The molecular basis of interactions between cancer cells and neurons in glioma invasion
Project/Area Number |
22700375
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Nerve anatomy/Neuropathology
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Research Institution | The University of Tokyo |
Principal Investigator |
SHUO Takuya 東京大学, 医科学研究所, 特任研究員 (90399006)
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Project Period (FY) |
2010 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 脳腫瘍 / 神経膠腫細胞 / 神経細胞 / メタロプロテアーゼ / プロテオグリカン / 糖鎖 / 質量分析 / MALDI(マトリックス支援レーザー脱離イオン化法) / MALDI |
Research Abstract |
To understand the molecular basis of interactions between glioma and neurons, we investigated the role of MT-MMP/neuroglycan C axis. In this study, we found that a recombinant MT-MMP catalytic domain could actually cleave full-length neuroglycan C in two fragments. In addition, we developed a new MALDI-mass spectrometric technique to analyze glycopeptides in a highly sensitive manner, and then we used the method to evaluate glycosylation status of MT-MMP.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] ZF21 protein, a regulator of the disassembly of focal adhesions and cancer metastasis, contains a novel noncanonical pleckstrin homology domain2011
Author(s)
Nagano M, Hoshino D, Koshiba S, Shuo T Koshikawa N, Tomizawa T, Hayashi F, Tochio N, Harada T, Akizawa T Watanabe S, Handa N, Shirouzu M, Kigawa T, Yokoyama S, Seiki M
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Journal Title
J Biol Chem.
Volume: 286(36)
Issue: 36
Pages: 31598-609
DOI
Related Report
Peer Reviewed
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[Presentation] Glycan characterization of MT1-MMP2011
Author(s)
Takuya Shuo
Organizer
an invasion-promoting metalloproteinase, by MALDI-digital ion trap mass spectrometrySeventh General Meeting of the International Proteolysis Society
Place of Presentation
Hilton San Diego Resort and Spa(San Diego/USA)
Year and Date
2011-10-19
Related Report
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