Analysis of the early steps of DNA damage response through 9-1-1/TopBP1 interaction
| Project/Area Number |
22770172
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
OHASHI Eiji 九州大学, 理学研究院, 助教 (90378951)
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| Research Collaborator |
TSURIMOTO Toshiki 九州大学, 理学研究院, 教授 (30163885)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | DNA損傷応答 / チェックポイント / 蛋白質問相互作用 / 細胞内局在 / 試験管内再構築 / DNA損傷・修復 / タンパク質間相互作用 |
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| Research Abstract |
The Rad9Husl-Radl(9-1-1) complex, a DNA damage sensor interacts with TopBP1 to activate a signaling pathway. In this study, we found that 911/TopBP1 interaction plays an important role in the signal transduction after their localization to the sites of DNA damage without affecting their localization upon DNA damage. The binding ability of Rad9 to TopBP1 was strongly correlated with the activity of DNA damage response in human cells. Finally, our in vitro results suggest that 9-1-1 has a potential to interact with DNA and the binding ability may be regulated by the interaction with other factors.
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Report
(3 results)
Research Products
(24 results)
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[Journal Article] The vital role of polymerase C and REV1 in mutagenic, but not correct, DNA synthesis across benzo[ a] pyrene-dG and recruitment of polymerase C by REV1 to replicationstalled site2012
Author(s)
Hashimoto K, Cho Y, Yang IY, Akagi J, Ohashi E, Tateishi S, de Wind N, Hanaoka F, Ohmori H, and Moriya M.
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Journal Title
J. Biol. Chem
Volume: 287
Pages: 9613-9622
Related Report
Peer Reviewed
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