The physiological role of SUMO ylation in mature photoreceptors

• Project/Area Number: 22770071
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Animal physiology/Animal behavior
• Research Institution: 公益財団法人大阪バイオサイエンス研究所 (2011); Osaka Bioscience Institute (2010)
• Principal Investigator: ONISHI Akishi 公益財団法人大阪バイオサイエンス研究所, 発生生物学部門, 研究員 (70569102)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 網膜 / 視細胞 / SUMO化 / 翻訳後修飾

Research Abstract

Post-translational modification(PTM) plays a crucial role in switching activities of the target proteins. Since SUMOylation, one of PTMs, is responsible for photoreceptor specification, we studied the functional role of SUMOylation of mature photoreceptors by using mice in which SUMO E2 ligase was conditionally disrupted. As a result, these conditional knockout mice were normal in immunohistochemical studies and electrophysiological recording, suggesting that SUMOylation is more important in specification and maturation, but not in phototransduction in photoreceptors.

Research Products

• [Journal Article] Analysis of transcriptional regulatory pathways of photoreceptor genes by expression profiling of the Otx2-deficient retina (2011)
  • Author(s): Omori, Y., Katoh, K., Sato, S., Muranishi, Y., Chaya, T., Onishi, A.(代表), Minami, T., Fujikado, T., Furukawa, T.
  • Journal Title: PLoS One Volume: 6(5) Issue: 5 Pages: e19685-e19685
  • DOI: 10.1371/journal.pone.0019685
  • Peer Reviewed
• [Journal Article] miR-124a is required for hippocampal axogenesis and retinal cone survival through Lhx2 suppression (2011)
  • Author(s): Sanuki R, Onishi A, Koike C, Muramatsu R, Watanabe S, Muranishi Y, Irie S, Uneo S, Koyasu T, Matsui R, Cherasse Y, Urade Y, Watanabe D, KondoM, Yamashita T, Furukawa T
  • Journal Title: Nat Neurosci Volume: 14 Issue: 9 Pages: 1125-1134
  • DOI: 10.1038/nn.2897
  • Peer Reviewed
• [Journal Article] Analysis of transcriptional regulatory pathways of photoreceptor genes by expression profiling of the Otx2-delicient retina (2011)
  • Author(s): Omori, Y., 6番目
  • Journal Title: PloS One Volume: 6
  • Peer Reviewed
• [Journal Article] Blimp1 suppresses Chx10 expression in differentiating retinal photoreceptor precursors to ensure proper photoreceptor development (2010)
  • Author(s): Katoh, K., Omori, Y., Onishi, A.(代表), Sato, S., Kondo, M., Furukawa, T.
  • Journal Title: J Neurosci Volume: 30(19) Issue: 19 Pages: 6515-6526
  • DOI: 10.1523/jneurosci.0771-10.2010
  • Peer Reviewed
• [Journal Article] The orphan nuclear hormone receptor ERR beta controls rod photoreceptor survival (2010)
  • Author(s): Onishi, A.(代表), Peng, G. H., Poth, E. M., Lee, D. A., Chen, J., Alexis, U., de Melo, J., Chen, S., Blackshaw, S.
  • Journal Title: Proc Natl Acad Sci USA Volume: 107(25) Issue: 25 Pages: 11579-11584
  • DOI: 10.1073/pnas.1000102107
  • Peer Reviewed
• [Journal Article] Pias3-dependent SUMOylation controls mammalian cone photoreceptor differentiation (2010)
  • Author(s): Onishi, A.(代表), Peng, GH., Chen, S., Blackshaw, S.
  • Journal Title: Nature Neurosci Volume: 13(9) Issue: 9 Pages: 1059-1065
  • DOI: 10.1038/nn.2618
  • Peer Reviewed
• [Journal Article] Blimp1 suppresses Chx10 expression in differentiating retinal photoreceptor precursors to ensure proper photoreceptor development (2010)
  • Author(s): Katoh, K., (3番目)
  • Journal Title: J, Neurosci. Volume: 30 Pages: 6515-6526
  • Peer Reviewed
• [Journal Article] The orphan nuclear hormone receptor ERR beta controls rod photoreceptor survival. (2010)
  • Author(s): Onishi, A.
  • Journal Title: Proc Natl Acad Sci USA Volume: 107 Pages: 11579-11584
  • Peer Reviewed
• [Journal Article] Pias3-dependent SUMOylation controls mammalian cone photoreceptor differentiation. (2010)
  • Author(s): Onishi, A.
  • Journal Title: Nature Neurosci. Volume: 13 Pages: 1059-1065
  • Peer Reviewed
• [Presentation] 網膜再生医療への機能ゲノム学的アプローチ (2011)
  • Author(s): 大西暁士
  • Organizer: 細胞再生医療研究会
  • Place of Presentation: 神戸
  • Year and Date: 2011-07-31
• [Presentation] Pias3-dependent SUMOylation directs photoreceptor subtype specification (2010)
  • Author(s): 大西暁士(代表)
  • Organizer: 日本神経科学学会2010年度年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-03
• [Presentation] Pias3-dependent SUMOylation directs photoreceptor subtype specification (2010)
  • Author(s): 大西暁士
  • Organizer: 日本神経科学学会2010年度年会
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2010-09-03