Establishment of human iPS cells originated from retinal tissues

• Project/Area Number: 22659316
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: AZUMA Noriyuki 独立行政法人国立成育医療研究センター, 眼科医長・細胞医療研究室長 (10159395)
• Co-Investigator(Kenkyū-buntansha): UMEZAWA Akihiro 独)国立成育医療研究センター(研究所), 生殖細胞医療研究部, 部長 (70213486) ; SEKO Yuko 独)国立成育医療研究センター(研究所), 生殖細胞医療研究部, 共同研究員 (60301157)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,320,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: 再生医学 / iPS細胞 / 遺伝子・細胞治療 / 網膜 / 遺伝学 / 角膜

Research Abstract

We generated human iPS cells from the retinal tissues obtained during surgery. By methylation analysis, the iPS cells have characteristics of stem cells similar to those of ES cells. Neural retina cells and retinal pigmented epithelia are identified in teratoma formation. Thus the iPS cells are useful to from highly differentiated retinal cells for reproducing medicine.

Research Products

• [Journal Article] Establishment of functioning human corneal endothelial cell line with high growth potential (2012)
  • Author(s): Yokoi T, Seko Y, Yokoi T, Makino H, Hatou S, Yamada M, Kiyono T, Umezawa A, Nishina H, Azuma N.
  • Journal Title: PLos ONE Volume: 7 Pages: 29677-29677
• [Journal Article] Two novel mutations in the EYS gene are possible major causes of autosomal recessive retinitis pigmentosa in the Japanese population (2012)
  • Author(s): Hosono K, Ishigami C, Takahash M, Park DH, Hirami Y, Nakanishi H, Ueno S, Yokoi T, Hikoya A, Fujita T, Zhao Y, Nishina S, Shin JP, Kim IT, Yamamoto S, Azuma N, Terasaki H, Sato M, Kondo M, Minoshima S, Hotta Y.
  • Journal Title: PLoS ONE Volume: 7
• [Journal Article] Identification of factors determining human photoreceptor cell fate (2012)
  • Author(s): Seko Y, Azuma N, Umezawa A.
  • Journal Title: PLoS ONE Volume: (in press)
• [Journal Article] Clinical features of congenital retinal folds (2012)
  • Author(s): Nishina S、Suzuki Y, Yokoi T, Kobayashi Y, Noda E, Azuma N.
  • Journal Title: Am J Ophthalmol Volume: 153 Pages: 81-87
• [Journal Article] Ophthalmic features of CHARGE syndrome with CHD7 mutations (2012)
  • Author(s): Nishina S, Kosaki R, Yagihashi T, Azuma N, Okamoto N, Hatsukawa Y, Kurosawa K, Yamane T, Mizuno S, Tsuzuki K, Kosaki K.
  • Journal Title: Am J Med Genet Part A Volume: 158A Pages: 514-518
• [Journal Article] Survey of microphthalmia in Japan (2012)
  • Author(s): Nishina S, Kurosaka D, Nishida Y, Kondo H, Kobayashi Y, Azuma N.
