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Water-in-oil droplet formation by electrostatic atomization and its application to biotechnology

Research Project

Project/Area Number 22760207
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Power engineering/Power conversion/Electric machinery
Research InstitutionToyohashi University of Technology

Principal Investigator

KURITA Hirofumi  豊橋技術科学大学, 大学院・工学研究科, 助教 (70512177)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords静電噴霧 / 油中液滴 / バイオ応用 / マイクロリアクター / 静電気生物応用 / 液滴生成 / 遺伝子導入
Research Abstract

Water-in-oil(W/O) droplet formation by electrostatic atomization and manipulation of the individual droplet by electrostatic force were studied. A method to generate W/O droplets using electrostatic field was investigated. The method can control the size of water droplets by adjusting the applied DC high voltage and these water droplets are stable without a surfactant due to electrical charge. In addition, a novel method to transport and fuse W/O droplets using non-uniform electrostatic field was also investigated. Furthermore, gene transformation by using DC electric field was carried out. This method can reduce sample volume smaller than that of conventional method. In conclusion, these methods are expected to be a new tool in miniaturized bioengineering.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (21 results)

All 2011 2010 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (12 results) Remarks (3 results)

  • [Journal Article] Single-molecule measurement of strand breaks on large DNA induced by atmospheric pressure plasma jet2011

    • Author(s)
      H.Kurita, T.Nakajima, H.Yasuda, K.Takashima, A.Mizuno, J.I.B.Wilson, S.Cunningham
    • Journal Title

      Applied Physics Letters

      Volume: 99 Issue: 19

    • DOI

      10.1063/1.3660581

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Direct observation method of individual single-stranded DNA molecules using fluorescent replication protein A2011

    • Author(s)
      Oshige M, Kawasaki S, Takano H, Yamaguchi K, Kurita H, Mizuno T, Matsuura S, Mizuno A, Katsura S.
    • Journal Title

      Journal Fluorescence

      Volume: 21(3) Issue: 3 Pages: 1189-1194

    • DOI

      10.1007/s10895-010-0797-8

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 静電微粒子化を用いたエマルション生成2010

    • Author(s)
      青木博則, 栗田弘史, Thierry Paillat, 高島和則, 水野彰
    • Journal Title

      静電気学会誌

      Volume: vol.34 Pages: 25-30

    • NAID

      10026875012

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A kinetic analysis of strand breaks on large DNA induced by cigarette smoke extract2010

    • Author(s)
      H.Kurita, T.Takata, H.Yasuda, K.Takashima, A.Mizuno
    • Journal Title

      Chemical Physics Letters

      Volume: 493 Pages: 165-169

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biological Evaluation of DNA Damage in Bacteriophages Inactivated by Atmospheric Pressure Cold Plasma2010

    • Author(s)
      H.Yasuda, T.Miura, H.Kurita, K.Takashima, A.Mizuno
    • Journal Title

      Plasma Processes and Polymers

      Volume: 7 Pages: 301-308

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new DNA combing method for biochemical analysis2010

    • Author(s)
      M.Oshige, K.Yamaguchi, S.Matsuura, H.Kurita, A.Mizuno, S.Katsura
    • Journal Title

      Analytical Biochemistry

      Volume: 400 Pages: 145-147

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 直流電界と油中液滴を用いた遺伝子導入法の開発2011

    • Author(s)
      浅田淳,青木博則,栗田弘史,安田八郎,高島和則,水野彰
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜,横浜市
    • Year and Date
      2011-12-13
    • Related Report
      2011 Final Research Report
  • [Presentation] 直流電界と油中液滴を用いた遺伝子導入法の開発2011

    • Author(s)
      浅田淳, 青木博則, 栗田弘史, 安田八郎, 高島和則, 水野彰
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Water-in-oil droplet generation and manipulation using an electrostatic field for miniaturized reaction systems2011

