Characterization of sugar-sugar glycosyltransferases involved in plant secondary metabolism.

• Project/Area Number: 22710218
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: Nagoya City University
• Principal Investigator: TERASAKA Kazuyoshi 名古屋市立大学, 大学院・薬学研究科, 助教 (60405214)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 植物二次代謝 / 低分子有機化合物配糖体 / 糖転移酵素

Research Abstract

We isolated cDNA clones encoding novel sugar-sugar glucosyltransferases catalyzing 1, 6-glucosylation from Gardenia jasminoides and Glehnia littoralis that produce gentiobioside compounds in planta. These recombinant proteins exhibited a unique glucosyl chain elongation activity forming gentiobioside.

Research Products

• [Journal Article] UGT75L6 and UGT94E5 mediate sequential glucosylation of crocetin to crocin in Gardenia jasminoides (2012)
  • Author(s): Mai Nagatoshi
  • Journal Title: FEBS Letters Volume: 586 Issue: 7 Pages: 1055-1061
  • DOI: 10.1016/j.febslet.2012.03.003
  • Peer Reviewed
• [Journal Article] Iridoid-specific Glucosyltransferase from Gardenia jasminoides (2011)
  • Author(s): Mai Nagatoshi, Kazuyoshi Terasaka, Akito Nagatsu, Hajime Mizukami
  • Journal Title: The Journal of Biological Chemistry Volume: 286巻 Issue: 37 Pages: 32866-32874
  • DOI: 10.1074/jbc.m111.242586
  • Peer Reviewed
• [Presentation] 組換え酵素カクテルを用いたzeaxanthinからcrocinの生産 (2012)
  • Author(s): 永利麻衣
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2012-03-29
• [Presentation] ハマボウフウ培養細胞由来クマリン配糖化酵素の機能解析 (2011)
  • Author(s): 岡本隼己
  • Organizer: 第29回日本植物細胞分子生物学会(福岡)大会
  • Place of Presentation: 九州大学(福岡市)
  • Year and Date: 2011-09-08
• [Presentation] ハマボウフウ培養細胞由来coumarin配糖化酵素のクローニングと機能解析 (2011)
  • Author(s): 岡本隼己
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: ツインメッセ静岡(静岡市)
  • Year and Date: 2011-03-31
• [Presentation] ハマボウフウ培養細胞由来coumarin配糖化酵素のクローニングと機能解析 (2011)
  • Author(s): 岡本隼己
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: ツインメッセ静岡
  • Year and Date: 2011-03-31
• [Presentation] クチナシ培養細胞由来crocetin配糖化酵素の単離と機能解析 (2010)
  • Author(s): 寺坂和祥
  • Organizer: 第28回日本植物細胞分子生物学会(仙台)大会
  • Place of Presentation: 東北大学雨宮キャンパス(仙台市)
  • Year and Date: 2010-09-03
• [Presentation] クチナシ培養細胞由来crocetin配糖化酵素の単離と機能解析 (2010)
  • Author(s): 寺坂和祥
  • Organizer: 第28回日本植物細胞分子生物学会(仙台)大会
  • Place of Presentation: 東北大学雨宮キャンパス
  • Year and Date: 2010-09-03