| Project/Area Number |
22700367
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Kyoto University |
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Principal Investigator |
HIOKI Hiroyuki 京都大学, 大学院・医学研究科, 助教 (00402850)
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 神経回路網 / レンチウイルス / 経シナプストレーサー / Tet-Offシステム / 共発現 / 中枢神経系 / 大脳皮質 / 視床 / 局所回路 |
|---|
| Research Abstract |
By using" Double Lentiviral Vector Tet-Off Platform(Hioki et al., 2009),"we developed dual expression system with bidirectional TRE-tight promoter(TREB), internal ribosomal entry site(IRES) derived from encephalomyocarditis virus or 2Asequence of foot and mouth disease virus. We compared the expression level of transgenes in vivo, and revealed that 2A sequence was suitable for dual expression.
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