Development of novel controlling of protozoa without anti-drugs for serving of safety chicken meat

• Project/Area Number: 22700773
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Eating habits, studies on eating habits
• Research Institution: National Agriculture and Food Research Organization (2011-2012); Osaka Yuhigaoka Gakuen College (2010)
• Principal Investigator: MATSUBAYASHI Makoto 独立行政法人農業・食品産業技術総合研究機構, 動物衛生研究所・細菌・寄生虫 研究領域, 主任研究員 (00321076)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 食と環境 / Eimeria tenella / シゾント / トランスクリプトーム / 発育 / 血便 / アイメリア原虫 / 鶏肉 / 原虫酵素 / メロゾイト / Eimeria原虫 / 特異分子 / Apicomplexa

Research Abstract

Chicken coccidiosis is caused by protozoan parasites of Eimeria spp. and mainly characterized as diarrhea. These parasites are responsible for significant economic losses to the worldwide poultry industry. We performed comprehensive gene expression analyses on extracellular sporulation and intestinal developmental stages of E. tenella to understand their biology for the infection. By sequencing of cDNA using the next generation sequencer, assembled contigs were found to contain novel genes of E. tenella. The up- or down- regulated genes at proliferation and differentiation of the second generation schizonts in infected chicken ceca were identified by microarray analysis. These results suggested that the identified genes could be novel targets such as vaccine antigens to control the disease.

