネルギー獲得系一有機酸・アミノ酸の脱炭酸,「乳酸菌とビフィズス菌のサイエンス」 (2010)
  • Author(s): 七谷圭、阿部敬悦
  • Publisher: 京都大学学術出版会