| Project/Area Number |
22659040
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Kenichi 京都府立医科大学, 医学研究科, 准教授 (40315932)
TSUJIMURA Atsushi 京都府立医科大学, 医学研究科, 助教 (50236890)
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| Co-Investigator(Renkei-kenkyūsha) |
KAKU Natsuko 堀場製作所,大阪大学, 免疫フロンティアセンター, 元研究員,元特任助教
MORISAKI Shinsuke 京都府立医科大学, 医学研究科, 助教 (20627294)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,090,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | 細胞機能形態学 / ホルモン分子 / 核受容体 / 膜受容体 / 臨界期 / 行動内分泌 / 非標識的可視化 / ラマン散乱光 / スペクトル / リガンド / ステロイドホルモン / 受容体 / 結合様式 / シグナル検出 |
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| Research Abstract |
We tried to investigate the interaction of steroid hormones and their receptor by Raman spectrometer analysis and challenged the possibility to visualize the discrimination of axon and myelin sheath of the sciatic nerve by this method. We got specific spectrum of estradiol and testosterone, but dilution protocol to apply for the in vitro study remains to be settled. Appropriate condition by Raman analysis to distinguish the axon and myelin was identified.
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