Regulation of DNA methylation at SINEs during mouse male germ-cell development

• Project/Area Number: 22770009
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Genetics/Genome dynamics
• Research Institution: Kyushu University
• Principal Investigator: ICHIYANAGI Kenji 九州大学, 生体防御医学研究所, 助教 (70401560)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ゲノム機能・発現 / エピジェネティック制御 / レトロトランスポゾン / エピジェネティクス / 生殖細胞 / DNAメチル化 / クロマチンバウンダリー

Research Abstract

To elucidate regulation of SINE retrotransposons during mammalian germ-cell development, DNA methylation states of mouse B1 and B2 SINE were studied. This study revealed(1) SINEs become demethylated in primordial germ cells, then re-methylated in fatal prospermatogonia,(2) after birth, they keep methylated during spermatogenesis,(3) SINE copies neighboring transcription start sites are involved in regulation of the gene expression,(4) the germ cell-specific small RNAs(piRNAs) are dispensable for SINE methylation and regulation, and(5) SINE methylation depends on Dnmt3a with varied dependencies according to their genomic and nuclear locations. Thus, the regulation of SINE methylation differs significantly from those of LINEs and endogenous retroviruses.

Research Products

• [Journal Article] Inhibition of MspI cleavage activity by hydroxymethylation of the CpG site (2012)
  • Author(s): Ichiyanagi, K
  • Journal Title: A concern for DNA modification studies using restriction endonucleases. Volume: 7 Pages: 131-136
• [Journal Article] Inhibition of MspI cleavage activity by hydroxymethylation of the CpG site : A concern for DNA modification studies using restriction endonucleases (2012)
  • Author(s): Ichiyanagi, K.
  • Journal Title: Epigenetics Volume: 7 Issue: 2 Pages: 131-136
  • DOI: 10.4161/epi.7.2.18909
  • Peer Reviewed
• [Journal Article] Locus-and domain-dependent control of DNA methylation at mouse B1 retrotransposons during male germ cell development (2011)
  • Author(s): Ichiyanagi, K., Li, Y., Watanabe, T., Ichiyanagi, T., Fukuda, K., Kitayama, J., Yamamoto, Y., Kuramochi-Miyagawa, S., Nakano, T., Yabuta, Y., Seki, Y., Saitou, M., and Sasaki, H
  • Journal Title: Genome Res Volume: 21 Pages: 2058-2066
• [Journal Article] Role for piRNAs and non-coding RNA in de novo DNA methylation of (2011)
  • Author(s): Watanabe, T., Tomizawa, S., Mitsuya, K., Totoki, Y., Yamamoto, Y., Kuramochi-Miyagawa, S., Iida, N., Hoki, Y., Murphy, P. J., Toyoda, A., Gotoh, K., Hiura, H., Arima, T., Fujiyama, A., Sado, T., Shibata, T., Nakano, T., Lin, H., Ichiyanagi, K., Soloway, P.D. and Sasaki, H.
  • Journal Title: the imprinted mouse Rasgrf1 locusthe imprinted mouse Rasgrf1 locusthe imprinted mouse Rasgrf1 locus Volume: 332 Pages: 848-852
• [Journal Article] Locus- and domain-dependent control of DNA methylation at mouse B1 retrotransposons during male germ cell development (2011)
  • Author(s): Ichiyanagi, T., Fukuda, K., Kitayama, J., Yamamoto, Y., Kuramochi-Miyagawa, S., Nakano, T., Yabuta, Y., Seki, Y., Saitou, M., & Sasaki, H
  • Journal Title: Genome Res Volume: 21 Issue: 12 Pages: 2058-2066
  • DOI: 10.1101/gr.123679.111
  • Peer Reviewed
• [Journal Article] Role for piRNAs and non-coding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus (2011)
  • Author(s): Watanabe, T., Tomizawa, S., Mitsuya, K., Totoki, Y., Yamamoto, Y., Kuramochi-Miyagawa, S., Iida, N., Hoki, Y., Murphy, P.J., Toyoda, A., Gotoh, K., Hiura, H., Arima, T., Fujiyama, A., Sado, T., Shibata, T., Nakano, T., Lin, H., Ichiyanagi, K., Soloway, P.D., Sasaki, H
