Activities of cerebellar neurons in behaving mice

• Project/Area Number: 22680031
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Neurophysiology and muscle physiology
• Research Institution: The University of Tokyo
• Principal Investigator: KITAMURA Kazuo 東京大学, 大学院・医学系研究科, 准教授 (60423159)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥25,220,000 (Direct Cost: ¥19,400,000、Indirect Cost: ¥5,820,000); Fiscal Year 2012: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2011: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000); Fiscal Year 2010: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
• Keywords: 神経科学 / 生理学 / パッチクランプ / 光子イメージング / ２光子イメージング / 運動 / 2光子イメージング

Research Abstract

To elucidate the mechanism of cerebellar dependent motor control and motor learning, we have developed an efficient motor task apparatus for mice to perform licking and mechanical lever-pushing under head-fixed configuration. Furthermore, we have shown that synchrony of climbing fiber responses in Purkinje cells correlate with the longitudinal stripes of cerebellar cortex at single cell level.

Research Products

• [Journal Article] Spatiotemporal Dynamics of Functional Clusters of Neurons in the Mouse Motor Cortex during a Voluntary Movement. (2013)
  • Author(s): Hira, R., Ohkubo, F., Ozawa, K., Isomura, Y., Kitamura, K., Kano, M., Kasai, H. & Matsuzaki, M.
  • Journal Title: J. Neurosci Volume: 33 Pages: 1377-1390
  • Peer Reviewed
• [Journal Article] Calcium-dependent regulation of climbing fiber synapse elimination during postnatal cerebellar development. (2013)
  • Author(s): Kano, M., Nakayama, H., Hashimoto, K., Kitamura, K., Sakimura, K. & Watanabe, M.
  • Journal Title: J. Physiol. Volume: -
  • Peer Reviewed
• [Journal Article] Spatiolemporal dynamics of functional clusters of neurons in mouse motor corlex during a voluntary movement (2013)
  • Author(s): Hira R, Okubo F, Ozawa K, Isomura Y, KHamura K, Kano M, Kasai H, Matsuzaki M
  • Journal Title: Journal of Neuroscience Volume: 33 Issue: 4 Pages: 1377-90
  • DOI: 10.1523/jneurosci.2550-12.2013
  • Peer Reviewed
• [Journal Article] Dendritic calciumsignaling in cerebellar Purkinje cell (2012)
  • Author(s): Kitamura, K. & Kano, M.
  • Journal Title: Neural Networks published online
  • Peer Reviewed
• [Journal Article] GABAergic inhibition regulates developmental synapse elimination in the cerebellum. (2012)
  • Author(s): Nakayama, H., Miyazaki, T., Kitamura, K., Hashimoto, K., Yanagawa, Y., Obata, K., Sakimura, K., Watanabe, M. & Kano, M.
  • Journal Title: Neuron Volume: 74 Pages: 384-396
  • Peer Reviewed
• [Journal Article] Matsuki, N. & Ikegaya, Y. Locally synchronized synaptic inputs. (2012)
  • Author(s): Takahashi, N., Kitamura, K., Matsuo, N., Mayford, M., Kano, M.
  • Journal Title: Science Volume: 335 Pages: 353-356
  • Peer Reviewed
• [Journal Article] 小脳 プルキンエ細胞樹状突起活動の in vivo イメージング (2012)
  • Author(s): 喜多村和郎,橋本浩一,狩野方伸
  • Journal Title: 生体の科学 Volume: 63 Pages: 26-33
• [Journal Article] Dendritic calcium signaling in cerebellar Purkinje cell. (2012)
  • Author(s): Kitamura K
  • Journal Title: Neural Networks Volume: - Pages: 11-7
  • DOI: 10.1016/j.neunet.2012.08.001
  • Peer Reviewed
• [Journal Article] Locally synchronized synaptic inputs (2012)
  • Author(s): Takahashi N, Kitamura K, Matsuo N, Mayford M, Kano M, Matsuki N, Ikegaya Y
  • Journal Title: Science Volume: 335 Issue: 6066 Pages: 353-356
  • DOI: 10.1126/science.1210362
  • Peer Reviewed
• [Journal Article] 小脳プルキンエ細胞樹状突起活動のin vivoイメージング (2012)
  • Author(s): 喜多村和郎, 橋本浩一, 狩野方伸
  • Journal Title: 生体の科学 Volume: 63 Pages: 26-33
• [Journal Article] Dendritic calcium signaling triggered by spontaneous and sensory-evoked climbing fiber input to cerebellar Purkinje cells in vivo. (2011)
  • Author(s): Kitamura, K. & Hausser, M.
