The molecular basis of interactions between cancer cells and neurons in glioma invasion

• Project/Area Number: 22700375
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: The University of Tokyo
• Principal Investigator: SHUO Takuya 東京大学, 医科学研究所, 特任研究員 (90399006)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 脳腫瘍 / 神経膠腫細胞 / 神経細胞 / メタロプロテアーゼ / プロテオグリカン / 糖鎖 / 質量分析 / MALDI(マトリックス支援レーザー脱離イオン化法) / MALDI

Research Abstract

To understand the molecular basis of interactions between glioma and neurons, we investigated the role of MT-MMP/neuroglycan C axis. In this study, we found that a recombinant MT-MMP catalytic domain could actually cleave full-length neuroglycan C in two fragments. In addition, we developed a new MALDI-mass spectrometric technique to analyze glycopeptides in a highly sensitive manner, and then we used the method to evaluate glycosylation status of MT-MMP.

Research Products

• [Journal Article] Detection of the heterogeneous O-glycosylation profile of MT1-MMPexpressed in cancer cells by a simple MALDI-MS method. (2012)
  • Author(s): Shuo T, Koshikawa N, Hoshino D, Minegishi T, Ao-Kondo H, Oyama M, Sekiya S, Iwamoto S, Tanaka K, Seiki M.
  • Journal Title: PLoS One. Volume: 7(8) Issue: 8 Pages: e43751-e43751
  • DOI: 10.1371/journal.pone.0043751
  • Peer Reviewed
• [Journal Article] ZF21 protein, a regulator of the disassembly of focal adhesions and cancer metastasis, contains a novel noncanonical pleckstrin homology domain (2011)
  • Author(s): Nagano M, Hoshino D, Koshiba S, Shuo T Koshikawa N, Tomizawa T, Hayashi F, Tochio N, Harada T, Akizawa T Watanabe S, Handa N, Shirouzu M, Kigawa T, Yokoyama S, Seiki M
  • Journal Title: J Biol Chem. Volume: 286(36) Issue: 36 Pages: 31598-609
  • DOI: 10.1074/jbc.m110.199430
  • Peer Reviewed
• [Presentation] 液体マトリックス3AQ/CHCAを用いた新規MALDI質量分析法による膜型プロテアーゼMT1-MMPのO-結合型糖鎖修飾の解析 (2012)
  • Author(s): 周尾卓也
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡(福岡県福岡市)
  • Year and Date: 2012-12-15
• [Presentation] Detection of the heterogeneous (2012)
  • Author(s): Takuya Shuo
  • Organizer: O-glycosylation of MT1-MMP expressed in cancer cells by a simple MALDI-MS method19th International Mass SpectrometryConference,2
  • Place of Presentation: 国立京都国際会館(京都府京都市)
  • Year and Date: 2012-09-16
• [Presentation] Detection of the heterogeneousO-glycosylation of MT1-MMP expressed in cancer cells by a simple MALDI-MS method (2012)
  • Author(s): 周尾卓也
  • Organizer: 第12回東京大学生命科学シンポジウム
  • Place of Presentation: 東京大学(東京都文京区)
  • Year and Date: 2012-06-30
• [Presentation] Detection of the heterogeneous O-glycosylation profile of MT1-MMP expressed in cancer cells by a simple MALDI-MS method (2012)
  • Author(s): Takuya Shuo
  • Organizer: 19th International Mass Spectrometry Conference
  • Place of Presentation: 国立京都国際会館 京都府京都市
• [Presentation] 液体マトリックス3AQ/CHCAを用いた新規MALDI質量分析法による膜型プロテアーゼMT1-MMPのO-結合型糖鎖修飾の解析 (2012)
  • Author(s): 周尾卓也
  • Organizer: 第８５回 日本生化学会大会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡 福岡県福岡市
• [Presentation] Glycan characterization of MT1-MMP (2011)
  • Author(s): Takuya Shuo
  • Organizer: an invasion-promoting metalloproteinase, by MALDI-digital ion trap mass spectrometrySeventh General Meeting of the International Proteolysis Society
  • Place of Presentation: Hilton San Diego Resort and Spa(San Diego/USA)
  • Year and Date: 2011-10-19
• [Presentation] Glycan characterization of MT1-MMP, an invasion-promoting metalloproteinase, by MALDI-digital ion trap mass spectrometry (2011)
  • Author(s): Takuya Shuo
  • Organizer: Seventh General Meeting of the International Proteolysis Society
  • Place of Presentation: USA/San Diego
  • Year and Date: 2011-10-19
• [Presentation] MALDI質量分析における液体マ トリックス 3-AQ/CHCAを用いた細胞膜貫通型メタロプロテアーゼ MT1-MMP の O-結 合型糖鎖修飾の解析 (2011)
  • Author(s): 周尾卓也
  • Organizer: 第59回質量分析総合討論会
  • Place of Presentation: ホテル阪急エキスポパーク(大阪府吹田市)
  • Year and Date: 2011-09-14
• [Presentation] MALDI質量分析における液体マトリックス3-AQ/CHCAを用いた細胞膜貫通型メタロプロテアーゼMT1-MMPのO-結合型糖鎖修飾の解析 (2011)
  • Author(s): 周尾卓也
  • Organizer: 第59回質量分析総合討論会
  • Place of Presentation: 大阪府吹田市
  • Year and Date: 2011-09-14
• [Presentation] Detection of the heterogeneous O-glycosylation profile of MT1-MMP expressed in cancer cells by a simple MALDI-MS method
  • Author(s): 周尾卓也
  • Organizer: 第１２回 東京大学 生命科学シンポジウム
  • Place of Presentation: 東京大学 東京都文京区
• [Remarks] 東京大学[広報・情報公開]記者発表一覧
  • URL: http://www.u-tokyo.ac.jp/public/public01_240823_j.html
• [Remarks] 東京大学クロールCOE研究成果一覧
  • URL: http://www.u-tokyo.ac.jp.fiw.st/coe/japanese/achievements/category1/base3/report03-03.htm
• [Remarks] 東京大学［広報・情報公開］ 記者発表一覧
  • URL: http://www.u-tokyo.ac.jp/public/public01_240823_j.html
• [Remarks] 東京大学グローバルCOE 研究成果一覧
  • URL: http://www.u-tokyo.ac.jp.fiw.st/coe/japanese/achievements/category1/base3/report03-03.html
• [Remarks] 東京大学医科学研究所 発表論文解説
  • URL: http://www.ims.u-tokyo.ac.jp/imsut/jp/research/papers/post_42.php