High-throughput analysis and engineering of fungi transcription factor binding sequences.
| Project/Area Number |
22760605
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Nagoya University |
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Principal Investigator |
KOJIMA Takaaki 名古屋大学, 生命農学研究科, 助教 (40509080)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 転写因子 / プロモーター活性 / エマルジョンPCR / 糸状菌 / ハイスループットスクリーニング / Aspergillus nidulans / DNA-タンパク質相互作用 / FACS / プロモーター / バイオプロセス / 発現制御 / 生体機能利用 / 核酸 / 蛋白質 / 遺伝子 |
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| Research Abstract |
Bead display is a novel library construction method in which clonal DNA moleculesare displayed on a large population of microbeads. Comprehensive analysis of DNA binding regions of a fungal transcription factor, AmyR, was carried out with a random DNA library or A. nidulans genome library by using bead display. As the results, the extraction of AmyR binding motifs and the selection of various binding sites of AmyR were observed. In addition, a high-throughput screening method for promoter activity was developed by using bead display and a ligase ribozyme
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] Comprehensive Analysis of the DNA-Binding Specificity of an <i>Aspergillus nidulans</i> Transcription Factor, AmyR, Using a Bead Display System2012
Author(s)
Wang, P., Kojima, T., Kobayashi, T., Nakano, H.
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 76
Issue: 6
Pages: 1128-1134
DOI
NAID
ISSN
0916-8451, 1347-6947
Related Report
Peer Reviewed
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[Presentation] High-throughput Screening of DNA Binding Sites for Fungus Transcription Factor, AmyR, Using Beads Display System of DNA2010
Author(s)
Kojima, T., Hashimoto, Y., Kato, M., Kobayashi, T. and Nakano, H
Organizer
グローバルCOE第4回リトリート
Place of Presentation
豊橋
Related Report
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