Controlling the motion of DNA motion near nanopore for DNA sensing applications

• Project/Area Number: 22710117
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Aoyama Gakuin University
• Principal Investigator: MITSUI Toshiyuki 青山学院大学, 理工学部, 准教授 (40406814)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ナノバイオ / 生物物理 / マイクロ・ナノデバイス

Research Abstract

By observing the motion of DNA’ s near nanopore before their translocation throughnanopore, we have estimated the electric field near the nanopore. The rate of the DNA translocations through nanopore depends on the gate voltage on the nanopore membrane relative to the cis voltage. We also estimated the clogging probability of DNA’ s into nanopore by various bias voltages between trans and cis chambers.

Research Products

• [Journal Article] Directly Observing the Motion of DNA Molecules near Solid-State Nanopores (2012)
  • Author(s): Ando G, Hyun C, Li J, Mitsui T
  • Journal Title: ACS Nano Volume: 6(11) Pages: 10090-7
• [Journal Article] Adsorbed water-molecule hexagons with unexpected rotations in islands on Ru(0001) and Pd(111) (2012)
  • Author(s): Sabine Maier, Ingeborg Stass, Toshiyuki Mitsui, Peter J. Feibelman, Konrad Thurmer, and Miquel Salmeron
  • Journal Title: Phys. Rev. B Volume: 85 Pages: 155434-9
• [Journal Article] Directly Observing the Motion of DNA Molecules near Solid-State Nanopores (2012)
  • Author(s): Toshiyuki Mitsui (Genki Ando)
  • Journal Title: ACS Nano Volume: 6 Pages: 10090-7
  • Peer Reviewed
• [Journal Article] Adsorbed water-molecule hexagons with unexpected rotations in islands on Ru(0001) and Pd(111) (2012)
  • Author(s): Sabine Maier, Ingeborg Stass, Toshiyuki Mitsui, Peter J.Feibelman, Konrad Thurmer, Miquel Salmeron
  • Journal Title: Physical Review B Volume: (To be published)(掲載決定)
  • Peer Reviewed
• [Presentation] FET ナノポア近傍の DNA の挙動解析 (2013)
  • Author(s): 安藤元気,杉本 学,山田一輝,三井敏之
  • Organizer: 春季 第60回 応用物理学会学術講演会
  • Place of Presentation: 神奈川工科大学
  • Year and Date: 2013-03-29
• [Presentation] Cell migration under ultrasound irradiations in micrometer scale (2013)
  • Author(s): Shinya Murakami , Yo Otsuka , Yusuke Oshima , Atsuhiko Hikita , Toshiyuki Mitsui
  • Place of Presentation: Baltimore
  • Year and Date: 2013-03-21
• [Presentation] Cell migration under ultrasound irradiations in micrometer scale (2013)
  • Author(s): Shinya Murakami（Toshiyuki Mitsui）
  • Organizer: アメリカ物理学会
  • Place of Presentation: ボルチモア、メリーランド州、アメリカ
• [Presentation] ラマン分光法による細胞分化の識別 (2012)
  • Author(s): 高根沢聡太,盛田伸一,廣島通夫,三井敏之,尾崎幸洋,佐甲靖志
  • Organizer: 春季 第59回 応用物理学会学術講演会
  • Place of Presentation: 早稲田大学
  • Year and Date: 2012-03-16
• [Presentation] 表面増強ラマン散乱を用いた局所的な HeLa 細胞の分析 (2012)
  • Author(s): 杉本 学,大塚 陽,村上真也,三井敏之
  • Organizer: 春季 第59回 応用物理学会学術講演会
  • Place of Presentation: 早稲田大学