  • Journal Title: Jpn J Ophthalmol
  • NAID: 10030626740
• [Journal Article] Clinical features of anterior segment dysgenesis associated with congenital corneal opacities (2012)
  • Author(s): Shigeyasu C, Yamada M, Mizuno Y, Yokoi T, Nishina S, Azuma N
  • Journal Title: Cornea Volume: 31 Pages: 293-298
• [Journal Article] Establishment of functioning human corneal endothelial cell line with high growth potential (2012)
  • Author(s): Yokoi T, Seko Y, Yokoi T, Makino H, Hatou S, Yamada M, Kiyono T, Umezawa A, Nishina H, Azuma N
  • Journal Title: PLoS One Volume: 7(1)
  • Peer Reviewed
• [Journal Article] Derivation of Human Differential Photoreceptor-like Cells from the Iris by Defined Combinations of CRX, RX and NEUROD (2012)
  • Author(s): Seko Y, Azuma N, Kaneda M, Nakatani K, Miyagawa Y, Noshiro Y, Kurokawa R, Okano H, Umezawa A
  • Journal Title: PLoS One Volume: 7(4)
  • NAID: 120007137243
  • Peer Reviewed
• [Journal Article] Two Novel Mutations in the EYS Gene Are Possible Major Causes of Autosomal Recessive Retinitis Pigmentosa in the Japanese Population (2012)
  • Author(s): Hosono K, Azuma N, et al
  • Journal Title: PLoS One Volume: 7(2)
  • Peer Reviewed
• [Journal Article] Clinical features of congenital retinal folds (2012)
  • Author(s): Nishina S, Suzuki Y, Yokoi T, Kobayashi Y, Noda E, Azuma N
  • Journal Title: Am J Ophthalmol Volume: 153(1) Pages: 81-87
  • Peer Reviewed
• [Journal Article] Survey of microphthalmia in Japan (2012)
  • Author(s): Nishina S, Kurosaka D, Nishida Y, Kondo H, Kobayashi Y, Azuma N
  • Journal Title: Jpn J Ophthalmol Volume: Feb23(Equb ahead of print)
  • NAID: 10030626740
  • Peer Reviewed
• [Journal Article] Ophthalmic features of CHARGE syndrome with CHD7 mutations (2012)
  • Author(s): Nishina S, Azuma N, et al
  • Journal Title: Am J Med Genet A Volume: 158A(3) Pages: 514-518
  • Peer Reviewed
• [Journal Article] Stress-activated protein kinase MKK7 regulates axon elongation in the developing cerebral cortex (2011)
  • Author(s): Yamasaki T, Kawasaki H, Arakawa S, Shimizu K, Shimizu S, Reiner O, Okano H, Nishina S, Azuma N, Penninger JM, Katada T, Nishina H.
  • Journal Title: J Neurosci Volume: 31(46) Pages: 16872-16883
• [Journal Article] A case of aniridia with uniocular Peters anomaly (2011)
  • Author(s): Sawada M, Sato M, Hikoya A, Wang C-X, Minoshima S, Azuma N, Hotta Y.
  • Journal Title: J AAPOS Volume: 15 Pages: 104-106
• [Journal Article] Stress-activated protein kinase MKK7 regulates axon elongation in the developing cerebral cortex (2011)
  • Author(s): Yamasaki1 T, Kawasaki H, Arakawa S, Shimizu K, Shimizu S, Reiner O, Okano H, Nishina S, Azuma N, Penninger JM, Katada T, Nishina H.
  • Journal Title: Journal of Neuroscience Volume: 31 Pages: 16872-16883
• [Journal Article] Fluorescein staining of the vitreous during vitrectomy for retinopathy of prematurity (2011)
  • Author(s): Kobayashi Y, Yokoi T, Yokoi T, Hiraoka H, Nishina S, Azuma N
  • Journal Title: Retina Volume: 31 Pages: 1717-1719
  • Peer Reviewed
• [Journal Article] Nishida's procedure combined with medial rectus recession for large-angle esotropia in Duane syndrome (2011)
  • Author(s): Tanaka M, Nishina S, Ogonuki S, Akaike S, Azuma N
  • Journal Title: Jpn J Ophthalmol Volume: 55(3) Pages: 264-267
  • NAID: 10029021414
  • Peer Reviewed
• [Journal Article] Genetic screening of Wnt signaling factors in advanced retinopathy of prematurity (2010)
  • Author(s): Hiraoka M, Takahashi H, Orimo H, Hiraoka M, Ogata T, Azuma N.
