An electron microscopic study of actin nucleation factors
| Project/Area Number |
22770145
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | アクチン / 電子顕微鏡 / 電子線トモグラフィー / 細胞骨格 / 画像解析 / Arp2/3複合体 / フォルミン / 三次元構造 / フィラメント極性 / 単粒子解析 / 重合核形成 / トレッドミリング |
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| Research Abstract |
The actin filament is responsible for a wide range of movement of the cell and dynamic through construction and destruction. Many proteins regulate the dynamics of the actin filament. Arp2/3 complex and formin are ones of the regulatory proteins and they start the construction of the actin filament in the cell. To understand mechanism of starting construction, we developed structural analysis procedures of the actin filament network in the cell with an Austrian group, which determined distribution and structure of Arp2/3 complex in the cell by using electron microscopy and image analysis. We also elucidated that formin binds only to the ends of the actin filament, not the side. Now we are analyzing data to conform the bound end to formin is the barbed end.
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Report
(3 results)
Research Products
(46 results)
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[Journal Article] A novel actin-like filament structure from Clostridium tetani2012
Author(s)
Popp, D., Narita, A., Lee, LJ., Ghoshdastider, U., Xue, B., Srinivasan, R., Balasubramanian, MK., Tanaka, T., Robinson, RC.
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Journal Title
J. Biol. Chem.
Volume: (accepted)
Related Report
Peer Reviewed
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[Presentation] Two distinct mechanisms for actin capping protein regulation EMBO Lecture Courese The 26th2011
Author(s)
Takeda S., Minakata S., Koike R., Kawahata I., Narita A., Kitazawa M., Ota M., Yamakuni T., Maeda Y., Nitanai Y.
Organizer
European Cytoskeletal Forum Meeting "Actin-Based Motility
Place of Presentation
Hotel Palma(Stresa,-Lake Maggiore)
Year and Date
2011-10-30
Related Report
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[Presentation] Two distinct mechanisms for actin capping protein regulation2011
Author(s)
Takeda S., Minakata S., Koike R., Kawahata I., Narita A., Kitazawa M., Ota M., Yamakuni T., Maeda Y., Nitanai Y.
Organizer
EMBO Lecture Courese The 26th.European Cytoskeletal Forum Meeting "Actin-Based Motility"
Place of Presentation
Hotel Palma (Stresa,-Lake Maggiore)
Year and Date
2011-10-30
Related Report
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