Toward the understanding of the switching mechanism between DNA checkpo i nt and repa i r mach i neries at DNA damage s i tes.

• Project/Area Number: 22770178
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: Kyoto University (2011); National Institute of Genetics (2010)
• Principal Investigator: FURUYA Kanji 京都大学, 放射線生物研究センター, 講師 (90455204)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: DNA損傷 / DNA修復 / 細胞周期細胞周期 / チェックポイント / リン酸化 / Rad9 / DDK

Research Abstract

Genomic DNA where our genetic information were encoded was easily damaged by irradiation by gamma-ray or ultraviolet. These damaged DNA were not transferred to daughter cells until they get repaired. Upon repairing DNA damage must be quickly sensed by checkpoint mechanisms and correctly fixed through the repairing enzymes. These two processes, checkpoint and repair mechanisms, must be tightly cooperated, but the switching mechanism is undefined. Here we unveil that phosphorylation dependent feedback mechanism that is responsible the switching.

Research Products

• [Journal Article] Construction of an insertion marker collection of Sz. japonicus(IMACS) for genetic mapping and a fosmid library covering its genome (2012)
  • Author(s): Furuya, K. Aoki, K. and Niki, H
  • Journal Title: Yeast Volume: 5 Issue: 6 Pages: 241-249
  • DOI: 10.1002/yea.2907
  • Peer Reviewed
• [Journal Article] Diploid construction by interallelic complementation in Schizosaccharomyces japonicus (2011)
  • Author(s): Furuya, K., andNiki, H.
  • Journal Title: Yeast Volume: 28 Issue: 10 Pages: 747754-747754
  • DOI: 10.1002/yea.1898
  • Peer Reviewed
• [Journal Article] Breakage of the nuclear envelope by an extending mitotic nucleus occurs during anaphase in Schizosaccharomyces japonicus. (2011)
  • Author(s): Aoki K. et al.
  • Journal Title: Genes to Cells Volume: 16 Issue: 9 Pages: 911-926
  • DOI: 10.1111/j.1365-2443.2011.01540.x
  • Peer Reviewed
• [Journal Article] Comparative functional genomics of the fission yeast (2011)
  • Author(s): Rhino, N., Chen, Z., Yassour, M., Thompson, D. A., Haas, B. J., Habib, N., Wapinski, I., Roy, S., Lin, M. F., Herman, D. I., Young, S. K., Furuya, K., Guo, Y(計47名) Nusbaum, C.
  • Journal Title: Science 332 Volume: 332 Issue: 6032 Pages: 930-936
  • DOI: 10.1126/science.1203357
  • Peer Reviewed
• [Journal Article] The DNA damage checkpoint regulates a transition between yeast and hyphal growth in Schizosaccharomyces japonicus (2010)
  • Author(s): Q Furuya, K., and Niki, H.
  • Journal Title: Mol. Cel. Biol Volume: 30 Issue: 12 Pages: 2909-2917
  • DOI: 10.1128/mcb.00049-10
  • Peer Reviewed
• [Journal Article] DDK phosphorylates checkpoint clamp component Rad9 and promotes its release from damage chromatin (2010)
  • Author(s): 古谷寛治, et al.
  • Journal Title: Molecular Cell Volume: 40 Pages: 606-618
  • Peer Reviewed
• [Journal Article] Novel episomal vectors and a high efficient transformation procedure for the fission yeast Schizosaccharomyces japonicus (2010)
  • Author(s): 青木敬太, et al.
  • Journal Title: Yeast Volume: 27 Pages: 1049-1060
  • Peer Reviewed
• [Journal Article] The DNA damage checkpoint regulates a transition between yeast and hyphal growth in Schizosaccharomyces japonicus (2010)
  • Author(s): 古谷寛治、仁木宏典
  • Journal Title: Molecular and Cellular Biology Volume: 30 Pages: 2909-2917
  • Peer Reviewed
• [Presentation] The regulation of checkpoint proteins by checkpoint kinase ATR and DNA replication kinase DDK BMB2011 (2011)
  • Author(s): 古谷寛治
  • Organizer: (日本分子生物学会年会)ワークショップ
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] The regulation of checkpoint proteins by checkpoint kinase ATR and DNA replication kinase DDK (2011)
  • Author(s): 古谷寛治
  • Organizer: 日本分子生物学会年会・ワークショップ
  • Place of Presentation: 神奈川県横浜市、パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] REGULATION OF DNA DAMAGE CHECKPOINT SIGNALLING THROUGH PHOSPHORYLATION (2011)
  • Author(s): K Furuya
  • Organizer: The 27^<th> RBC-NIRS International Symposium
  • Place of Presentation: Kyoto
  • Year and Date: 2011-12-09
• [Presentation] REGULATION OF DNA DAMAGE CHECKPOINT SIGNALLING THROUGH PHOSPHORYLATION (2011)
  • Author(s): 古谷寛治
