Steroid hormones and their receptor interaction by Raman spectrometer analysis

• Project/Area Number: 22659040
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: KAWATA Mitsuhiro 京都府立医科大学, 医学研究科, 教授 (60112512)
• Co-Investigator(Kenkyū-buntansha): MATSUDA Kenichi 京都府立医科大学, 医学研究科, 准教授 (40315932) ; TSUJIMURA Atsushi 京都府立医科大学, 医学研究科, 助教 (50236890)
• Co-Investigator(Renkei-kenkyūsha): KAKU Natsuko 堀場製作所,大阪大学, 免疫フロンティアセンター, 元研究員,元特任助教 ; MORISAKI Shinsuke 京都府立医科大学, 医学研究科, 助教 (20627294)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,090,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: 細胞機能形態学 / ホルモン分子 / 核受容体 / 膜受容体 / 臨界期 / 行動内分泌 / 非標識的可視化 / ラマン散乱光 / スペクトル / リガンド / ステロイドホルモン / 受容体 / 結合様式 / シグナル検出

Research Abstract

We tried to investigate the interaction of steroid hormones and their receptor by Raman spectrometer analysis and challenged the possibility to visualize the discrimination of axon and myelin sheath of the sciatic nerve by this method. We got specific spectrum of estradiol and testosterone, but dilution protocol to apply for the in vitro study remains to be settled. Appropriate condition by Raman analysis to distinguish the axon and myelin was identified.