  • Year and Date: 2012-03-16
• [Presentation] FET ナノポアによる DNA の通過解析とその制御青学大理工 (2012)
  • Author(s): 守屋裕樹,安藤元気,塚平健太,三井敏之
  • Organizer: 春季 第59回 応用物理学会学術講演会
  • Place of Presentation: 早稲田大学
  • Year and Date: 2012-03-16
• [Presentation] 表面増強ラマン散乱を用いた局所的なHeLa細胞の分析 (2012)
  • Author(s): 杉本学, 大塚陽, 村上真也, 三井敏之
  • Organizer: 春季第59回応用物理学会学術講演会
  • Place of Presentation: 早稲田大学、東京都
  • Year and Date: 2012-03-16
• [Presentation] ラマン分光法による細胞分化の識別 (2012)
  • Author(s): 高根沢聡太, 盛田伸一, 廣島通夫, 三井敏之, 尾崎幸洋, 佐甲靖志
  • Organizer: 春季第59回応用物理学会学術講演会
  • Place of Presentation: 早稲田大学、東京都
  • Year and Date: 2012-03-16
• [Presentation] FETナノポアによるDNAの通過解析とその制御 (2012)
  • Author(s): 守屋裕樹, 安藤元気, 塚平健太, 三井敏之
  • Organizer: 春季第59回応用物理学会学術講演会
  • Place of Presentation: 早稲田大学、東京都
  • Year and Date: 2012-03-16
• [Presentation] Direct observation of DNA translocation influenced by electrically gated nanopores (2012)
  • Author(s): Genki Ando , Hiroki Moriya , Kenta Tsukahira , Satoshi Yano , Toshiyuki Mitsui
  • Organizer: American Physical Society, March Meeting 2012
  • Place of Presentation: アメリカ、ボストン
  • Year and Date: 2012-02-27
• [Presentation] Direct observation of DNA translocation influenced by electrically gated nanopores (2012)
  • Author(s): Genki Ando, Hiroki Moriya, Kenta Tsukahira, Satoshi Yano, Toshiyuki Mitsui
  • Organizer: American Physical Society, March Meeting 2012
  • Place of Presentation: Boston Convention Center Boston, Massachusetts, USA
  • Year and Date: 2012-02-27
• [Presentation] Controlled cell migration with ultrasound (2012)
  • Author(s): Shinya Murakami, Yo Otsuka, Manabu Sugimoto, Toshiyuki Mitsui
  • Organizer: The 50th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Nagoya, Japan
• [Presentation] Evaluation of Ultrasound Irradiation effects on Cells by using Raman Spectroscopy (2012)
  • Author(s): Manabu Sugimoto, Yo Otsuka, Shinya Murakami, Toshiyuki Mitsui
  • Organizer: The 50th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Nagoya, Japan
• [Presentation] Interaction of DNA molecules with nanopore studied byfluorescence microscopy (2012)
  • Author(s): Genki Ando, Kazuteru Yamada, Toshiyuki Mitsui
  • Organizer: The 50th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Nagoya, Japan
• [Presentation] Interaction of DNA molecules with nanopore studied by fluorescence microscopy (2012)
  • Author(s): Genki Ando（Toshiyuki Mitsui）
  • Organizer: The 50th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: 名古屋大学
• [Presentation] Evaluation of Ultrasound Irradiation effects on Cells by using Raman Spectroscopy (2012)
  • Author(s): Manabu Sugimoto（Toshiyuki Mitsui）
  • Organizer: The 50th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: 名古屋大学
• [Presentation] Controlled cell migration with ultrasound (2012)
  • Author(s): Shinya Murakami（Toshiyuki Mitsui）
