Development of Novel Fluorogenic Probes for Labeling Intracellular Proteins
| Project/Area Number |
22685016
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Osaka University |
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Principal Investigator |
HORI Yuichiro 大阪大学, 工学(系)研究科(研究院), 助教 (00444563)
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| Project Period (FY) |
2010-04-01 – 2013-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000)
Fiscal Year 2012: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2011: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2010: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Keywords | 蛋白質標識 / 生細胞蛍光イメージング / 発蛍光プローブ / PYPタグ / タグ蛋白質 / 無洗浄イメージング / 蛋白質標識法 / 蛍光プローブ / PYP / 蛍光強度増大 / 桂皮酸誘導体 / クマリン誘導体 |
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| Research Abstract |
Fluorescence protein labeling by a specific pair of a protein tag and synthetic probe is a powerful chemistry-based approach to investigate protein function and localization inside living cells. In this research, we developed a protein labeling system based on fluorogenic probes and photoactive yellow protein (PYP) tag. By utilizing this technique, we were succeeded in specific imaging of proteins inside living cells with high S/N ratio and excellent labeling kinetics. Furthermore, the labeling technique was applied to the imaging of DNA methylation.
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Report
(4 results)
Research Products
(31 results)