    • Author(s)
      Atsushi Asada, Hironori Aoki, Hirofumi Kurita, Hachiro Yasuda, Kazunori Takashima, and Akira Mizuno
    • Organizer
      2nd ISNPEDADM-2011 New electrical technologies for environment
    • Place of Presentation
      Noumea, New Caledonia, France
    • Year and Date
      2011-11-17
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] A Novel Gene Transformation Technique using Water-in-oil Droplet in an Electrostatic Field2011

    • Author(s)
      Atsushi Asada, Hironori Aoki, Hirofumi Kurita, Hachiro Yasuda, Kazunori Takashima, and Akira Mizuno
    • Organizer
      IEEE Industry Applications Society Annual Meeting
    • Place of Presentation
      Orland, USA
    • Year and Date
      2011-10-10
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 電界中の油中液滴を用いる遺伝子導入法の開発2011

    • Author(s)
      浅田淳,青木博則,栗田弘史,安田八郎,高島和則,水野彰
    • Organizer
      第35回静電気学会全国大会
    • Place of Presentation
      東京理科大学,東京都
    • Year and Date
      2011-09-13
    • Related Report
      2011 Final Research Report
  • [Presentation] 電界中の油中液滴を用いる遺伝子導入法の開発2011

    • Author(s)
      浅田淳, 青木博則, 栗田弘史, 安田八郎, 高島和則, 水野彰
    • Organizer
      第35回静電気学会全国大会
    • Place of Presentation
      東京理科大学
    • Year and Date
      2011-09-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Fluorescence Observation and Manipulation of Individual DNA Molecules in a Microfluidic Channel2010

    • Author(s)
      Hirofumi Kurita, Hironori Aoki, Tatsuya Takata, Atsushi Asada, Hachiro Yasuda, Kazunori Takashima, Akira Mizuno
    • Organizer
      2010 International Symposium on Micro-Nano Mechatronics and Human Science
    • Place of Presentation
      名古屋大学
    • Year and Date
      2010-11-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Generation of Water Droplet in Fluorocarbon using Electrostatic Atomization2010

    • Author(s)
      Hironori Aoki, Hirofumi Kurita, Thierry Paillat, Kazunori Takashima, and Akira Mizuno
    • Organizer
      IEEE Industry Applications Society Annual Meeting
    • Place of Presentation
      Houston, USA
    • Year and Date
      2010-10-05
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 不平等電界を用いた油中液滴輸送・融合技術の開発2010

    • Author(s)
      青木博則,栗田弘史,安田八郎,高島和則,水野彰
    • Organizer
      第34回静電気学会全国大会
    • Place of Presentation
      鳥取大学,鳥取県
    • Year and Date
      2010-09-15
    • Related Report
      2011 Final Research Report
  • [Presentation] 不平等電界を用いた油中液滴輸送・融合技術の開発2010

    • Author(s)
      青木博則, 栗田弘史, 安田八郎, 高島和則, 水野彰
    • Organizer
      第34回静電気学会全国大会
    • Place of Presentation
      鳥取大学
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] Recent Development of Non-thermal Plasma Technologies in Environmental Remediation and Bacterial Inactivation2010

    • Author(s)
      Hirofumi Kurita, Hachiro Yasuda, Kazunori Takashima, Akira Mizuno
    • Organizer
      The 11th International Workshop on Advanced Plasma Processing and Diagnostics (The 11th Korea-Japan Workshop in Jeju, Korea)
    • Place of Presentation
      Ramada Plaza Hotel Jeju(Korea)
    • Year and Date
      2010-07-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] タバコ煙抽出液の直鎖状長鎖DNAに対する切断活性の1分子直接観察に基づく速度論解析2010

    • Author(s)
      高田達也, 栗田弘史, 安田八郎, 高島和則, 水野彰
    • Organizer
      第37回日本トキシコロジー学会学術年会
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2010-06-16
    • Related Report
      2010 Annual Research Report
  • [Remarks] ホームページ

    • URL

      http://ens.tut.ac.jp/electrostatics/

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://ens.tut.ac.jp/electrostatics/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://ens.tut.ac.jp/electrostatics/

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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