Genetic basis of novelty generation by symbiosis

• Project/Area Number: 22687018
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: SHIGENOBU Shuji 基礎生物学研究所, 生物機能解析センター, 特任准教授 (30399555)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥25,740,000 (Direct Cost: ¥19,800,000、Indirect Cost: ¥5,940,000); Fiscal Year 2012: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2011: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2010: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
• Keywords: 進化発生 / 共生 / 次世代シーケンサー / RNA-seq / アブラムシ / Buchnera / 根粒菌 / トランスクリプトーム / 次世代シークエンサー

Research Abstract

The objective of this study is to decipher genetic basis of novelty generation by symbiosis using aphid-Buchnera endosymbiosis as a model system. Taking an advantage of new technologies such as next-generation sequencing, we discovered novel secreted peptides that were preferentially expressed in the symbiotic organ of the host insects. The novel secreted peptides were designated BCR family. We also found that a member of BCR affect the growth of bacteria. These data suggest that aphids evolved novel secreted proteins for symbiosis with bacterial endosymbiont

Research Products

• [Journal Article] Aphids evolved novel secreted proteins for symbiosis with bacterial endosymbiont (2013)
  • Author(s): Shigenobu, S. and Stern, D
  • Journal Title: Proc. Royal Soc. B Volume: 280 Issue: 1750 Pages: 20121952-20121952
  • DOI: 10.1098/rspb.2012.1952
  • Peer Reviewed
• [Journal Article] Maintenance of essential amino acid synthesis pathways in the Blattabacterium cuenoti symbiont of a wood-feeding cockroach (2013)
  • Author(s): Tokuda, G., Elbourne, L.D.H., Kinjo, Y., Saitoh, S.,Sabree, Z., Hojo, M., Yamada, A., Hayashi, Y.Shigenobu,S., Bandi, C., et al
  • Journal Title: Biol. Lett Volume: 9 Issue: 3 Pages: 20121153-20121153
  • DOI: 10.1098/rsbl.2012.1153
  • Peer Reviewed
• [Journal Article] Exploration and characterization of genes involved in the synthesis of diterpene defence secretion in nasute termite soldiers. (2012)
  • Author(s): Hojo M, Maekawa K, Saitoh S, Shigenobu S, Miura T, Hayashi Y, Tokuda G, Maekawa H
  • Journal Title: Insect Mol Biol Volume: 21 Issue: 6 Pages: 545-557
  • DOI: 10.1111/j.1365-2583.2012.01162.x
  • Peer Reviewed
• [Journal Article] Sexual and asexual oogenesis require the expression of unique and shared sets of genes in the insect Acyrthosiphon pisum (2012)
  • Author(s): Gallot, A., Shigenobu, S., Hashiyama, T., Jaubert-Possamai, S. & Tagu, D
  • Journal Title: BMC Genomics Volume: 13 Issue: 1 Pages: 76-76
  • DOI: 10.1186/1471-2164-13-76
  • Peer Reviewed
• [Journal Article] Genomic revelations of a mutualism: the pea aphid and its obligate bacterial symbiont (2011)
  • Author(s): Shigenobu, S. and Wilson, A. C.
  • Journal Title: Cellular and Molecular Life Sciences Volume: 68 Issue: 8 Pages: 1297-1297
  • DOI: 10.1007/s00018-011-0645-2
  • Peer Reviewed
• [Journal Article] Genome expansion and differential expression of amino acid transporters at the aphid/Buchnera symbiotic interface (2011)
  • Author(s): Price, D. R., Duncan, R. P., Shigenobu, S. and *Wilson, A. C.
  • Journal Title: Molecular Biology and Evolution Volume: 28 Issue: 11 Pages: 3113-3126
  • DOI: 10.1093/molbev/msr140
  • Peer Reviewed
• [Presentation] Aphids evolved novel secreted proteins for symbiosis with bacterial endosymbiont (2013)
  • Author(s): 重信秀治
  • Organizer: 第57回日本応用動物昆虫学会大会
  • Place of Presentation: 日本大学湘南キャンパス(神奈川県藤沢市)
• [Presentation] Aphids evolved novel secreted proteins for symbiosis with bacterial endosymbiont (2013)
  • Author(s): 重信秀治
  • Organizer: 第57回日本応用動物昆虫学会大会
  • Place of Presentation: 日本大学湘南キャンパス（神奈川県藤沢市）