Research Products

• [Journal Article] Infectivity of Cryptosporidiumandersoni Kawatabi type relative to thesmall number of oocysts in immunodeficient and immunocompetent neonatal and adult mice (2013)
  • Author(s): Nagano-Koyashiki S, Matsubayashi M(CA), Kimata I, Furuya M, Tani H, Sasai K
  • Journal Title: Parasitol Int.2013 Volume: 62(2) Issue: 2 Pages: 109-11
  • DOI: 10.1016/j.parint.2012.11.001
  • Peer Reviewed
• [Journal Article] Survey on gastrointestinal parasites and detection of Cryptosporidium spp. on cattle in West Java, Indonesia (2013)
  • Author(s): Sylvia Maharani Ananta, Suharno, Adi Hidayat, Makoto Matsubayashi(CA)
  • Journal Title: Asian Pacific Journal of Tropical Medicine Volume: 未定
  • Peer Reviewed
• [Journal Article] Synchronous development of Eimeria tenella in chicken caeca and utility of laser microdissection for purification of single stage schizont RNA (2012)
  • Author(s): Matsubayashi M, Hatta T, Miyoshi T, Anisuzzaman, Alim MA, Yamaji K, Shimura K, Isobe T, Tsuji N
  • Journal Title: Parasitology Volume: 139(12) Issue: 12 Pages: 1553-61
  • DOI: 10.1017/s0031182012001072
  • Peer Reviewed
• [Journal Article] Phylogenetic identification of Cystoisospora spp. from dogs, cats, and raccoon dogs in Japan (2011)
  • Author(s): Matsubayashi M, Carreno RA, Tani H, Yoshiuchi R, Kanai T, Kimata I, Uni S, Furuya M, Sasai K
  • Journal Title: Vet Parasitol Volume: 176(2-3) Issue: 2-3 Pages: 270-4
  • DOI: 10.1016/j.vetpar.2010.11.008
  • Peer Reviewed
• [Journal Article] Effect of low pH on the morphology and viability of Cryptosporidium andersoni sporozoites and histopathology in the stomachs of infected mice (2011)
  • Author(s): Matsubayashi M, Ando H, Kimata I, TakaseH, Nakagawa H, Furuya M, Tani H, Sasai K
  • Journal Title: Int J Parasitol Volume: 41(3-4) Issue: 3-4 Pages: 287-92
  • DOI: 10.1016/j.ijpara.2010.09.009
  • Peer Reviewed
• [Journal Article] Effect of low pH on the morphology and viability of Cryptosporidium andersoni sporozoites and histopathology in the stomach of infected mice (2011)
  • Author(s): Makoto Matsubayashi, et al
  • Journal Title: International Journal for Parasitology Volume: 41 Pages: 287-92
  • Peer Reviewed
• [Journal Article] Phylogenetic identification of Cystoisospora spp.from dogs, cats, and raccoon dogs in Japan (2011)
  • Author(s): Makoto Matsubayashi, et al
  • Journal Title: Veterinary Parasitology Volume: 176 Pages: 270-4
  • Peer Reviewed
• [Journal Article] Morphological changes and viability of Cryptosporidium parvum sporozoites after excystation in cell-free culture media (2010)
  • Author(s): Matsubayashi M, Ando H, Kimata I, Nakagawa H, Furuya M, Tani H, Sasai K
  • Journal Title: Parasitology Volume: 137(13) Issue: 13 Pages: 1861-6
  • DOI: 10.1017/s0031182010000685
  • Peer Reviewed
• [Journal Article] Survey and molecular characterization of Cryptosporidium and Giardia spp. in owned companion animal, dogs and cats, in Japan (2010)
  • Author(s): Yoshiuchi R, Matsubayashi M, Kimata I, Furuya M, Tani H, Sasai K
  • Journal Title: Vet Parasitol Volume: 174(3-4) Issue: 3-4 Pages: 313-6
  • DOI: 10.1016/j.vetpar.2010.09.004
  • Peer Reviewed
• [Journal Article] Morphological changes and viability of Cryptosporidium parvum sporozoites after excystation in cell-free culture media. (2010)
  • Author(s): Matsubayashi M., et al.
  • Journal Title: Parasitology Volume: 137(13) Pages: 1861-1866
  • Peer Reviewed
• [Journal Article] Survey and molecular characterization of Cryptosporidium and Giardia spp.in owned companion animals, dogs and cats, in Japan (2010)
  • Author(s): Yoshiuchi R., Matsubayashi M., et al.
  • Journal Title: Veterinary Parasitology Volume: 174(3-4) Pages: 313-316
  • Peer Reviewed
• [Journal Article] Survey on gastrointestinal parasites and detection of Cryptosporidium spp
  • Author(s): Ananta SM, Suharno, Hidayat A, Matsubayashi M (CA)
  • Journal Title: on cattle in West Java, Indonesia. Asian Pacific Journal of Tropical Medicine Volume: (In press)