  • Organizer: The 50th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: 名古屋大学
• [Presentation] A phase diagram of cell differentiation based on Raman spectroscopic measurements (2011)
  • Author(s): Shin-ichi Morita, Sota Takanezawa, Michio Hirohima, Toshiyuki Mitsui, Yasuhi Sako
  • Organizer: 第49回日本生物物理学会
  • Place of Presentation: 兵庫県立大学・姫路書写キャンパス
  • Year and Date: 2011-09-16
• [Presentation] Fluorescence microscope study of DNA motion near silicon based nano scale pore (2011)
  • Author(s): Genki Ando, Kenta Tsukahira, Yuki Moriya, Toshiyuki Mitsui
  • Organizer: 第49回日本生物物理学会
  • Place of Presentation: 兵庫県立大学・姫路書写キャンパス
  • Year and Date: 2011-09-16
• [Presentation] Analysis of bactericidal activity on Escherichia coli by Ag nanoparticle in medium and by its surface coating in sub millimeter chamber (2011)
  • Author(s): Yo Otsuka, Jyunki Kawagoe, ShinyaMurakami, Satoshi Yano, ToshiyukiMitsui
  • Organizer: 第49回日本生物物理学会
  • Place of Presentation: 兵庫県立大学・姫路書写キャンパス
  • Year and Date: 2011-09-16
• [Presentation] Fluorescence microscope study of DNA motion near silicon based nanoscalepore (2011)
  • Author(s): Genki Ando, Kenta Tsukahira, Yuki Moriya, Toshiyuki Mitsui
  • Organizer: 第49回日本生物物理学会
  • Place of Presentation: 兵庫県立大学・姫路書写キャンパス、姫路、兵庫県
  • Year and Date: 2011-09-16
• [Presentation] A phase diagram of cell differentiation based on Raman spectroscopic measurements (2011)
  • Author(s): Shin-ichi Morita, Sota Takanezawa, Michio Hirohima, Toshiyuki Mitsui, Yasuhi Sako
  • Organizer: 第49回日本生物物理学会
  • Place of Presentation: 兵庫県立大学・姫路書写キャンパス、姫路、兵庫県
  • Year and Date: 2011-09-16
• [Presentation] Analysis of bactericidal activity on Escherichia coli by Ag nanoparticle in medium and by its surface coating in sub millimeter chamber (2011)
  • Author(s): Yo Otsuka, Jyunki Kawagoe, Shinya Murakami, Satoshi Yano, Toshiyuki Mitsui
  • Organizer: 第49回日本生物物理学会
  • Place of Presentation: 兵庫県立大学・姫路書写キャンパス、姫路、兵庫県
  • Year and Date: 2011-09-16
• [Presentation] Direct observation of DNA motions into solid state nanopore under applied electrical potentials on conductive surface (2011)
  • Author(s): Yoshitaka Hayashi, Genki Ando, Ichiro Idutsu, Toshiyuki Mitsui
  • Organizer: American Physical Society, March Meeting 2011
  • Place of Presentation: アメリカ、ダラス
  • Year and Date: 2011-03-23
• [Presentation] Direct observation of DNA motions into solid state nanopore under applied electrical potentials on conductive surface (2011)
  • Author(s): Yoshitaka Hayashi, Genki Ando, Ichiro Idutsu, Toshiyuki Mitsui
  • Organizer: American Physical Society, APS March Meeting 2011,
  • Place of Presentation: アメリカ、ダラス
  • Year and Date: 2011-03-23
• [Presentation] TEM ビームによるナノポア形成機構の解析 (2011)
  • Author(s): 石田 猛,柳 至,大浦 剛,板橋直志,山本治朗,赤堀玲奈,小沢 理,穴沢 隆,高口雅成,三井敏之
  • Organizer: 春季 第58回 応用物理学会学術講演会
  • Place of Presentation: 神奈川県