Research Products

• [Journal Article] Immunohistochemical analysis of the effects of estrogen on intraarticular neurogenic inflammation in a rat anterior cruciate ligament transection model of osteoarthritis (2012)
  • Author(s): Yoshida A, Morihara T, Matsuda K I, Sakamoto H, Arai Y, Kida Y, Kawata M, and Kubo T
  • Journal Title: Connect Tissue Res Volume: 53 Issue: 3 Pages: 197-206
  • DOI: 10.3109/03008207.2011.628059
  • Peer Reviewed
• [Journal Article] Positive effect on bone fusion by the combination of platelet-rich plasma and a gelatin beta-tricalcium phosphate sponge : a study using a posterolateral fusion model of lumbar vertebrae in rats (2012)
  • Author(s): Okamoto S I, Ikeda T, Sawamura K, Nagae M, Hase H, Mikami Y, Tabata Y, Matsuda K I, Kawata M, and Kubo T
  • Journal Title: Tissue Eng Part A Volume: 18 Issue: 1-2 Pages: 157-66
  • DOI: 10.1089/ten.tea.2011.0283
  • Peer Reviewed
• [Journal Article] Rapid signaling of steroid hormones in the vertebrate nervous system (2012)
  • Author(s): Sakamoto H, Takahashi H, Matsuda K I, Nishi M, Takanami K, Ogoshi M, Sakamoto T, Kawata M
  • Journal Title: Frontiers in Bioscience Volume: 17 Issue: 1 Pages: 996-1019
  • DOI: 10.2741/3970
  • Peer Reviewed
• [Journal Article] Histone deacetylation during brain development is essential for permanent masculinization of sexual behavior (2011)
  • Author(s): Matsuda K I, Mori H, Nugent B M, Pfaff D W, McCarthy M M, and Kawata M
  • Journal Title: Endocrinology Volume: 152 Pages: 2760-2767
  • Peer Reviewed
• [Journal Article] Highly visible expression of an oxytocin-monomeric red fluorescent protein 1 fusion gene in the hypothalamus and posterior pituitary of transgenic rats (2011)
  • Author(s): Katoh A, Fujihara H, Ohbuchi T, Onaka T, Hashimoto T, Kawata M, Suzuki H, Ueta Y
  • Journal Title: Endocrinology Volume: 152 Pages: 2768-2774
  • Peer Reviewed
• [Journal Article] Intranuclear dynamics of corticosteroid receptors and effects of proteasomal activity in cultured hippocampal neural cells (2011)
  • Author(s): Nishi M, Noriko H H, and Kawata M
  • Journal Title: Neurosci Lett Volume: 494 Issue: 1 Pages: 65-69
  • DOI: 10.1016/j.neulet.2011.02.058
  • Peer Reviewed
• [Journal Article] In vivo assessment of peripheral nerve regeneration by diffusion tensor imaging (2011)
  • Author(s): Morisaki S, Kawai Y, Umeda M, Nishi M, Oda R, Fujiwara H, Yamada K, Higuchi T, Tanaka C, Kawata M, Kubo T
  • Journal Title: J Magn Reson Imaging Volume: Vol.33 Issue: 3 Pages: 535-542
  • DOI: 10.1002/jmri.22442
  • Peer Reviewed
• [Journal Article] Histone Deacetylation during Brain Development Is Essential for Permanent Masculinization of Sexual Behavior (2011)
  • Author(s): Matsuda K I, Mori H, Nugent B M, Pfaff D W, McCarthy M M, Kawata M
  • Journal Title: Endocrinology Volume: 152 Pages: 2760-2767
  • Peer Reviewed
• [Journal Article] Intrauterine position affects estrogen receptor alpha expression in the ventromedial nucleus of the hypothalamus v ia promoter DNA methylation (2010)
  • Author(s): Mori H, Matsuda K, Tsukahara S, Kawata M
  • Journal Title: Endocrinology Volume: 151 Issue: 12 Pages: 5775-5781
  • DOI: 10.1210/en.2010-0646
  • Peer Reviewed
• [Journal Article] Endogenous glucocorticoids improve myelination via Schwann cells after peripheral nerve injury : An in vivo study using a crush injury model (2010)
  • Author(s): Morisaki S, Nishi M, Fujiwara H, Oda R, Kawata M, and Kubo T
  • Journal Title: Glia Volume: 58 Issue: 8 Pages: 954-963
  • DOI: 10.1002/glia.20977
  • Peer Reviewed
• [Journal Article] Induction of the arginine vasopressin-enhanced green fluorescent protein fusion transgene in the rat locus coeruleus (2010)
  • Author(s): Todoroki M, Ueta Y, Fujihara H, Otsubo H, Shibata M, Hashimoto H, Kobayashi M, Sakamoto H, Kawata M, Dayanithi G, Murphy D, Hiro H, Takahashi K, and Nagata S
  • Journal Title: Stress Volume: 13 Issue: 4 Pages: 281-291
  • DOI: 10.3109/10253890903383406
  • Peer Reviewed
• [Journal Article] Intrauterine position affects estrogen receptor alpha expression in the ventromedial nucleus of the hypothalamus via promoter DNA methylation. (2010)
  • Author(s): Mori H, Matsuda K, Tsukahara S, Kawata M
  • Journal Title: Endocrinology Volume: 151 Pages: 5775-5781
  • Peer Reviewed
• [Journal Article] Endogenous glucocorticoids improve myelination via Schwann cells after peripheral nerve injury : An in vivo study using a crush injury model. (2010)
