Identification of antigen of IgA and Membranous Nephropathy by On -site proteomics

• Project/Area Number: 22659165
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Kidney internal medicine
• Research Institution: Niigata University
• Principal Investigator: XU Bo 新潟大学, 医歯学総合研究科, 研究員 (70463982)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Tadashi 新潟大学, 医歯学系, 教授 (30092737) ; ZHANG Ying 新潟大学, 医歯学総合研究科, 研究員 (00529472)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,300,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: IgA腎症 / 膜性腎症 / プロテオミクス / On-siteプロテオミクス

Research Abstract

Formation of immune complexes were recognized as key rule involved with IgA nephropathy and membranous nephropathy deeply, but it is still unclear in many cases of antigen of these diseases. In this study, 10 μm thickness sections of kidney biopsy samples were pretreated, and glomeruli were dissected by using laser micro dissection (LMD). Peptides were extracted from collected glomeruli followed by On-Site proteomics method followed LC-MS/MS analysis. Proteins with significant changesbetween the IgA・membranous nephropathy and normal glomerulus were analyzed by bioinformatics tool and pathway analysis of diseases to be explored their pathogenesis of IgA・membranous nephropathy

Research Products

• [Journal Article] Extracellular heat shock protein A9 is a novel interaction partner of podoplanin in oral squamous cell carcinoma cells (2013)
  • Author(s): Masayuki Tsuneki
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 434 Issue: 1 Pages: 124-130
  • DOI: 10.1016/j.bbrc.2013.03.057
  • Peer Reviewed
• [Journal Article] Profiling of kidney vascular endothelial cell plasma membrane proteins by liquid chromatography-tandem mass spectrometry (2012)
  • Author(s): Liu Z, Xu B, Nameta M, Zhang Y, Magdeldin S, Yoshida Y, Yamamoto K, Fujinaka H, Yaoita E, Tasaki M, Nakagawa Y, Saito K, Takahashi K, Yamamoto T
  • Journal Title: Clin Exp Nephrol Volume: 掲載予定 Issue: 3 Pages: 327-337
  • DOI: 10.1007/s10157-012-0708-1
  • NAID: 10031184238
  • Peer Reviewed
• [Journal Article] Murine colon proteome and characterization of the protein pathways. (2012)
  • Author(s): Magdeldin S
  • Journal Title: BioData Min. Volume: 5 Issue: 1 Pages: 11-11
  • DOI: 10.1186/1756-0381-5-11
  • Peer Reviewed
• [Journal Article] Extensive proteomic profiling of the secretome of European community acquired methicillin resistant Staphylococcus aureus clone (2012)
  • Author(s): Enany S, Yoshida Y, Magdeldin S, Zhang Y, Xu B, Yamamoto T
  • Journal Title: Peptides Volume: 37 Issue: 1 Pages: 128-37
  • DOI: 10.1016/j.peptides.2012.06.011
  • Peer Reviewed
• [Journal Article] Proteomic approach to human kidney glomerulus prepared by laser microdissection from frozen biopsy specimens: exploration of proteome after removal of blood-derived proteins (2012)
  • Author(s): Yoshida Y, Nameta M, Kuwano M, Zhang Y, Bo X, Magdeldin S, Cui Z, Fujinaka H, Yaoita E, Tomonaga T, Yamamoto T
  • Journal Title: Proteomics Clin Appl Volume: 6 Issue: 7-8 Pages: 412-417
  • DOI: 10.1002/prca.201200016
  • Peer Reviewed
• [Journal Article] "All and None" Refining Strategy; Fishing Your Correct Protein from Proteomics Ocean (2011)
  • Author(s): Magdeldin S, Yoshida Y, Zhang Y, Xu B, Yaoita E, Yamamoto T
  • Journal Title: J Proteomics Bioinform Volume: 4 Pages: 123-124
  • Peer Reviewed
• [Journal Article] Comparison of human glomerulus proteomic profiles obtained from low quantities of samples by different mass spectrometry with the comprehe nsive database (2011)
  • Author(s): Ying Zhang
  • Journal Title: Proteome Science Volume: VOL.9 Issue: 1 Pages: 47-57
  • DOI: 10.1186/1477-5956-9-47
  • Peer Reviewed