  • Organizer: The 27th RBC-NIRS International Symposium
  • Place of Presentation: 京都市、コープイン京都(招待講演)
  • Year and Date: 2011-12-09
• [Presentation] チェックポイントタンパク質のDDKキナーゼ依存的な制御機構 (2011)
  • Author(s): 白岩善治、仁木宏典、古谷寛治
  • Organizer: 第21回「DNA複製・組換え・ゲノム安定性制御ワークショップ」
  • Place of Presentation: 福岡
  • Year and Date: 2011-10-26
• [Presentation] チェックポイントタンパク質のDDKキナーゼ依存的な制御機構 (2011)
  • Author(s): 古谷寛治
  • Organizer: 第21回「DNA複製・組換え・ゲノム安定性制御ワークショップ」
  • Place of Presentation: 福岡県福津市、サンピア福岡
  • Year and Date: 2011-10-25
• [Presentation] 複製キナーゼDDKに依るチェックポイントタンパク質の制御 (2011)
  • Author(s): 古谷寛治
  • Organizer: 第1回ゲノム損傷応答研究会
  • Place of Presentation: 静岡県三島市国立遺伝学研究所
  • Year and Date: 2011-10-16
• [Presentation] 複製キナーゼDDKに依るチェックポイントタンパク質の制御 (2011)
  • Author(s): 古谷寛治
  • Organizer: ゲノム損傷応答研究会
  • Place of Presentation: 静岡県三島市、国立遺伝学研究所
  • Year and Date: 2011-10-15
• [Presentation] DDK phosphorylates checkpoint clamp component Rad9 and promotes its release from damaged chromatin Cold Spring Harbour Meeting, Eukaryotic Replication & Genome Maintenance (2011)
  • Author(s): Kanji Furuya, Izumi Miyabe, Naoko Kakusho, Hisao Masai, Hironori Niki, Antony M Carr
  • Organizer: Cold Spring Harbour Meeting, Eukaryotic Replication & Genome Maintenance
  • Place of Presentation: Cold Spring Harbour
  • Year and Date: 2011-09-25
• [Presentation] DDK phosphorylates checkpoint clamp component Rad9 and promotes its release from damaged chromatin (2011)
  • Author(s): Kan. ji Furuya, Izumi Miyabe, Naoko Kakusho, Hisao Masai, Hironori Niki, Antony M Carr
  • Organizer: The 6th Fission Yeast Meeting
  • Place of Presentation: Boston
  • Year and Date: 2011-09-09
• [Presentation] 分裂酵母RAD9のDDK依存的なリン酸化の機能 (2011)
  • Author(s): 古谷寛治
  • Organizer: 染色体ワークショップ
  • Place of Presentation: 石川県加賀市瑠璃光
  • Year and Date: 2011-01-11
• [Presentation] DDK promotes checkpoint clamp Rad9 and promotes its release from damaged chromatin (2010)
  • Author(s): 古谷寛治
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピアランド
  • Year and Date: 2010-12-08
• [Presentation] DDK phosphorylates checkpoint clamp Rad9 and promotes its release from damaged Chromatin (2010)
  • Author(s): Furuya, K., Miyabe, I., Tsutsui, Y., Kakusho, N., Masai, H., Carr AM., Niki, H.
  • Organizer: 33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] DDK promotes checkpoint clamp Rad9 and promotes its release from damaged chromatin (2010)
  • Author(s): 古谷寛治
  • Organizer: The 7^<th> 3R symposium
  • Place of Presentation: 富山カンファレンスセンター(招待講演)
  • Year and Date: 2010-10-29
• [Presentation] DDK phosphorylates checkpoint clamp Rad9 and promotes its release from damaged chromatin. (2010)
  • Author(s): Furuya, K., , Miyabe, I., Tsutsui, Y., Kakusho, N., Masai, H., Paderi F., Carr AM., Niki, H.
  • Organizer: 7th 3R symposium
  • Place of Presentation: Toyama
  • Year and Date: 2010-10-27
• [Presentation] DDK phosphorylates checkpoint clamp Rad9 and promotes its release from damaged Chromatin (2010)
  • Author(s): Furuya, K., Miyabe, I., Tsutsui, Y., Paderi F., Kakusho, N., Masai, H., Carr AM., Niki, H.
  • Organizer: 第53回放射線影響学会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-21
• [Presentation] DDK phosphorylates checkpoint clamp Rad9 and promotes its release from damaged Chromatin. (2010)
  • Author(s): Furuya, K., Miyabe, I., Tsutsui, Y., Kakusho, N., Masai, H., Carr AM., Niki, H.
  • Organizer: The 57th NIBB conference
  • Place of Presentation: Okazaki
  • Year and Date: 2010-10-15
• [Presentation] DDK phosphorylates checkpoint clamp Rad9 and promotes its release from damaged Chromatin (2010)
  • Author(s): Furuya, K., Tsutsui, Y., Miyabe, I., Kakusho, N., Carr AM., Masai, H., Niki, H.
  • Organizer: EMBO workshop
  • Place of Presentation: Freiburg
  • Year and Date: 2010-07-22
• [Presentation] DDK phosphorylates checkpoint clamp Rad9 and promotes its release from damaged chromatin. (2010)
  • Author(s): Furuya, K., Miyabe, I., Kakusho, N., Masai, H., Carr AM., Niki, H.
  • Organizer: International Conference on Radiation and Cancer Biology
  • Place of Presentation: Nagasaki
  • Year and Date: 2010-02-18
• [Remarks] ホームページ等成果は随時更新している。
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