  • Journal Title: Mol Vis Volume: 16 Pages: 2572-2577
• [Journal Article] Heterozygous OTX2 mutations are associated with variable pituitary phenotype (2010)
  • Author(s): Dateki S, Kosaka K, Hasegawa K, Tanaka H, Azuma N, Yokoya S, Muroya K, Adachi M, Tajima T, Motomura K, Kinoshita E, Moriuchi H, Fukami M, Ogata T.
  • Journal Title: J Clin Endocrinol Metab Volume: 95 Pages: 756-764
• [Journal Article] p38mitogen-activated protein kinase controls a switch between cardiomyocyte and neuronal commitment of murine embryonic stem cells by activating MEF2C-dependent BMP2 transcription (2010)
  • Author(s): Wu J, Kubota J, Hirayama J, Nagai Y, Nishina S, Yokoi T, Asaoka Y, Seo J, Shimizu N, Kajiho H, Watanabe T, Azuma N, Katada T, Nishina H.
  • Journal Title: Stem Cells Dev Volume: 19 Pages: 1723-1734
• [Journal Article] Risk factors for recurrent fibrovascular proliferation in aggressive posterior retinopathy of prematurity after early vitreous surgery. (2010)
  • Author(s): Yokoi T, Yokoi T, Kobayashi Y, Nishina S, Azuma N.
  • Journal Title: Am J.Ophthalmol Volume: 150 Pages: 10-15
  • Peer Reviewed
• [Journal Article] Heterozygous OTX2 mutations are associated with variable pituitary phenotype. (2010)
  • Author(s): Dateki S, Kosaka K, Hasegawa K, Tanaka H, Azuma N, Yokoya S, Muroya K, Adachi M, Tajima T, Motomura K, Kinoshita E, Moriuchi H, Fukami M, Ogata T
  • Journal Title: J Clin Endocrinol Metab Volume: 95 Pages: 756-764
  • Peer Reviewed
• [Journal Article] p38 mitogen-activated protein kinase controls a switch between cardiomyocyte and neuronal commitment of murine embryonic stem cells by activating MEF2C-dependent BMP2 transcription. (2010)
  • Author(s): Wu J, Kubota J, Hirayama J, Nagai Y, Nishina S, Yokoi T, Asaoka Y, Seo J, Shimizu N, Kajiho H, Watanabe T, Azuma N, Katada T, Nishina H.
  • Journal Title: Stem Cells Dev Volume: In press
  • Peer Reviewed
• [Journal Article] Endogenous Candida chorioretinitis in a healthy infant. (2010)
  • Author(s): Ito M, Yokoi T, Sugita S, Shinohara N, Nishina S, Azuma N.
  • Journal Title: Jpn J Ophthalmol Volume: In press
  • NAID: 10027666584
  • Peer Reviewed
• [Journal Article] Neovascularization in child following laser pinter-induced macular injury. (2010)
  • Author(s): Fujinami K, Yokoi T, Hiraoka M, Nishina S, Azuma N.
  • Journal Title: Jpn J Ophthalmol Volume: In press
  • Peer Reviewed
• [Journal Article] Is a 6-week course of ganciclovir therapy effective for chorioretinitis in infants with congenital cytomegalovirus infection? (2010)
  • Author(s): Shoji K, Ito N, Ito Y, Inoue N, Adachi S, Fujimaru T, Nakamura T, Nishina S, Azuma N, Saitoh A.
  • Journal Title: J Pediatr Volume: In press
  • Peer Reviewed
• [Journal Article] Fluorescein staining of the vitreous during vitrectomy for retinopathy of prematurity. (2010)
  • Author(s): Kobayashi Y, Yokoi T, Yokoi T, Hiraoka H, Nishina S, Azuma N.