• [Journal Article] Usage of electrostatic eliminator reduces human keratin contamination significantly in gel-based proteomics analysis (2011)
  • Author(s): 許波
  • Journal Title: Journal of Proteomics Volume: VOL.74 Issue: 7 Pages: 1022-1029
  • DOI: 10.1016/j.jprot.2011.03.001
  • Peer Reviewed
• [Journal Article] Novel expression of claudin-5 in glomerular podocytes (2011)
  • Author(s): Koda R, Zhao L, Yaoita E, Yoshida Y, Tsukita S, Tamura A, Nameta M, Zhang Y, Fujinaka H, Magdeldin S, Xu B, Narita I, Yamamoto T
  • Journal Title: Cell Tissue Res Volume: 343(3) Issue: 3 Pages: 637-48
  • DOI: 10.1007/s00441-010-1117-y
  • Peer Reviewed
• [Journal Article] プロテオミクスの試料調製 (2010)
  • Author(s): 許波
  • Journal Title: 日本腎臓学会誌 Volume: 52 Pages: 495-498
  • NAID: 10026409300
  • Peer Reviewed
• [Journal Article] Comparison of two dimensional electrophoresis mouse colon proteomes before and after knocking out Aquaporin 8 (2010)
  • Author(s): Magdeldin S, Li H, Yoshida Y, Enany S, Zhang YXB, Fujinaka H, Yaoita E, Yamamoto T.
  • Journal Title: Journal of Proteomics Volume: 73 Pages: 2031-2040
  • Peer Reviewed
• [Journal Article] Usage of electrostatic eliminator reduces human keratin contamination significantly in gel-based proteomics analysis.
  • Author(s): Bo Xu, Ying Zhang, Zongjiang Zhao, Yutaka Yoshida, Sameh Magdeldin, Hidehiko Fujinaka, Tamer Ahmed Ismail, Eishin Yaoita, Tadashi Yamamoto.
  • Journal Title: Journal of Proteomics Volume: In Press
  • Peer Reviewed
• [Presentation] Proteomics analysis of laser microdissected FFPE glomerular sections from human IgA nephropathy biopsy specimens (2012)
  • Author(s): Xu B, Shimada S, Zhang Y, Yoshida Y, Liu Z, Magdeldin S, Fujinaka H, Yaoita E, Yamamoto T
  • Organizer: 第11回国際ヒトプロテオーム機構学術集会
  • Place of Presentation: ボス トン(アメリカ合衆国)
• [Presentation] Proteomics analysis of laser microdissected FFPE glomerular sections from human IgA nephropathy biopsy specimens. (2012)
  • Author(s): Xu Bo
  • Organizer: 11th Annual World Congress of Human Proteome Organization (HUPO)
  • Place of Presentation: BOSTON, USA
• [Presentation] Comparison of on-site proteomics study on laser microdissected FFPE and frozen human glomerulus tissues (2011)
  • Author(s): Xu B, Shimada S, Zhang Y, Yamamoto T
  • Organizer: 10th Annual World Congress of Human Proteome Organization (HUPO)
  • Place of Presentation: Room K, Geneva Palexpo, Geneva, Switzerland
  • Year and Date: 2011-09-06
• [Presentation] Proteome research of murine liver tissues of NASH model mouse (2011)
  • Author(s): Xu B, Zhang Y, Azlina E, Yamamoto T., et al
  • Organizer: 9th Japan Human Proteomics Organization (JHUPO) Confernce
  • Place of Presentation: Snow Hall A, Toki Messe, Niigata, Japan
  • Year and Date: 2011-07-29
• [Presentation] Proteomics results of small FFPE rat cortex tissues treated by 3 methods (2011)
  • Author(s): Xu B, Zhang Y, Yaoita E, Yamamot T
  • Organizer: 9th Japan Human Proteomics Organization (JHUPO) Confernce
  • Place of Presentation: Snow Hall A, Toki Messe, Niigata, Japan
  • Year and Date: 2011-07-29
• [Presentation] Preparation of sample for proteomics research (2011)
  • Author(s): Xu B, Yoshida Y, Yamamoto T
  • Organizer: 9th Japan Human Proteomics Organization (JHUPO) Confernce
  • Place of Presentation: Room 201, Toki Messe, Niigata(招待講演)
  • Year and Date: 2011-07-28
• [Presentation] A VBA program for extracting keratin contamination from protecome analysis results (2011)
  • Author(s): Makiguchi T, Xu B, Yoshida Y, Yamamoto T
  • Organizer: 9th Japan Human Proteomics Organization (JHUPO) Confernce
  • Place of Presentation: Snow Hall A, Toki Messe, Niigata, Japan
  • Year and Date: 2011-07-28
• [Presentation] Processing tool works on data of identified proteins exported by ions search engine (2011)