  • Year and Date: 2011-03-15
• [Presentation] ナノポアシーケンシングに向けたナノポアデバイスのイオン電流 (2011)
  • Author(s): 赤堀玲奈,大浦 剛,小沢 理,穴沢 隆,石田 猛,柳 至,板橋直志,山本治朗,高口雅成,三井敏之
  • Organizer: 春季 第58回 応用物理学会学術講演会
  • Place of Presentation: 神奈川県
  • Year and Date: 2011-03-15
• [Presentation] ラマン分光による生細胞の分化ダイナミクスの研究 (2011)
  • Author(s): 盛田伸一, 高根沢聡太, 廣島通夫, 村上拓郎, 川島徳道, 三井敏之, 佐甲靖志
  • Organizer: 春季 第58回 応用物理学会学術講演会
  • Place of Presentation: 神奈川県
  • Year and Date: 2011-03-15
• [Presentation] TEMビームによるナノポア形成機構の解析 (2011)
  • Author(s): 石田猛, 柳至, 大浦剛, 板橋直志, 山本治朗, 赤堀玲奈, 小沢理, 穴沢隆, 高口雅成, 三井敏之
  • Organizer: 春季 第58回 応用物理学会学術講演会
  • Place of Presentation: 神奈川県神奈川工科大学(誌上発表)
  • Year and Date: 2011-03-15
• [Presentation] Raman analysis of the cell differentiation using two-dimensional correlation spectroscopy (2011)
  • Author(s): 高根沢聡太、盛田伸一、廣島通夫、三井敏之、尾崎幸洋、佐甲靖志
  • Organizer: 秋季 応用物理学会学術講演会
  • Place of Presentation: 山形大学
• [Presentation] 細胞分化のラマン分光分析 (2011)
  • Author(s): 高根沢聡太、盛田伸一、廣島通夫、三井敏之、尾崎幸洋、佐甲靖志
  • Organizer: 日本分析化学会第60 年会
  • Place of Presentation: 名古屋大学東山キャンパス
• [Presentation] Raman Spectroscopy and Correlation Analysis on Live Cell Dynamics (2011)
  • Author(s): Sota Takanezawa, Shin-ichi Morita,Michio Hiroshima, Takurou N. Murakami, Norimichi Kawashima,Toshiyuki Mitsui, Yasushi Sako
  • Organizer: Sixth International Meeting on 2 - Dimensional Correlation Spectroscopy
  • Place of Presentation: Sonoma County, California
• [Presentation] Raman Spectroscopy and Correlation Analysis on Live Cell Dynamics (2011)
  • Author(s): Sota Takanezawa, Shin-ichi Morita, Michio Hiroshima, Takurou N.Murakami, Norimichi Kawashima, Toshiyuki Mitsui, Yasushi Sako
  • Organizer: Sixth International Meeting on 2-Dimensional Correlation Spectroscopy
  • Place of Presentation: Sonoma, Doubletree Hotel, California, USA
• [Presentation] Raman analysis of the cell differentiation using two-dimensional correlation spectroscopy (2011)
  • Author(s): 高根沢聡太、盛田伸一、廣島通夫、三井敏之、尾崎幸洋、佐甲靖志
  • Organizer: 秋季応用物理学会学術講演会
  • Place of Presentation: 山形大学、小白川キャンパス、山形県
• [Presentation] DNA dynamics near modified solid state nanopore (2010)
  • Author(s): Hayashi, Yoshitaka; Ando, Genki; Kobayashi, Kaya; Mitsui, Toshiyuki
  • Organizer: American Physical Society, March Meeting 2010
  • Place of Presentation: アメリカ、オレゴン
  • Year and Date: 2010-03-17
• [Presentation] Raman spectroscopy for non-invasive microscopic analysis of biological systems and single live cells (2010)
  • Author(s): 盛田 伸一 高根沢 聡太 廣島 通夫 三井敏之 佐甲 靖志
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学川内キャンパス
• [Book] マクロ観察と新型顕微鏡技法Q&A (2010)
  • Author(s): 朝倉健太郎, 他
  • Total Pages: 4
  • Publisher: アグネ承風社
• [Remarks]
  • URL: http://www.phys.aoyama.ac.jp/~w3-mitsui/index.html
• [Remarks] 青山学院大学 研究者情報
  • URL: http://raweb.jm.aoyama.ac.jp/aguhp/KgApp?kyoinId=ymdggkyeggy