  • Journal Title: J. Neurosci.31 Pages: 10847-10858
  • Peer Reviewed
• [Journal Article] Postsynaptic P/Q-type Ca2+ channel in Purkinje cell mediates synaptic competition and elimination in developing cerebellum. (2011)
  • Author(s): Hashimoto, K., Tsujita, M.Miyazaki, T., Kitamura, K., Yamazaki, M., Shin, H.-S., Watanabe, M., Sakimura, K. & Kano, M.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108 Pages: 2447-2457
  • Peer Reviewed
• [Journal Article] Invivo two-photonuncagingofglutamaterevealingthestructure-functionrelationshipsofdendriticspinesintheneocortexofadultmice. (2011)
  • Author(s): Noguchi,J.,Nagaoka,A.,Watanabe,S.,Ellis-Davies,G.C.,Kitamura,K.,Kano,M.,Matsuzaki,M.&Kasai,H.
  • Journal Title: J.Physiol Volume: 589 Pages: 2447-2457
  • Peer Reviewed
• [Journal Article] Dendritic calcium signaling triggered by spontaneous and sensory-evoked climbing fiber input to cerebellar Purkinje cells in vivo (2011)
  • Author(s): Kitamura, K. & Hausser, M
  • Journal Title: J. Neurosci Volume: 31 Issue: 30 Pages: 10847-10858
  • DOI: 10.1523/jneurosci.2525-10.2011
  • Peer Reviewed
• [Journal Article] Postsynaptic P/Q-type Ca(2+) channel in Purkinje cell mediates synaptic competition and elimination in developing cerebellum (2011)
  • Author(s): Hashimoto, K.; Tsujita, M.; Miyazaki, T., et al
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 108(24) Issue: 24 Pages: 9987-9992
  • DOI: 10.1073/pnas.1101488108
  • Peer Reviewed
• [Journal Article] In vivo two-photon uncaging of glutamate revealing the structure-function relationships o f dendritic spines in the neocortex of adult mice. (2011)
  • Author(s): Noguchi, J., Nagaoka, A., Watanabe, S., Ellis-Davies, G.C.R., Kitamura, K., Kano, M., Matsuzaki, M. & Kasai H.
  • Journal Title: J.Physiol. Volume: 589 Issue: 10 Pages: 2320-2329
  • DOI: 10.1113/jphysiol.2011.207100
  • Peer Reviewed
• [Journal Article] Calcium-dependentregulationofclimbingfibersynapseeliminationduringpostnatalcerebellardevelopment.
  • Author(s): Kano,M.,Nakayama,H.,Hashimoto,K.,Kitamura,K.,Sakimura,K.&Watanabe,M.
  • Journal Title: J. Physiol.inpress
  • Peer Reviewed
• [Presentation] マウスバレル皮質における感覚シナプス入力の可視化 (2013)
  • Author(s): 喜多村和郎、狩野方伸
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] マウス小脳における登上線維応答の同期性とAldolaseC 発現パ ターンとの関連 (2013)
  • Author(s): 堤新一郎、多田真弓、崎村建司、喜多村 和郎、狩野方伸
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] Climbing fiber network refinement and desynchronization of Purkinje cell population activity during postnatal cerebellar development. (2013)
  • Author(s): Jean-Marc Good, Kazuo Kitamura, Kenji Sakimura, Masanobu Kano.
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] マウスバレル皮質における感覚シナプス入力の可視化 (2013)
  • Author(s): 喜多村和郎、狩野方伸
  • Organizer: 第９０回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] マウス小脳における登上線維応答の同期性とAldolaseC発現パターンとの関連 (2013)
  • Author(s): 堤新一郎、多田真弓、崎村建司、喜多村和郎、狩野方伸
  • Organizer: 第９０回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] Climbing fiber network refinement and desynchronization of Purkinje cell population activity during postnatal cerebellar development. (2013)
  • Author(s): Jean-Marc Good, Kazuo Kitamura, Kenji Sakimura, Masanobu Kano
  • Organizer: 第９０回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] Visualization of an active ensemble of cortical neurons in vivo using a novel activity-dependent promoter E-SARE. (2012)
  • Author(s): T. KAWASHIMA, K. KITAMURA, K. OHKI, K. SUZUKI, M. NONAKA, S. KAMIJO, S. TAKEMOTO-KIMURA, M. KANO, H. OKUNO, H. BITO.
