| Project/Area Number |
22659060
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Kochi University |
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Principal Investigator |
HONKE Koichi 高知大学, 教育研究部・医療学系, 教授 (80190263)
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,190,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | 生体分子医学 / 分子間相互作用 / 生体膜 / 質量分析 / 抗体医薬品 / プロテオーム / 生体分子 |
|---|
| Research Abstract |
In order to improve the recently established method to analyze molecular interactions in the plasma membranes, called EMARS, we developed the second generation labeling reagent and enabled to identify proteins labeled by the EMARS method by using mass spectrometry-based proteomics technology. Furthermore, we found changes in molecular interactions in the plasma membranes elicited by stimulations with extracellular matrix proteins or therapeutic antibodies and elucidated the involvement of novel molecules in the signal pathway leading to the biological effects.
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