Development and application of a novel capture method for living uncultured microorganisms.

• Project/Area Number: 22686050
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Civil and environmental engineering
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: SEKIGUCHI Yuji 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 研究グループ長 (20313570)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000); Fiscal Year 2012: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000); Fiscal Year 2011: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000); Fiscal Year 2010: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
• Keywords: 環境技術 / 微生物 / 土木環境システム / 水質汚濁 / 土壌汚染防止 / 浄化 / 水質汚濁・土壌汚染防止・浄化 / 生態学

Research Abstract

Gemmatimonas aurantiaca was used as one of the model fastidious microbes, and was subjected to FISH detection, cell collection with flow cytometry, and subsequent selection of peptides that specifically bind to cells of G. aurantiaca, resulting in the suc

Research Products

• [Journal Article] Oligosphaera ethanolica gen. nov., sp. nov., an anaerobic, carbohydrate-fermenting bacterium isolated from methanogenic sludge, and description of Oligosphaeria classis nov. in the phylum Lentisphaerae (2013)
  • Author(s): 邱 艶 玲 、 関 口 勇 地
  • Journal Title: Int J Syst Evol Microbiol. Volume: 63 Pages: 533-539
  • Peer Reviewed
• [Journal Article] Quantitative detection of previously characterized syntrophic bacteria in anaerobicwastewater treatment systems by sequence-specific rRNA cleavage method (2012)
  • Author(s): 成廣隆、 関口勇地
  • Journal Title: Water Research Volume: 46 Pages: 2167-2175
  • Peer Reviewed
• [Journal Article] Quantitative detection of previously characterized syntrophic bacteria in anaerobic wastewater treatment systems by sequence-specific rRNA cleavage method (2012)
  • Author(s): 成廣隆
  • Journal Title: WATER RESEARCH Volume: 46 Issue: 7 Pages: 2167-2175
  • DOI: 10.1016/j.watres.2012.01.034
  • Peer Reviewed
• [Journal Article] Oligosphaera ethanolica gen.nov., sp.nov., an anaerobic, carbohydrate-fermenting bacterium isolated from methanogenie sludge, and description of the Oligosphaeria classis nov.in the bacterial phylum Lentisphaerae (2012)
  • Author(s): 邱艶麗、関口勇地
  • Journal Title: Int J Syst Evol Microbiol Volume: (in-press) Issue: Pt_2 Pages: 533-539
  • DOI: 10.1099/ijs.0.039545-0
  • Peer Reviewed
• [Journal Article] Oligonucleotide probes for environmental monitoring of methanogenic archaea (2011)
  • Author(s): 成 廣隆、 関口勇 地
  • Journal Title: Microbiol Biotechnology Volume: 4 Pages: 585-602
  • Peer Reviewed
• [Presentation] Cultivation and characterization of anaerobes relevant to sludge bulking in methanogenic wastewater treatment system (2012)
  • Author(s): 黒田恭平、関口勇地
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 豊橋技術科学大学(愛知県)
  • Year and Date: 2012-09-20
• [Presentation] 嫌気性廃水処理汚泥のバルキングに関与する複合微生物群の分子遺伝学的解析および培養 (2011)
  • Author(s): 黒田恭平、関口勇地
  • Organizer: 土木学会新潟会研究調査発表会
  • Place of Presentation: ハイブ長岡(新潟県)
  • Year and Date: 2011-11-22
• [Presentation] 嫌気性廃水処理汚泥のバルキングに関与する複合微生物群の分子遺伝学的解析および培養 (2011)
  • Author(s): 黒田恭平、関口勇地
  • Organizer: 土木学会新潟会研究調査発表会
  • Place of Presentation: 長岡市(ハイブ長岡)
  • Year and Date: 2011-11-22
• [Presentation] 下水処理 UASB プロセスの汚泥内微生物群集構造解析と高頻度に検出される未培養微生物群の検出 (2011)
  • Author(s): 井口晃徳、 関口勇地
  • Organizer: 日本微生物生態学会大会
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2011-10-08
• [Presentation] 下水処理UASBプロセスの汚泥内微生物群集構造解析と高頻度に検出される未培養微生物群の検出 (2011)
  • Author(s): 井口晃徳、関口勇地
  • Organizer: 日本微生物生態学会大会
  • Place of Presentation: 京都(京都大学)
  • Year and Date: 2011-10-08
• [Presentation] Ecophysiology of formerly uncultured fastidious Chloroflexi and Lentisphaerae in natural and biotechnological systems (2011)
  • Author(s): 関口勇地
  • Organizer: International Union of Microbiological Societies 2011
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-06
• [Presentation] Ecophysiology of formerly uncultured fastidious Chloroflexi and Lentisphaerae in natural and biotechnological systems (2011)
  • Author(s): 関口勇地
  • Organizer: International Union of Microbiological Societies 2011
  • Place of Presentation: 札幌(札幌コンベンションセンター)(招待講演)
  • Year and Date: 2011-09-06
• [Presentation] Cultivation and characterization of anaerobes relevant to sludge bulking in methanogenic wastewater treatment system
  • Author(s): 黒田恭平
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 豊橋技術科学大学
  • Invited