  • Organizer: Neuroscience 2012
  • Place of Presentation: New Orleans, USA.
• [Presentation] Postnatal climbing fiber synapse remodeling desynchronizes population activity of Purkinje cells in developing cerebellum. (2012)
  • Author(s): Jean- Marc Good, Kazuo Kitamura, Kenji Sakimura, Masanobu Kano.
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] 新規活動依存的プロモーターE- SARE を用いた、大脳皮質における機 能的回路の同定. (2012)
  • Author(s): 川島 尚之、喜多村 和郎、大木 研一、 鈴木 敢三、 野中 美応、上條 諭志、竹 本 さやか、狩野 方伸、 奥野 浩行、尾 藤 晴彦.
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] Heterogeneous organization of individual synaptic inputs in the mouse barrel cortex. (2012)
  • Author(s): Kazuo Kitamura, Yuji Ikegaya, Masanobu Kano.
  • Organizer: FENS Forum 2012
  • Place of Presentation: Barcelona, Spain.
• [Presentation] Spatial And Temporal Structure Of Cortical Microcircuit Activity For Generating Voluntary Movement. (2012)
  • Author(s): Hira R., Ohkubo F., Ozawa K., Isomura Y., Kitamura K., Kano M., Kasai H. & Matsuzaki M.
  • Organizer: FENS Forum 2012
  • Place of Presentation: Barcelona, Spain.
• [Presentation] 光子イメージング による単一ニューロン・単一シナプスの 活動計測. (2012)
  • Author(s): 喜多村和郎.生体内 2
  • Organizer: 第2回睡眠研究会
  • Place of Presentation: 名古屋
• [Presentation] 大脳皮質における感覚シナプス入力の可視化. (2012)
  • Author(s): 喜多村和郎.
  • Organizer: 生理研研究会「シナプス可塑性の動作原理?分子から行動で?」
  • Place of Presentation: 岡崎
• [Presentation] Visualization of an active ensemble of cortical neurons in vivo using a novel activity-dependent promoter E-SARE (2012)
  • Author(s): T. KAWASHIMA, K. KITAMURA, K. OHKI, K. SUZUKI, M. NONAKA, S. KAMIJO, S. TAKEMOTO-KIMURA, M. KANO, H. OKUNO, H. BITO
  • Organizer: Neuroscience 2012
  • Place of Presentation: New Orleans
• [Presentation] Postnatal climbing fiber synapse remodeling desynchronizes population activity of Purkinje cells in developing cerebellum (2012)
  • Author(s): Jean-Marc Good, Kazuo Kitamura, Kenji Sakimura, Masanobu Kano
  • Organizer: 第３５回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] 新規活動依存的プロモーターE-SAREを用いた、大脳皮質における機能的回路の同定 (2012)
  • Author(s): 川島 尚之、喜多村 和郎、大木 研一、鈴木 敢三、野中 美応、上條 諭志、竹本 さやか、狩野 方伸、奥野 浩行、尾藤 晴彦
  • Organizer: 第３５回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] Heterogeneous organization of individual synaptic inputs in the mouse barrel cortex. (2012)
  • Author(s): Kazuo Kitamura, Yuji Ikegaya, Masanobu Kano
  • Organizer: FENS Forum 2012
  • Place of Presentation: Barcelona
• [Presentation] Spatial And Temporal Structure Of Cortical Microcircuit Activity For Generating Voluntary Movement (2012)
  • Author(s): Hira R., Ohkubo F., Ozawa K., Isomura Y., Kitamura K., Kano M., Kasai H. & Matsuzaki M.
