| Project/Area Number |
22659124
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Hygiene
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| Research Institution | Kochi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HINO Okio 順天堂大学, 医学部, 教授 (90127910)
KONDO Tadashi 国立がんセンター研究所, プロテオミクスバイオインフォマティクス, プロジェクトリーダー (30284061)
HIROTA Ryoji 高知大学, 教育研究部・医療学系, 助教 (20448385)
EITOKU Masamitsu 高知大学, 教育研究部・医療学系, 助教 (50552733)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | 産業衛生 / プロテオミクス / 中皮腫 / バイオマーカー / プロテオーム / マイクロアレイ |
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| Research Abstract |
Protein array method was applied to develop biomarker measurement system. Manual arrayer was used to spot human plasma sample and HRP labeled anti-mesothelin antibody was applied. The system detects the protein at C terminal, while the available ELISA uses two antibodies for N and C terminals. The results from the two methods did not correlate each other. Further analysis using other clinical data including radiological findings is now underway. Optimization of protein array measurement to reduce processing time is also needed.
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