Highly sensitive diagnosis for virus based on next generation of hybridoma technology
Project/Area Number |
22659115
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Single-year Grants |
Research Field |
Laboratory medicine
|
Research Institution | Mie University |
Principal Investigator |
TOMITA Masahiro 三重大学, 大学院・工学研究科, 教授 (20183494)
|
Research Collaborator |
AKACHI Shigehiro
|
Project Period (FY) |
2010 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,300,000)
|
Keywords | インフルエンザウイルス / 電気パルス融合 / ハイブリドーマテクノロジー / ビオチン/アビジン架橋 / モノクローナル抗体 / B細胞選択 / ELISA 法 / HI 試験法 / ELlSA法 / HIテスト / ELISA法 |
Research Abstract |
Three different types of influenza virus such as A/California/07/2009(H1N1), A/Brisbane/59/2007(H1N1) and A/Uruguay/716/2007(H3N2) were employed for immunization. As a result, each anti-serum indicated specificity to its own type of influenza virus by hemagglutination inhibition (HI) test. Selective production of monoclonal antibodies was carried out by use of next generation of hybridoma technology and some promising hybridoma cells secreting aimed monoclonal antibodies were obtained.
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Report
(3 results)
Research Products
(71 results)