| Project/Area Number |
22700421
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Neurophysiology and muscle physiology
|
|---|
| Research Institution | National Institute for Physiological Sciences |
|---|
Principal Investigator |
KOIZUMI Amane 生理学研究所, 細胞器官研究系, 准教授 (10296551)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 脳・神経 / 神経科学 / 生理学 / 脳神経疾患 / バイオテクノロジー |
|---|
| Research Abstract |
The aim of this study is to investigate the function of retinal interneurons, namely amacrine cells, and their dendrites by optogenetic approaches with ectopic expression of photosensitive pigment, melanopsin. We successfully established organotypic tissue culture of adult rodent retina with particle-mediated gene transfer of melanopsin. In addition, we established transgenic mouse line, BitetO-OPN4-mCherry, which express melanopsin(OPN4) as well as mCherry under the presence of tetracycline-activator(tTA).
|