  • Peer Reviewed
• [Presentation] マイクロアレイを用いたEimeria tenella無性生殖期のトランスクリプトーム解析 (2013)
  • Author(s): 松林誠,八田岳士,三好猛晴,アニスザマン,山地佳代子,志村亀夫,坂元君年,笹井和美,磯部尚,北潔,辻尚利
  • Organizer: 第82回日本寄生虫学会大会プログラム・抄録集、83
  • Place of Presentation: 東京医科歯科大学
• [Presentation] マイクロアレイを用いたEimeria tenella 無性生殖期のトランスクリプトーム解析 (2013)
  • Author(s): 松林 誠ら
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京医科歯科大学
• [Presentation] Eimeria tenella 無性生殖 期のトランスクリプトーム解析 (2012)
  • Author(s): 松林 誠, 八田岳士, 三好猛晴, Anisuzzaman, Alim M. Abdul, 山地佳代 子, 志村亀夫, 磯部 尚, 坂元君年, 北 潔, 辻 尚利
  • Organizer: 第10回分子寄生虫・マラリア研究フォーラム. 10,39
  • Place of Presentation: 群馬大学
• [Presentation] Eimeria tenella の鶏盲腸寄生期虫体に対するミトコンド リア呼吸鎖阻害剤の原虫殺滅作用 (2012)
  • Author(s): 松林 誠, 八田岳士, 三好 猛晴, M. ア ニスザマン, 志村 亀夫, 磯部 尚, 坂元 君年, 北 潔, 辻 尚利
  • Organizer: 第154回日本獣医学会講演要旨集, 209
  • Place of Presentation: 岩手大学
• [Presentation] Transcriptome analysis on the asexual second-generation development of Eimeria tenella in the chicken ceca using specific DNA microarray (2012)
  • Author(s): Matsubayashi M, Hatta T, Miyoshi T, Anisuzzaman, Alim MA, Yamaji K, Kameo S, Isobe T, Tsuji N
  • Organizer: Thailand - Japan Joint Conference on Animal Health 2012: The 25th Year Anniversary of National Institute of Animal Health
  • Place of Presentation: Program and Abstracts of Papers, 43-46. 2012.
• [Presentation] Eimeria tenella 侵入型虫体のミト コンドリア呼吸鎖阻害剤 in vitro 感受性 試験 (2012)
  • Author(s): 松林 誠, 八田岳士, 三好猛晴, アニス ザマン, アリム アブドゥル, 山地佳代子, 志村亀夫, 磯部尚, 坂元君年,北 潔, 辻 尚利
  • Organizer: 第81回日本寄生虫学会大会プログ ラム・抄録集, 65
  • Place of Presentation: 兵庫 医科大学
• [Presentation] Transcriptome analysis on the asexual second-generation development of Eimeria tenella in the chicken ceca using specific DNA microarray (2012)
  • Author(s): Makoto Matsubayashi et al.
  • Organizer: Thailand - Japan Joint Conference on Animal Health 2012: The 25th Year Anniversary of National Institute of Animal Health
  • Place of Presentation: Thailand, Bangkok, Rama Garden Hotel
• [Presentation] Eimeria tenella 無性生殖期のトランスクリプトーム解析 (2012)
  • Author(s): 松林 誠ら
  • Organizer: 第10回分子寄生虫・マラリア研究フォーラム
  • Place of Presentation: 群馬大学
• [Presentation] Eimeria tenella侵入型虫体のミトコンドリア呼吸鎖阻害剤in vitro感受性試験 (2012)
  • Author(s): 松林誠, ら
  • Organizer: 第81回日本寄生虫学会大会
  • Place of Presentation: 兵庫医科大学
• [Presentation] 鶏Eimeria外界発育ステージの網羅的発現遺伝子解析 (2011)
  • Author(s): 松林誠,八田岳士,三好猛晴,Mアニスザマン,アリムアブドゥル,山地佳代子,志村亀夫,磯部尚,辻尚利
  • Organizer: 第152回日本獣医学会講演要旨集,200
  • Place of Presentation: 大阪府立大学
• [Presentation] Identification ofexpressed genes in thesecond-generation stage of Eimeria tenella by microarray analysis (2011)
  • Author(s): Matsubayashi M, Hatta T, Miyoshi T, Anisuzzaman, Alim MA, Yamaji K, Shimura K, Isobe T, Tsuji N
  • Organizer: 17th Japanese-German Cooperative Symposium on Protozoan Disease. Program and Abstracts of Papers, 26
  • Place of Presentation: 奈良女子大学
• [Presentation] Eimeria tenellaの胞子形成期およびスポロゾイトのトランスクリプトーム解析 (2011)
  • Author(s): 松林誠,八田岳士,三好猛晴,M.アニスザマン,アリムアブドゥル,山地佳代子,志村亀夫,磯部尚,辻尚利
  • Organizer: 第80回日本寄生虫学会大会プログラム・抄録集,P56
  • Place of Presentation: 東京慈恵会医科大学
• [Presentation] Identification of expressed genes in the second-generation stage of Eimeriatenella by microarray analysis (2011)
  • Author(s): Makoto Matsubayashi, et al
  • Organizer: 17th Japanese-German Cooperative Symposium on Protozoan Disease
  • Place of Presentation: 奈良女子大学
• [Presentation] 鶏Eimeria外界発育ステージの網羅的発現遺伝子解析 (2011)
  • Author(s): 松林誠, ら
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 大阪府立大学
• [Book] 細菌・真菌・ウィルス便覧』書籍(技術情報協会) (2013)
  • Author(s): 松林誠
  • Publisher: Eimeria spp
• [Book] 「Eimeria」 細菌・真菌・ウィルス便覧 (2013)
  • Author(s): 松林 誠 (分担)
  • Publisher: 技術情報協会
• [Book] 本邦で検出された Cryptosporidium andersoni novel type について(Recent Advances in Medical Science: Parasites and their Human and Animal Hosts ((医 科学の進歩:寄生虫とその宿主たるヒトと 動物)). 編集、宇仁茂彦、木俣勲 (2010)
  • Author(s): 松林 誠、古屋敷(長野)早希、木俣 勲、木太俊雅、古家 優、谷 浩行、笹井和美
  • Publisher: 版権: 第65回日本寄生虫学会西日本支部大会事 務局.