Quantitative analysis of reciprocal onnection between the thalamus and neocortex by using lentiviral vectors
Project/Area Number |
22700367
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Nerve anatomy/Neuropathology
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Research Institution | Kyoto University |
Principal Investigator |
HIOKI Hiroyuki 京都大学, 大学院・医学研究科, 助教 (00402850)
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Project Period (FY) |
2010 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 神経回路網 / レンチウイルス / 経シナプストレーサー / Tet-Offシステム / 共発現 / 中枢神経系 / 大脳皮質 / 視床 / 局所回路 |
Research Abstract |
By using" Double Lentiviral Vector Tet-Off Platform(Hioki et al., 2009),"we developed dual expression system with bidirectional TRE-tight promoter(TREB), internal ribosomal entry site(IRES) derived from encephalomyocarditis virus or 2Asequence of foot and mouth disease virus. We compared the expression level of transgenes in vivo, and revealed that 2A sequence was suitable for dual expression.
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Report
(3 results)
Research Products
(60 results)
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[Presentation] mDia, a Rho effector and actin nucleator, is critical for growth coneretraction in ephrin-induced axonal repulsion2011
Author(s)
豊田洋輔, 篠原亮太, Dean Thumkeo, 上條博史, 日置寛之, 金子武嗣, 石崎敏理, 古屋敷智之, 成宮周
Organizer
第54回日本神経化学会
Place of Presentation
石川県加賀市瑠璃光
Year and Date
2011-09-26
Related Report
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[Presentation] iPS細胞由来神経細胞におけるAβ産生とセクレターゼ阻害剤に対する応答2011
Author(s)
浅井将, 八幡直樹, 北岡志保, 高橋和利, 浅香勲, 日置寛之, 金子武嗣, 丸山敬, 西道隆臣, 中畑龍俊, 朝田隆, 山中伸弥, 岩田修永, 井上治久
Organizer
第16回日本病態プロテアーゼ学会
Place of Presentation
豊中千里ライフサイエンスセンター
Year and Date
2011-08-26
Related Report
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[Presentation] 皮質視床投射神経細胞への皮質内入力に現れるサブカラム構造2011
Author(s)
田中康裕, 田中康代, 今野美知輝, 藤山文乃, 岡本-古田敬子, 薗村貴弘, 亀田浩司, 日置寛之, 古田貴寛, 中村公一, 金子武嗣
Organizer
第116回 日本解剖学会・全国学術集会
Place of Presentation
(東日本大震災のため、紙上開催)
Year and Date
2011-03-28
Related Report
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[Presentation] Subcolumnar structures in local inputs of pyramidal neurons onto corticothalamic neurons in rat barrel cortex.2010
Author(s)
Tanaka Y, Tanaka Y, Konno M, Fujiyama F, Okamoto-Furuta K, Sonomura T, Kameda H, Hioki H, Furuta T, Nakamura KC, Kaneko T.
Organizer
The 40th Society for Neuroscience Annual Meeting
Place of Presentation
San Diego
Year and Date
2010-11-26
Related Report
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[Presentation] Subcolumnar structures in local inputs of pyramidalneurons onto corticothalamic neurons in rat barrel cortex2010
Author(s)
Tanaka Y, Tanaka Y, Konno M, Fujiyama F, Okamoto-Furuta K, Sonomura T, Kameda H, Hioki H, Furuta T, Nakamura KC, Kaneko T.
Organizer
40th Society for Neuroscience Annual Meeting
Place of Presentation
San Diego
Year and Date
2010-11-16
Related Report
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[Presentation] LENTIVIRAL VECTORS DRIVE HIGH-LEVEL TRANSGENE EXPRESSION IN MARMOSET BRAINS2010
Author(s)
Watakabe A, Takaji M, Nakagami Y, Hioki H, Kaneko T, Kato S, Koboyashi K, Kawashima T, Okuno H, Bito H, Kitamura Y, Yamamori T
Organizer
40th Society for Neuroscience Annual Meeting
Place of Presentation
San Diego
Year and Date
2010-11-16
Related Report
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[Presentation] "TET-OFF" LENTIVIRAL VECTORS DRIVE HIGH-LEVEL TRANSGENE EXPRESSION IN MARMOSET BRAINS.2010
Author(s)
Watakabe A, Takaji M, Nakagami Y, Hioki H, Kaneko T, Kato S, Koboyashi K, Kawashima T, Okuno H, Bito H, Kitamura Y, Yamamori T.
Organizer
The 40th Society for Neuroscience Annual Meeting
Place of Presentation
San Diego
Year and Date
2010-11-16
Related Report
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