  • Journal Title: Retina Volume: In press
  • Peer Reviewed
• [Presentation] 小児眼科のトピックス (2011)
  • Author(s): 東範行
  • Organizer: 湘西眼科臨床フォーラム
• [Presentation] 小児眼科のトピックス (2011)
  • Author(s): 東範行
  • Organizer: 練馬区眼科医会講演会
• [Presentation] 眼を作るシステムと視覚の成立 (2011)
  • Author(s): 東範行
  • Organizer: 群馬県眼科集談会
• [Presentation] 小児の眼底疾患の診かた・考えかた (2011)
  • Author(s): 東範行
  • Organizer: 三重県小児眼科フォーラム
• [Presentation] 小児眼底疾患のトピックス (2011)
  • Author(s): 東範行
  • Organizer: 新宿眼科講演会
• [Presentation] 小児の眼底疾患の診かた・考えかた (2011)
  • Author(s): 東範行
  • Organizer: 鳥取県眼科フォーラム
• [Presentation] 小児眼科のトピックス (2011)
  • Author(s): 東範行
  • Organizer: 第5回北陸オフサルミックフォーラム
• [Presentation] 小児の眼底疾患の診かた・考えかた (2011)
  • Author(s): 東範行
  • Organizer: 広島県眼科集談会
• [Presentation] 未熟児網膜症の診断と治療 (2011)
  • Author(s): 東範行
  • Organizer: あべの眼科セミナー
• [Presentation] 小児眼科のトピックス (2011)
  • Author(s): 東範行
  • Organizer: 青森県眼科集談会
• [Presentation] 眼の形態形成遺伝子と再生医療 (2011)
  • Author(s): 東範行
  • Organizer: CIBAビジョンシンポジウム第114回日本眼科学会
• [Presentation] Analysis of retinal structure and function in eyes Analysis of retinal structure and function in eyes with foveal hypoplasia (2011)
  • Author(s): Yokoi T, Ui R, Ito, Nakayama Y, Nishina S, Azuma N.
  • Organizer: Distinguished investigators' forum, Distinguished investigators' forum第50日本網膜硝子体学会
• [Presentation] 小児眼底疾患のトピックス (2011)
  • Author(s): 東範行
  • Organizer: 眼科アップデートセミナー
• [Presentation] 未熟児網膜症の管理 (2011)
  • Author(s): 東範行
  • Organizer: 第65回日本臨床眼科学会
• [Presentation] 小児の眼底疾患のトピックス (2011)
  • Author(s): 東範行
  • Organizer: 東京都眼科医会講習会
• [Presentation] 先天網膜ひだに伴う裂孔原性網膜剥離 (2011)
  • Author(s): 東範行
  • Organizer: 硝子体手術フォーラム
• [Presentation] 乳児にみられたカンジダ眼内炎の1例 (2011)
  • Author(s): 伊藤牧子,小川学,杉田直,横井匡,小林百合,仁科幸子,東範行
  • Organizer: 第36回日本小児眼科学会
• [Presentation] 強膜内陥術の網膜牽引軽減によって視力が向上した網膜ひだの1症例 (2011)
  • Author(s): 小林百合,伊藤牧子,田中三知子,横井匡,仁科幸子,東範行
  • Organizer: 第36回日本小児眼科学会
• [Presentation] 黄斑低形成の構造と機能 (2011)
  • Author(s): 東範行
  • Organizer: Japan Macula Club
• [Presentation] 先天白内障の手術に関する全国調査 (2011)
  • Author(s): 東範行,不二門尚,石橋達朗,佐藤美保,近藤峰生,大鹿哲郎,黒坂大次郎,永本敏之
  • Organizer: 第65回日本臨床眼科学会
• [Presentation] 永本敏之,東範行,不二門尚,石橋達朗,佐藤美保,近藤峰生,大鹿哲郎,黒坂大次郎 (2011)
  • Author(s): 永本敏之,東範行,不二門尚,石橋達朗,佐藤美保,近藤峰生,大鹿哲郎,黒坂大次郎
  • Organizer: 第65回日本臨床眼科学会
• [Presentation] 先天白内障における眼内レンズ二次挿入に関する多施設アンケート調査 (2011)
  • Author(s): 田中三知子,黒坂大次郎,不二門尚,石橋達朗,佐藤美保,近藤峰生,大鹿哲郎,永本敏之,東範行
  • Organizer: 第65回日本臨床眼科学会
• [Presentation] MRIによる小眼球の検討 (2011)
  • Author(s): 仁科幸子,西田保裕,黒坂大次郎,近藤寛之,東範行
  • Organizer: 第65回日本臨床眼科学会
• [Presentation] 家族性滲出性硝子体網膜症に対するバックリング手術 (2011)
  • Author(s): 宇井理人,伊藤牧子,横井匡,小林百合,仁科幸子,東範行
  • Organizer: 第50日本網膜硝子体学会
• [Presentation] Analysis of retinal structure and function in eyes with foveal hypoplasia (2011)
  • Author(s): Yokoi T, Ui R, Ito M, Nakayama Y, Nishina S, Azuma N
  • Organizer: Distinguished investigators' forum第50日本網膜硝子体学会
  • Place of Presentation: 東京
• [Presentation] Early Vitreous surgery for aggressive posterior retinopathy of prematurity (2010)
  • Author(s): Azuma N.