  • Author(s): Ikoma T,Xu B, Yamamoto T
  • Organizer: 9th Japan Human Proteomics Organization (JHUPO) Confernce
  • Place of Presentation: Snow Hall A, Toki Messe, Niigata, Japan
  • Year and Date: 2011-07-28
• [Presentation] Proteomic mapping oT kidney and liver endotheliai cell surface proteins (2011)
  • Author(s): Liu Z, Xu B, Zhang Y, Yoshida Y, Yamamoto T
  • Organizer: 9th Japan Human Proteomics Organization (JHUPO) Confernce
  • Place of Presentation: Snow Hall A, Toki Messe, Niigata, Japan
  • Year and Date: 2011-07-28
• [Presentation] Comparison of on-site proteomics study on laser microdissected FFPE and frozen human glomerulus tissues (2011)
  • Author(s): Xu B, Shimada S, Zhang Y, Magdeldin S, Fujinaka H, Yoshida Y, Yaoita Y, Yamamoto T
  • Organizer: 第10回国際ヒトプロテオーム機構学術集会
  • Place of Presentation: ジュネーブ(スイス連邦)
• [Presentation] Proteomics results of small FFPE rat cortex tissues treated by 3 methods (2011)
  • Author(s): Xu B, Zhang Y, Shimada S, Maddeldin S, Yoshida Y, Yaoita E, Yamamot T
  • Organizer: 日本プロテオーム学会2011年大会(第9回日本ヒトプロテオーム機構)
  • Place of Presentation: 新潟
• [Presentation] Proteome research of murine liver tissues of NASH model mouse (2011)
  • Author(s): Xu B, Shimada S, Takamura M, Zhang Y, Azlina E, Magdeldin S, Yoshida Y, Yaoita E, Yamamoto T
  • Organizer: 日本プロテオーム学会2011年大会(第9回日本ヒトプロテオーム機構)
  • Place of Presentation: 新潟
• [Presentation] Preparation of sample for proteomics research (2011)
  • Author(s): Xu B, Yoshida Y, Yamamoto T
  • Organizer: 日本プロテオーム学会2011年大会(第9回日本ヒトプロテオーム機構)
  • Place of Presentation: 新潟
• [Presentation] Proteomics analysis on formalin-fixedparaffin-embedded human glomeruli using laser microdissection and on-site direct trypsin digestion (2011)
  • Author(s): Xu B, Shimada S, Zhang Y, Yoshida Y, Yaoita E, Morita T , Yamamoto T
  • Organizer: 第54回日本腎臓学会学術総会
  • Place of Presentation: 横浜
• [Presentation] On-site Proteomics Study on Laser Microdissected Rat Renal Cortex and Human Glomerulus Tissues (2010)
  • Author(s): Bo Xu, Shuichiro Shimada, YingZhang, Sameh Magdaldin, Hideo Fujinaka Yutaka Yoshida, Eisin Yaoita, Tadashi Yamamoto.
  • Organizer: 2010 HUPO 9^<th> Annual World Congress
  • Place of Presentation: Exhibition Hall 5,Sydney Convention and Exhibition Centre,NSW,Australia
  • Year and Date: 2010-09-20
• [Presentation] Laser Microdissection法を用いたホルマリン固定標本のon-siteプロテオーム解析 (2010)
  • Author(s): 許波、嶋田修一郎、張螢、五十島美千子、吉田豊、矢尾板永信、森田俊、山本格
  • Organizer: 日本ヒトプロテオーム機構第8回大会
  • Place of Presentation: インペリアホール・アメジスト&エメラルド、東京ベイホテル東急 1F
  • Year and Date: 2010-07-27
• [Presentation] ホルマリン固定標本とLaser Microdissection法を用いたon-siteプロテオーム解析.;第53回日本腎臓学会.03-06-20. (2010)
  • Author(s): 許波、嶋田修一郎、張螢、五十島美千子、吉田豊、矢尾板永信、山本格
  • Organizer: 第53回日本腎臓学会
  • Place of Presentation: 第6会場(神戸国際会議場502会議室)
  • Year and Date: 2010-06-18
• [Presentation] On-site proteomics study on laser microdissected rat renal cortex and human glomerulus tissues (2010)
  • Author(s): Xu B, Shimada S, Zhang Y, Magdeldin S, Fujinaka H, Yoshida Y, Yaoita E, Yamamoto T
  • Organizer: 9th Annual World Congress of Human Proteome Organization (HUPO)
  • Place of Presentation: シドニー(オーストラリア連邦)
• [Presentation] Proteomics analysis on formalin-fixed paraffin-embedded tissue using laser microdissection and on-site direct trypsin digestion (2010)
  • Author(s): Xu B, Shimada S, Zhang Y, Ikashima M, Yoshida Y, Yaoita E, Morita T , Yamamoto T
  • Organizer: 第8回日本ヒトプロテオーム機構
  • Place of Presentation: 東京
• [Presentation] Proteomics study of formalin fixed paraffin embedded rat kidney by laser microdissection (2010)
  • Author(s): Xu B, Shimada S, Zhang Y, Ikashima M, Yoshida Y, Yaoita E, Yamamoto T
  • Organizer: 第53回日本腎臓学会学術総会
  • Place of Presentation: 神戸
• [Remarks]
  • URL: http://hkupp.kir.jp