| Project/Area Number |
22659180
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Kinki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KANAKURA Yuzuru 大阪大学, 大学院・医学系研究科, 教授 (20177489)
MIZUKI Masao 大阪大学, 附属病院, 准教授 (80283761)
ORITANI Kenji 大阪大学, 大学院・医学系研究科, 准教授 (70324762)
SHIBAYAMA Hirohiko 大阪大学, 大学院・医学系研究科, 講師 (60346202)
ASHIDA Takashi 近畿大学, 医学部・附属病院, 准教授 (30211006)
TATSUMI Yoichi 近畿大学, 医学部・附属病院, 准教授 (60236552)
SHIMADA Takahiro 近畿大学, 医学部, 講師 (90319674)
TANAKA Hirokazu 近畿大学, 医学部, 講師 (40360846)
前田 裕弘 近畿大学, 医学部, 教授 (10173717)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,230,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | 白血病 / 4倍体胚補完法 / 白血病幹細胞 / ES細胞 / 分化 / 白血病原因遺伝子 / トランスジェニックマウス / Tet-offシステム |
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| Research Abstract |
In this study, we generated BCR-ABL transgenic mice using the tetraploid embryonic complementation method, in which we can turn on or off the BCR-ABL gene using the Tet-off system. In these mice, CML-like disease was induced about 1 month after the Tet-off, which was disappeared by Tet-on. The change of the methylation profile was observed two months after Tet-off, indicating that BCR-ABL secondary causes the abnormality in the methylation profile.
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