  • Organizer: Vail Vitreoretinal Meeting
• [Presentation] Ocular manifestations and prognosis of shaken baby syndrome in two Japanese children's hospitals (2010)
  • Author(s): Kobayashi Y, Yamada K, Ohba S, Nishina S, Okuyama M, Azuma N.
  • Organizer: XXXIIth International Congress of Ophthalmology
• [Presentation] Endogenous Candida chorioretinitis in a healthy infant (2010)
  • Author(s): Ito M, Yokoi T, Sugita S, Shinohara N, Nishina S, Azuma N.
  • Organizer: XXXIIth International Congress of Ophthalmology
• [Presentation] Risk factors for recurrent fibrovascular proliferation in aggressive posterior retinopathy of prematurity after early vitreous surgery (2010)
  • Author(s): Yokoi T, Yokoi T, Kobayashi Y, Nishina S, Azuma N.
  • Organizer: XXXIIth International Congress of Ophthalmology
• [Presentation] 未熟児網膜症の診断と治療. (2010)
  • Author(s): 東範行
  • Organizer: 大阪眼科集談会
• [Presentation] 未熟児網膜症の診断と治療 (2010)
  • Author(s): 東範行
  • Organizer: 日本医科大学眼科研究会
• [Presentation] 眼を作るシステムと視覚の成立 (2010)
  • Author(s): 東範行
  • Organizer: 第66回日本弱視斜視学会
• [Presentation] 未熟児網膜症の網膜剥離に対する治療 (2010)
  • Author(s): 東範行
  • Organizer: 第13回静岡網膜硝子体研究会
• [Presentation] 小児の病理眼の発生と形成異常 (2010)
  • Author(s): 東範行
  • Organizer: 第114回日本眼科学会
• [Presentation] 網膜芽細胞腫の化学療法と放射線外照射による治療 (2010)
  • Author(s): 東範行
  • Organizer: 第29回眼腫瘍学会
• [Presentation] 小児眼底の広範な構造と機能の検査 (2010)
  • Author(s): 東範行
  • Organizer: 第64回日本臨床眼科学会
• [Presentation] 未熟児網膜症の管理 (2010)
  • Author(s): 東範行
  • Organizer: 第64回日本臨床眼科学会
• [Presentation] 小児の眼底疾患の診かた・考えかた (2010)
  • Author(s): 東範行
  • Organizer: 東京都眼科医会講習会
• [Presentation] 小児眼底の広範な構造と機能の検査 (2010)
  • Author(s): 伊藤牧子,田中三千子,横井匡,小林百合,野田英一郎,仁科幸子,東範行
  • Organizer: 第35回日本小児眼科学会
• [Presentation] 検眼鏡的に異常がない視力不良例に対する網膜電図検査 (2010)
  • Author(s): 田中三千子,伊藤牧子,横井匡,野田英一郎,小林百合,仁科幸子,東範行
  • Organizer: 第35回日本小児眼科学会
• [Presentation] 小児眼底の広範な構造と機能の検査 (2010)
  • Author(s): 東範行
  • Organizer: Japan Macula Club
• [Presentation] 小眼球の全国統計 (2010)
  • Author(s): 仁科幸子,西田保裕,黒坂大次郎,近藤寛之,東範行
