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Structural analysis of pore-forming CEL-III complex

Research Project

Project/Area Number 22770105
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Structural biochemistry
Research InstitutionNagasaki University

Principal Investigator

UNNO Hideaki  長崎大学, 大学院・工学研究科, 助教 (10452872)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsCEL-III / 結晶構造解析 / PFT / X線結晶構造解析 / 膜孔形成毒素 / レクチン / Cucumaria echinata
Research Abstract

For structural analysis of pore forming CEL-III complex, enough quality of an electron density maps to build the complex model were made by phase calculation using X-ray diffraction data of native and heavy atom derivatives

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (15 results)

All 2011 2010

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (10 results)

  • [Journal Article] Covalent modification of reduced flavin mononucleotide in type-2 isopentenyl diphosphate isomerase by active-site-directed inhibitors2011

    • Author(s)
      T Nagai, H Unno, MW Janczak, T Yoshimura, CD Poulter, H Hemmi
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 108(51) Pages: 20461-20466

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Covalent modification of reduced flavin mononucleotide in type-2 isopentenyl diphosphate isomerase by active-site-directed inhibitors2011

    • Author(s)
      T Nagai, H Unno, MW Janczak, T Yoshimura, CD Poulter, H Hemmi
    • Journal Title

      Proc.Natl.Acad.Sci.USA.

      Volume: 108(51) Pages: 20461-20466

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Galactose recognition by a tetrameric C-type lectin, CEL-IV, containing the EPN carbohydrate-recognition motif.2011

    • Author(s)
      Tomomitsu Hatakeyama, Takuro Kamiya, Masami Kusunoki, Sachiko Nakamura-Tsuruta, Jun Hirabayashi, Shuichiro Goda, Hideaki Unno
    • Journal Title

      J.Biol.Chem.

      Volume: 286(12) Pages: 10305-10315

    • NAID

      120006985160

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of tetraketide lactones by mutated Antirrhinum majus chalcone synthases (AmCHS1)2010

    • Author(s)
      Masayoshi Hatayama, Hideaki Unno, Masami Kusunoki, Seiji Takahashi, Tokuzo Nishino, Toru Nakayama
    • Journal Title

      J.Biosci.Bioeng.

      Volume: 110(2) Pages: 158-164

    • NAID

      110007700563

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The role and structure of the carboxyl-terminal domain of the human voltage-gated proton channel Hv1.2010

    • Author(s)
      Shu Jie Li, Qing Zhao, Qiangjun Zhou, Hideaki Unno, Yujia Zhai, Fei Sun
    • Journal Title

      J.Biol.Chem.

      Volume: 285(16) Pages: 12047-12054

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 超好熱アーキア由来膜タンパク質の緑色蛍光タンパク質融合体としての生産2011

    • Author(s)
      眞方山慶佑, 海野英昭, 畠山智充, 郷田秀一郎
    • Organizer
      日本農芸化学会西日本支部大会
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 糖鎖付加デンドリマーを用いたレクチン活性測定法の開発2011

    • Author(s)
      狩野良太, 海野英昭, 郷田秀一郎, 畠山智充
    • Organizer
      日本農芸化学会西日本支部大会
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 糖結合部位に変異を導入したC型レクチンCEL-IVの糖結合特異性と立体構造の解明2011

    • Author(s)
      本多麻美, 海野英昭, 郷田秀一郎, 畠山智充
    • Organizer
      日本農芸化学会西日本支部大会
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] C型レクチンCEL-Iの糖結合部位アミノ酸置換による結合特異性の変化2011

    • Author(s)
      馬場智大, 海野英昭, 郷田秀一郎, 畠山智充
    • Organizer
      日本農芸化学会西日本支部大会
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Cucumaria echinata由来溶血性レクチンCEL-III膜孔形成複合体の結晶化2010

    • Author(s)
      海野英昭, 竪谷勇樹, 郷田秀一郎, 畠山智充
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] 超好熱アーキアPyrococcus horikoshii由来PHO233産物の遺伝子組換え発現系の構築と可溶化条件の検討2010

    • Author(s)
      松原悠太, 久松啓伍, 海野英昭, 畠山智充, 郷田秀一郎
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 超好熱アーキアSulfolobus tokodaii由来ST1670産物の遺伝子組換え発現系の構築と可溶化条件の検討2010

    • Author(s)
      池田光司, 福田泰信, 真方山慶佑, 海野英昭, 畠山智充, 郷田秀一郎
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 超好熱アーキア由来糖結合タンパク質の探索2010

    • Author(s)
      栗浦良, 海野英昭, 畠山智充, 郷田秀一郎
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] タイプ2イソペンテニル二リン酸イソメラーゼの反応機構;還元型FMNの触媒機構2010

    • Author(s)
      長井拓也, 海野英昭, 吉村徹, 邊見久
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] 海産無脊椎動物レクチンCEL-IIIの溶血活性発現に関与するアミノ酸残基の同定2010

    • Author(s)
      久松啓伍、海野英昭、郷田秀一郎、畠山智充
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2010-06-18
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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