| Budget Amount *help |
¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000)
Fiscal Year 2012: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2011: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2010: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Research Abstract |
Fluorescence protein labeling by a specific pair of a protein tag and synthetic probe is a powerful chemistry-based approach to investigate protein function and localization inside living cells. In this research, we developed a protein labeling system based on fluorogenic probes and photoactive yellow protein (PYP) tag. By utilizing this technique, we were succeeded in specific imaging of proteins inside living cells with high S/N ratio and excellent labeling kinetics. Furthermore, the labeling technique was applied to the imaging of DNA methylation.
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