Very long chain ceramide biosynthesis and its molecular mechanism
| Project/Area Number |
22770095
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Structural biochemistry
|
|---|
| Research Institution | Nagoya University (2011)
Hokkaido University (2010) |
|---|
Principal Investigator |
MIZUTANI Yukiko 名古屋大学, 医学系研究科, COE 特任助教 (30396296)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 脂質 / セラミド / スフィンゴ脂質 / 極長鎖脂肪酸 / 酵素 / バリア機能 / 細胞膜 / 皮膚機能 |
|---|
| Research Abstract |
The lipid lamellae in the stratum corneum is important for the epidermal permeability barrier. The lipid lamellae component ceramide, comprising an ultra long-chain fatty acid of >=26 carbons, plays an essential role in barrier formation. Ultra long-chain acyl-CoAs, produced by the FA elongase (ELOVL), are converted to ultra long-chain ceramides by the ceramide synthase (CERS). In the keratinocyte differentiation system, we observed that these mRNAs and proteins expression increased. We also determined that peroxisome proliferator-activated receptor is involved in the up-regulation of the mRNAs. Knockdown of CERS caused a reduction in the elongase activities toward ultra long-chain acyl-CoAs. These results are implying the possibility that ceramide synthasepositively regulates ultra long-chain fatty acid production. We also examined the possibility that the PPAR activation of ceramide can regulate the production of ceramides.
|
Report
(3 results)
Research Products
(17 results)