  • Organizer: FENS Forum 2012
  • Place of Presentation: Barcelona
• [Presentation] 生体内２光子イメージングによる単一ニューロン・単一シナプスの活動計測． (2012)
  • Author(s): 喜多村和郎
  • Organizer: 第２回睡眠研究会
  • Place of Presentation: 名古屋
• [Presentation] 大脳皮質における感覚シナプス入力の可視化． (2012)
  • Author(s): 喜多村和郎
  • Organizer: 生理研研究会「シナプス可塑性の動作原理～分子から行動まで～」
  • Place of Presentation: 岡崎
• [Presentation] Two-photon imaging of neuronal population activities in cerebellar cortex (2011)
  • Author(s): Kazuo Kitamura
  • Organizer: 分子生物学会(ワークショップ)
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-13
• [Presentation] Heterogeneous organization of sensory synaptic inputs in the mouse barrel cortex (2011)
  • Author(s): Kazuo Kitamura
  • Organizer: 生理研国際研究集会
  • Place of Presentation: 岡崎カンファレンスセンター(愛知県)(招待講演)
  • Year and Date: 2011-12-09
• [Presentation] Desynchronization of cerebellar Purkinje cell population activity during postnatal development (2011)
  • Author(s): Jean-Marc Good, Naoki Hidaka, Kazuo Kitamura, Masanobu Kano
  • Organizer: Neuro2011
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-14
• [Presentation] Enhanced synchrony of climbing fiber-induced Ca2+ signaling in cerebellar Purkinje cells caused by elevated synchronous activity of inferior olivary neurons in GluD2 (GluR δ2) knockout mouse (2011)
  • Author(s): Miki Hashizume, Kazuo, Kitamura, Kenji Sakimura, Masanobu Kano
  • Organizer: Neuro2011
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-14
• [Presentation] Two-photon imaging of Purkinje cell dendritic activity in vivo (2011)
  • Author(s): Kazuo Kitamura
  • Organizer: IBRO World Congress
  • Place of Presentation: Florence, Italy(シンポジウム)
  • Year and Date: 2011-07-16
• [Presentation] DendriticactivitiesofcerebellarPurkinjecellinvivo (2011)
  • Author(s): K.Kitamura&M.Hausser.
  • Organizer: 第88回日本生理学大会
  • Year and Date: 2011-03-28
• [Presentation] Dendritic activities of cerebellar Purkinje cell in vivo (2011)
  • Author(s): K.Kitamura, M.Hausser
  • Organizer: 第88回日本生理学大会
  • Place of Presentation: (誌上開催(震災のため))
  • Year and Date: 2011-03-28
• [Presentation] Two-photon imaging of neuronal population activities in cerebellar cortex. (2011)
  • Author(s): Kazuo Kitamura.
  • Organizer: 第34回分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Heterogeneous organization of sensory synaptic inputs in the mouse barrel cortex. (2011)
  • Author(s): Kazuo Kitamura.
  • Organizer: 生理研国際研究集会
  • Place of Presentation: 岡崎
• [Presentation] Enhanced synchrony of climbing fiber-induced Ca2+ signaling in cerebellar Purkinje cells caused by elevated synchronous activity of inferior olivary neurons in GluD2 (GluRδ2) knockout mouse. (2011)
  • Author(s): Miki Hashizume, Kazuo, Kitamura, Kenji Sakimura and Masanobu Kano.
  • Organizer: Neuro2011
  • Place of Presentation: 横浜
• [Presentation] Two-photonimagingofPurkinjecelldendriticactivityinvivo. (2011)
  • Author(s): KazuoKitamura.