  • Author(s): Morisaki S, Nishi M, Fujiwara H, Oda R, Kawata M, Kubo T
  • Journal Title: Glia Volume: 58 Pages: 954-963
  • Peer Reviewed
• [Journal Article] High-voltage electron microscopy reveals direct synaptic inputs from a spinal gastrin-releasing peptide system to neurons of the spinal nucleus of bulbocavernosus. (2010)
  • Author(s): Sakamoto H, Arii T, Kawata M
  • Journal Title: Endocrinology Volume: 151 Pages: 417-421
  • Peer Reviewed
• [Journal Article] Expression of G protein-coupled receptor 30 in the spinal somato sensory system. (2010)
  • Author(s): Takanami K, Sakamoto H, Matsuda K, Hosokawa K, Nishi M, Prossnitz E R, Kawata M
  • Journal Title: Brain Res Volume: 1310 Pages: 17-28
  • Peer Reviewed
• [Presentation] 転写共役因子SAFB2による、エストロゲン受容体の動態と機能抑制 (2011)
  • Author(s): 橋本隆, 松田賢一, 河田光博
  • Organizer: 第1回4大学連携研究フォーラム
  • Place of Presentation: 京都
  • Year and Date: 2011-12-09
• [Presentation] 海馬CA1の錐体細胞に対するテストステロンの作用 (2011)
  • Author(s): 李美花, 馬杉(時田)美和子, 河田光博
  • Organizer: 第1回4大学連携研究フォーラム
  • Place of Presentation: 京都
  • Year and Date: 2011-12-09
• [Presentation] アンドロゲン受容体の細胞内動態と性機能への関与 (2011)
  • Author(s): 河田光博
  • Organizer: 第11回日本抗加齢医学会総会
  • Place of Presentation: 京都
• [Presentation] 性ホルモン作用と受容体、行動制御の分子メカニズム (2011)
  • Author(s): 河田光博
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] 神経系と内分泌系の接点から生体システムを考える:分子イメージング、形態、機能、そして行動 (2011)
  • Author(s): 河田光博
  • Organizer: 日本下垂体研究会第26回学術集会
  • Place of Presentation: 岡山
• [Presentation] 基礎医学からみた神経内分泌学の将来 (2011)
  • Author(s): 河田光博
  • Organizer: 第38回日本神経内分泌学会学術集会
  • Place of Presentation: 東京
• [Presentation] 子宮内環境がラット視床下部エストロゲン受容体の分布に及ぼす影響について (2011)
  • Author(s): 森浩子, 松田賢一, 塚原伸治, 河田光博
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] 心的外傷後ストレス障害モデル動物では視索上核オキシトシン発現は減少する (2011)
  • Author(s): 吉井崇喜, 松田賢一, 橋本隆, 西村伊三男, 石田拓也, 土田英人, 河田光博, 福居顯二
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] PTSDモデルラットの脳内におけるCRHの発現変化 (2011)
  • Author(s): 橋本隆, 松田賢一, 河田光博
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] オキシトシンニューロンの脊髄gastrin-releasing peptideニューロン系への遠心性投射の解析 (2011)
  • Author(s): 越智拓海, 佐藤慧太, 佐藤佳亮, 西森克彦, 坂本竜哉, 松田賢一, 河田光博, 坂本浩隆
  • Organizer: 第38回日本神経内分泌学会学術集会
  • Place of Presentation: 東京
• [Presentation] ラット泌乳期後半におけるパルス状黄体形成ホルモン分泌と弓状核キスペプチン発現はエストロゲン依存性に抑制される (2011)
  • Author(s): 山田俊児, 岩田衣世, 上野山賀久, 前多敬一郎, 東村博子, 河田光博
  • Organizer: 第38回日本神経内分泌学会学術集会
  • Place of Presentation: 東京
• [Presentation] アンドロゲン受容体の細胞内動態 (2010)
  • Author(s): 河田光博
  • Organizer: 第1回テストステロン研究会(第10回日本Men's Health医学会)
  • Place of Presentation: 東京
  • Year and Date: 2010-11-28
• [Presentation] アンドロゲン受容体の細胞内動態 (2010)
  • Author(s): 河田光博
  • Organizer: 第1回テストステロン研究会(第10回日本Men's Health医学会)
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2010-11-28
• [Presentation] Sexual Neuroendocrinology : Sex Steroid Hormone Receptor Dynamism, Distribution and Functional Significance (2010)
  • Author(s): Kawata M
  • Organizer: IBRO School of Neuroscience
  • Place of Presentation: Selangor Darul Ehsan, Malaysia
• [Presentation] Histone deacetylase activity is involved in the masculinization of the developing rodent brarin. (2010)
  • Author(s): Matsuda K, Mori H, Nugent BM, McCarthy MM, Kawata M
  • Organizer: The 7th International Congress of Neuroendocrinology
  • Place of Presentation: Rouen, France
• [Presentation] A novel hypothalamic nucleus : Sagittalis nucleus exhibits sexual dimorphism and hormonal responsiveness. (2010)
  • Author(s): Mori H, Matsda K, Pfaff DW, Kawata M
  • Organizer: The 7th International Congress of Neuroendocrinology
  • Place of Presentation: Rouen, France
• [Presentation] Sexual Neuroendocrinology : Sex Steroid Hormone Receptor Dynamism, Distribution and Functional Significance. (2010)
  • Author(s): Kawata M
  • Organizer: IBRO School of Neuroscience
  • Place of Presentation: Selangor Darul Ehsan Malaysia(招待講演)
• [Presentation] エストロゲンによる痒覚調節 (2010)
  • Author(s): 高浪景子, 松田賢一, 坂本浩隆, 平原幸恵, 河田光博
  • Organizer: Neuro2010(第33回日本神経科学大会,第53回日本神経化学会大会,第20回日本神経回路学会大会合同大会)
  • Place of Presentation: 兵庫
• [Presentation] mGluRノックアウトマウスにおける社会的行動の解析 (2010)
  • Author(s): 馬杉(時田)美和子, Peter Josef Flor, 河田光博
  • Organizer: Neuro2010(第33回日本神経科学大会、第53回日本神経化学会大会,第20回日本神経回路学会大会合同大会)
  • Place of Presentation: 兵庫
• [Remarks]
  • URL: http://www.f.kpu-m.ac.jp/k/anat1/index.html
• [Remarks]
  • URL: http://www.f.kpu-m.ac.jp/k/anatl/index.html