• [Presentation] Aphid bactreiocytes a model systemto study evolutionary and developmental novelty through symbiosis (2012)
  • Author(s): Shuji Shigenobu
  • Organizer: 2012 APDBC Satellite Meeting on EvoDevo (招待講演)
  • Place of Presentation: Taiwan
  • Year and Date: 2012-10-05
• [Presentation] 次世代シークエンシング技術と非モデル生物がもたらす分子生物学の未来 (2012)
  • Author(s): 重信秀治
  • Organizer: 第35回日本分子生物学会年会(招待講演)
  • Place of Presentation: 福岡国際会議場(福岡県福岡市)
• [Presentation] 次世代シークエンシング時代のアブラムシ研究戦略 (2012)
  • Author(s): 重信秀治
  • Organizer: 日本アブラムシ研究会第2回研究集会
  • Place of Presentation: 岡崎コンファレンスセンター(愛知県岡崎市)
• [Presentation] 共生のゲノム科学:アブラムシ細胞内共生系をモデルとして (2012)
  • Author(s): 重信秀治
  • Organizer: 植物微生物研究会第22回研究交流会(招待講演)
  • Place of Presentation: 神戸大学(兵庫県神戸市)
• [Presentation] 次世代シークエンシング技術と非モデル生物がもたらす分子生物学の未来 (2012)
  • Author(s): 重信秀治
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Invited
• [Presentation] Aphid bactreiocytes, a model system to study evolutionary and developmental novelty through symbiosis (2012)
  • Author(s): Shuji Shigenobu
  • Organizer: 2012 APDBC Satellite Meeting on EvoDevo
  • Place of Presentation: Taiwan
  • Invited
• [Presentation] 次世代シークエンシング時代のアブラムシ研究戦略 (2012)
  • Author(s): 重信秀治
  • Organizer: 日本アブラムシ研究会第2回研究集会
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県岡崎市）
• [Presentation] 共生のゲノム科学：アブラムシ細胞内共生系をモデルとして (2012)
  • Author(s): 重信秀治
  • Organizer: 植物微生物研究会第 22 回研究交流会
  • Place of Presentation: 神戸大学（兵庫県神戸市）
  • Invited
• [Presentation] Convergent evolution of novel secreted proteins in the aphid- acterium symbiosis and plant-bacterium symbiosis. (2011)
  • Author(s): 重信秀治
  • Organizer: 日本新か学会第13回大会
  • Place of Presentation: 京都大学(京都)
• [Presentation] Convergent Evolution of Novel Secreted Proteins in the Aphid-bacterium Symbiosis (2011)
  • Author(s): 重信秀治
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川市大雪クリスタルホール(北海道)
• [Presentation] Convergent evolution of novel secreted proteins in the aphid-bacterium symbiosis and plant-bacterium symbiosis (2011)
  • Author(s): 重信秀治
  • Organizer: 日本進化学会第13回大会
  • Place of Presentation: 京都大学(京都府)
• [Presentation] The pea aphid, Acyrthosiphon pisum: an emerging model organism for ecological, developmental and evolutionary studies (2010)
  • Author(s): 重信秀治
  • Organizer: 日本進化学会第12回大会
  • Place of Presentation: 東京工業大学(東京)
• [Presentation] The pea aphid, Acyrthosiphon pisum: an emerging model organism for ecological, developmental and evolutionary studies (2010)
  • Author(s): 重信秀治
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京大学(東京)
• [Presentation] Genomic revelations of a mutualism: the pea aphid and its obligatebacterial symbiont (2010)
  • Author(s): Shuji Shigenobu
  • Organizer: 国際生物学賞記念シンポジウム
  • Place of Presentation: つくば国際会議場(茨城)
• [Presentation] The pea aphid, Acyrthosiphon pisum : an emerging model organism for ecological, developmental and evolutionary studies (2010)
  • Author(s): 重信秀治
  • Organizer: 日本進化学会第12回大会
  • Place of Presentation: 東京工業大学(東京)
• [Presentation] The pea aphid, Acyrthosiphon pisum : an emerging model organism for ecological, developmental and evolutionary studies (2010)
  • Author(s): 重信秀治
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京大学(東京)
• [Presentation] Genomic revelations of a mutualism : the pea aphid and its obligate bacterial symbiont (2010)
  • Author(s): Shuji Shigenobu
  • Organizer: 国際生物学賞記念シンポジウム
  • Place of Presentation: つくば国際会議場(茨城)
• [Book] 2012年「共生なくしてわれわれはなかった」地球外生命9の論点(立花隆、佐藤勝彦ほか共著) (2012)
  • Author(s): 重信秀治
  • Publisher: 講談社(東京)
• [Remarks] ホームページ
  • URL: http://www.nibb.ac.jp/sections/nibb_core_research_facilities/shigenobu/
• [Remarks] プレスリリース：アブラムシと細菌が共生する細胞ではたらく新しい遺伝子ファミリーを発見
  • URL: http://www.nibb.ac.jp/press/2012/11/22.html