  • Journal Title: Science Volume: 332 Issue: 6031 Pages: 848-52
  • DOI: 10.1126/science.1203919
  • Peer Reviewed
• [Journal Article] Role for piRNAs and non-coding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus. (2011)
  • Author(s): Watanabe, T., Tomizawa, S., Mitsuya, K., Totoki, Y., Yamamoto, Y., Kuramochi-Miyagawa, S., Iida, N., Hoki, Y., Murphy, P.J., Toyoda, A., Gotoh, K., Hiura, H., Arima, T., Fujiyama, A., Sado, T., Shibata, T., Nakano, T., Lin, H., Ichiyanagi, K., Soloway, P.D., Sasaki
  • Journal Title: Science Volume: (印刷中)
  • Peer Reviewed
• [Presentation] Locus- and domain-dependent control of DNA methylation at mouse SINEs during male germ cell development (2012)
  • Author(s): Kenji Ichiyanagi, Yungfeng Li, Tomoko Ichiyanagi, Keigo Hirafuku, Hiroyuki Sasaki
  • Organizer: 3^<rd> international symposium "Genomic Impact of eukaryotic transposable elements"
  • Place of Presentation: 米国カリフォルニア州
  • Year and Date: 2012-02-25
• [Presentation] Locus-and domain-dependent control of DNA methylation at mouse SINEs during male germ cell development (2012)
  • Author(s): Ichiyanagi, K., Li, Y., Ichiyanagi, T., Hirafuku, K., and Sasaki H.
  • Organizer: 3rd international conference"Genomic impact of eukaryotic transposable elements
  • Place of Presentation: CA, U. S. A.
• [Presentation] Locusand domain-dependent control of DNA methylation at mouse B1 retrotransposon during male germ cell development (2012)
  • Author(s): 2) Ichiyanagi, K., Li, Y., Watanabe, T., Fukuda, K., Kitayama, J., Seki, Y., Yabuta, Y., Yamamoto, Y., Ichiyanagi, T., and Sasaki H.
  • Organizer: Gordon Research Conference
  • Place of Presentation: MA, U. S. A
• [Presentation] マウスB2 SINEのDNAメチル化制御機構 (2011)
  • Author(s): 平福啓一伍、一柳朋子、三宅美保、佐々木裕之、一柳健司
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] SINE配列から見たマウス生殖細胞のエピゲノム制御機構 (2011)
  • Author(s): 一柳健司
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 2011-09-23
• [Presentation] マウス生殖細胞におけるSINE配列と宿主エピゲノムの相互作用 (2011)
  • Author(s): 一柳健司
  • Organizer: 第83回日本遺伝学会
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 2011-09-20
• [Presentation] Locus- and domain-dependent control of DNA methylation at mouse B1 retrotransposon during male germ-cell development (2011)
  • Author(s): Kenji Ichiyanagi, Yungfeng Li, Toshiaki Watanabe, Kei Fukuda, Junko Kitayama, Yoshiyuki Seki, Yukihiro Yabuta, Yasuhiro Yamamoto, Tomoko Ichiyanagi, Hiroyuki Sasaki
  • Organizer: Gordon Research Conference
  • Place of Presentation: 米国マサチュセッツ州
  • Year and Date: 2011-08-07
• [Presentation] SINE配列から見るマウス生殖細胞におけるDNAメチル化機構と転写制御機構 (2011)
  • Author(s): 一柳健司、李玉鳳、渡部聡朗、福田渓、北山淳子、関由行、薮田幸宏、山本耕裕、佐々木裕之
  • Organizer: 第5回日本エピジェネティクス研究会
  • Place of Presentation: 熊本
  • Year and Date: 2011-05-18
• [Presentation] SINE配列から見たマウス生殖細胞のエピゲノム制御機構 (2011)
  • Author(s): 一柳健司
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 京都
• [Presentation] マウス生殖細胞におけるSINE配列と宿主エピゲノムの相互作用 (2011)
  • Author(s): 一柳健司
  • Organizer: 第83回日本遺伝学会
  • Place of Presentation: 京都
• [Presentation] マウス精子形成過程におけるB1レトロトランスポゾンのDNAメチル化はpiRNAに依存せず、ローカス特異的に制御されている (2011)
  • Author(s): 一柳健司、李玉鳳、北山淳子、渡部聡朗、山本耕裕、佐々木裕之
  • Organizer: 日本遺伝学会第82回札幌大会
  • Place of Presentation: 札幌
• [Presentation] piRNA-independent, but genomic-location-dependent control of DNA methylation of mouse B1 retrotransposon during male germ-line development. (2011)
  • Author(s): 一柳健司、李玉鳳、北山淳子、渡部聡朗、山本耕裕、佐々木裕之
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Remarks] ホームページ
  • URL: http://www.bioreg.kyushu-u.ac.jp/labo/epigenome/index.html