Targeting adenovirus for glioma stem cell with adenovirus library

• Project/Area Number: 22700919
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Clinical oncology
• Research Institution: Hiroshima University
• Principal Investigator: NISHIMOTO Takeshi 広島大学, 医歯薬学総合研究科, 研究員 (40450580)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 遺伝子治療 / グリオーマ / 腫瘍幹細胞 / アデノウィルス / ライブラリー / アデノウイルス

Research Abstract

We have developed a direct selection method of targeted vector from peptide library displayed on an adenovial fiber knob(more than 2x105 level) to overcome the limitation that many cell type-specific ligands for targeted adenovirus vectors are unknown. First, glioma stem cells were prepared from glioma cell line(D54), a peptide-display adenovirus library was screened on them and particular peptide sequences were selected.