Development of artificial aminoacylated tRNA with high efficiency of turnover
| Project/Area Number |
22750146
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Chemistry related to living body
|
|---|
| Research Institution | Tokyo Institute of Technology |
|---|
Principal Investigator |
OHKUBO Akihiro 東京工業大学, 大学院・生命理工学研究科, 助教 (60376960)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Keywords | RNA合成 / アミノアシルtRNA / ホスホロアミダイト法 / アミノ酸 / 固相合成 |
|---|
| Research Abstract |
In this study we could synthesize base-labile aminoacylated RNA oligonucleotides using N-unprotected 5'-phosphoramidite RNA units on the nucleoside-loaded polymer supports via a silyl linker which can be cleaved under neutral conditions. These results have prompted us to study the property of the RNA complexes between the aminoasylated RNAs and corresponding 5'-long RNAs. These additional studies are now underway.
|
Report
(3 results)
Research Products
(31 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Insights into the DNA stabilizing contributions of a bicyclic cytosine analogue : crystal structures of DNA duplexes containing 7, 8-dihydropyrido[ 2, 3-d] pyrimidin-2-one. E. C. M. Juan2010
Author(s)
S. Shimizu, X. Ma, T. Kurose, T. Haraguchi, F. Zhang, M. Tsunoda, A. Ohkubo, M. Sekine, T. Shibata, C. L. Millington, D. M. Williams, A. Takenaka
-
Journal Title
Nucleic Acids Res
Volume: 38
Pages: 6737-6745
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
