The Development of a rapid genetic diagnostic system and the application to the definitive diagnosis of influenza subtypes
| Project/Area Number |
22659118
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | 財団法人東京都医学総合研究所 (2011)
Tokyo Metropolitan Organization for Medical Research (2010) |
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Principal Investigator |
NAITO Akihiro 財団法人東京都医学総合研究所, ゲノム医科学研究分野, 研究員 (20332372)
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| Co-Investigator(Kenkyū-buntansha) |
SAKURAI Akira 財団法人東京都医学総合研究所, ゲノム医科学研究分野, 研究員 (40546628)
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| Co-Investigator(Renkei-kenkyūsha) |
NOMURA Namiko 財団法人東京都医学総合研究所, ゲノム医科学研究分野, 研究補助員 (50599694)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,090,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | 臨床検査システム / 遺伝子診断 / 新型インフルエンザ / 臨床診断 / インフルエンザ / PCR / 迅速診断 |
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| Research Abstract |
Usually, it takes longer than two hours for the definitive diagnosis of the subtypes of influenza viruses by conventional real time PCRs. This newly developed system, however, enables to genotype of influenza viruses in clinical samples in less than 30 min. for whole processes starting from RNA extraction. Although the specificity of the system was slightly lower than that of conventional real time PCRs, it is suggested that the new system is able to identify H1N1 or H3N2 and oseltamivir(Tamiflu) susceptible or resistant in the clinical samples.
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Report
(3 results)
Research Products
(2 results)
