| Project/Area Number |
22770169
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | Yokohama National University |
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Principal Investigator |
SUZUKI Atsushi 横浜国立大学, 学際プロジェクト研究センター, 特任教員(助教) (60467058)
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| Co-Investigator(Renkei-kenkyūsha) |
SAGA Yumiko 国立遺伝学研究所, 系統生物センター発生工学研究室, 教授 (50221271)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | RNA / 生殖細胞 / 発生 / マウス |
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| Research Abstract |
In our present study, we identify CNOT1, a component of the CCR4-NOT deadenylation complex, as a direct factor mediating the interaction with NANOS2. We find that the first 10 amino acids(AAs) of NANOS2 are required for this binding. We further observe that a NANOS2 mutant lacking these first 10 AAs(NANOS2-ΔN10) fails to rescue defects in the Nanos2-null mouse. Our current data thus indicate that the interaction with the CCR4-NOT deadenylation complex is essential for NANOS2 function.
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