  • Organizer: 8thIBROWorldCongress
  • Place of Presentation: Florence,Itary
• [Presentation] 樹状突起活動のin vivoイメージングと光操作 (2010)
  • Author(s): 喜多村和郎
  • Organizer: 第2回光操作研究会
  • Place of Presentation: 岡崎カンファレンスセンター(愛知県岡崎市)(招待講演)
  • Year and Date: 2010-09-09
• [Presentation] 登上線維の発達過程におけるP/Q型電位依存性カルシウムチャネルの役割 (2010)
  • Author(s): 橋本浩一, ら
  • Organizer: Neuro2010(シンポジウム)
  • Place of Presentation: 神戸コンベンションセンター(兵庫県神戸市)
  • Year and Date: 2010-09-04
• [Presentation] GluD2 (GluRδ2)ノックアウトマウスの小脳プルキンエ細胞集団の2光子カルシウムイメージング解析 (2010)
  • Author(s): 橋爪幹, ら
  • Organizer: Neuro2010
  • Place of Presentation: 神戸コンベンションセンター(兵庫県神戸市)
  • Year and Date: 2010-09-02
• [Presentation] Simultaneous two-photon calcium imaging and patch-clamp recordings in vivo (2010)
  • Author(s): K.Kitamura, M.Hausser, M.Kano
  • Organizer: Jacques Monod Conference "Imaging brain circuits in health and disease"
  • Place of Presentation: Biological Station, CNRS (Roscoff, France)
  • Year and Date: 2010-07-01
• [Presentation] 生体内における神経活動の2光子イメージング (2010)
  • Author(s): 喜多村和郎
  • Organizer: 第5回日本分子イメージング学会総会・学術集会
  • Place of Presentation: ピアザ淡海(滋賀県大津市)(招待講演)
  • Year and Date: 2010-05-22
• [Presentation] 小脳登上線維-プルキンエ細胞シナプスの生後発達におけるP/Q型電位依存性カルシウムチャネルの役割 (2010)
  • Author(s): 橋本浩一, ら
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市民文化ホール(岩手県盛岡市)
  • Year and Date: 2010-05-19
• [Presentation] 樹状突起活動の in vivo イメージングと光操作. (2010)
  • Author(s): 喜多村和郎.
  • Organizer: 第2回光操作研究会
  • Place of Presentation: 岡崎
• [Presentation] 登上線維の発達過程における P/Q 型電位依存性カルシウムチャネルの役割 (2010)
  • Author(s): 橋本浩一、辻田実加、喜多村和郎、宮崎 太輔、山崎真弥、Hee-Sup Shin、渡辺雅 彦、崎村建司、狩野方伸.
  • Organizer: Neuro2010
  • Place of Presentation: 神戸
• [Presentation] GluD2(GluRδ2)ノックアウトマウスの小脳プルキンエ細胞集団の2 光子カルシウムイメージング解析. (2010)
  • Author(s): 橋爪幹、喜多村和郎、崎村建司、狩野方伸
  • Organizer: Neuro2010
  • Place of Presentation: 神戸
• [Presentation] Simultaneous two-photon calcium imaging and patch-clamp recordings in vivo (2010)
  • Author(s): K. Kitamura, M. Hausser & M. Kano
  • Organizer: Jacques Monod Conference"Imaging brain circuits in health and disease
  • Place of Presentation: Roscoff, France
• [Presentation] 生体内における神経活動 の2光子イメージング (2010)
  • Author(s): 喜多村和郎
  • Organizer: 第5回日本分子 イメージング学会総会・学術集会
  • Place of Presentation: 大津
• [Presentation] 小脳登上線維?プルキンエ細胞シナプスの生後発達に おける P/Q 型電位依存性カルシウムチャ ネルの役割. (2010)
  • Author(s): 橋本浩一、辻田実加、宮崎太輔、山崎真 弥、喜多村和郎、Hee-Sup Shin、渡辺雅 彦、崎村建司、狩野方伸.
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡
• [Book] 脳神経科学イラストレイテッド (2013)
  • Author(s): 喜多村和郎.
  • Publisher: invivoイメージング羊土社
• [Book] 最新パッチクランプ実験 技術法,岡田泰伸 編,吉岡書店 (2013)
  • Author(s): 喜多村和郎.
  • Publisher: in vivo イメージングパッチ法
• [Book] 脳神経科学イラストレイテッド (2013)
  • Author(s): 喜多村和郎
  • Total Pages: 8
  • Publisher: 羊土社
• [Book] Patch Clamp Techniques (Y. Okada ed.), Springer (2012)
  • Author(s): Kazuo Kitamura.
  • Publisher: Two-photon targeted patch-clamp recordings in vivo
• [Book] Patch Clamp Techniques (2012)
  • Author(s): Kazuo Kitamura
  • Total Pages: 11
  • Publisher: Springer
• [Book] 第12章in vivoイメージングパッチ法(最新パッチクランプ実験技術法、岡田泰伸編) (2011)
  • Author(s): 喜多村和郎
  • Total Pages: 8
  • Publisher: 吉岡書店