  • Organizer: 第64回日本臨床眼科学会
• [Presentation] 小児の網膜疾患に対する全身麻酔下で局所と全視野の網膜電図所見 (2010)
  • Author(s): 田中三知子,伊藤牧子,横井匡,小林百合,仁科幸子,東範行
  • Organizer: 第58回日本臨床視覚電気生理学会
• [Presentation] 小児における全身麻酔下での局所網膜電図検査 (2010)
  • Author(s): 伊藤牧子,田中三知子,横井匡,小林百合,仁科幸子,東範行
  • Organizer: 第58回日本臨床視覚電気生理学会
• [Presentation] 新型インフルエンザによると思われる滲出性網膜剥離の1例.乳幼児被虐待症候群の眼底所見とその予後 (2010)
  • Author(s): 横井匡,伊藤牧子,小林百合,田中三知子,仁科幸子,杉田直,望月學,東範行
  • Organizer: 第49日本網膜硝子体学会
• [Presentation] 乳頭周囲ぶどう腫、朝顔症候群の黄斑の構造と機能 (2010)
  • Author(s): 田中三知子,伊藤牧子,横井匡,小林百合,仁科幸子,東範行
  • Organizer: 第49日本網膜硝子体学会
• [Presentation] Ocular manifestations and prognosis of shaken baby syndrome in two Japanese children's hospitals. (2010)
  • Author(s): Kobayashi Y, Yamada K, Ohba S, Nishina S, Okuyama M, Azuma N.
  • Organizer: XXXIIth International Congress of Ophthalmology
  • Place of Presentation: ベルリン
• [Presentation] Endogenous Candida chorioretinitis in a healthy infant. (2010)
  • Author(s): Ito M, Yokoi T, Sugita S, Shinohara N, Nishina S, Azuma N.
  • Organizer: XXXIIth International Congress of Ophthalmology
  • Place of Presentation: ベルリン
• [Presentation] Risk factors for recurrent fibrovascular proliferation in aggressive posterior retinopathy of prematurity after early vitreous surgery. (2010)
  • Author(s): Yokoi T, Yokoi T, Kobayashi Y, Nishina S, Azuma N.
  • Organizer: XXXIIth International Congress of Ophthalmology
  • Place of Presentation: ベルリン
• [Presentation] 児の病理 眼の発生と形成異常 (2010)
  • Author(s): 東範行
  • Organizer: 第114回日本眼科学会
  • Place of Presentation: 名古屋
• [Presentation] 小児眼底の広範な構造と機能の検査 (2010)
  • Author(s): 伊藤牧子, 田中三千子, 横井匡, 小林百合, 野田英一郎, 仁科幸子, 東範行
  • Organizer: 第35回日本小児眼科学会
  • Place of Presentation: 東京
• [Presentation] 検眼鏡的に異常がない視力不良例に対する網膜電図検査 (2010)
  • Author(s): 田中三千子, 伊藤牧子, 横井匡, 野田英一郎, 小林百合, 仁科幸子, 東範行
  • Organizer: 第35回日本小児眼科学会
  • Place of Presentation: 東京
• [Presentation] 新生児におけるトスフロキサシン点眼液の有効性と安全性の検討 (2010)
  • Author(s): 宮永嘉隆, 東範行, 大野重昭
  • Organizer: 第64回日本臨床眼科学会
  • Place of Presentation: 神戸