Ubiquitination mediated autophagy against invading bacteria

• Project/Area Number: 22770194
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: FUJITA Naonobu 大阪大学, 生命機能研究科, 助教 (00506496)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: オートファジー / ユビキチン / タンパク質分解

Research Abstract

Although ubiquitination is thought to be important for the autophagic response to invading bacteria(also called xenophagy), it remains enigmatic whether ubiquitin is conjugated to bacterial proteins or host cellular proteins. Here we found that rupture of endosome by bacterial toxin or transfection reagent leads to ubiquitination of host cellular endosomal proteins, and that is sufficient for induction of selective autophagy.

Research Products

• [Journal Article] Autophagy guards against cisplatin-induced acute kidney injury (2012)
  • Author(s): Takahashi A, Kimura T, Takabatake Y, Namba T, Kaimori J, Kitamura H, Matsui I, Niimura F, Matsusaka T, Fujita N, Yoshimori T, Isaka Y, Rakugi H.
  • Journal Title: Am. J. Pathol Volume: 180 Pages: 517-525
  • Peer Reviewed
• [Journal Article] バクテリアの侵入時にみられるユビキチン化とAtg蛋白質の局在化機構 (2012)
  • Author(s): 藤田尚信、吉森保
  • Journal Title: 炎症と免疫 Volume: 20 Pages: 38-42
• [Journal Article] Autophagy guards against cisplatin-induced acute kidney injury (2012)
  • Author(s): Takahashi A, Kimura T, Takabatake Y, Namba T, Kaimori J, Kitamura H, Matsui I, Niimura F, Matsusaka T, Fujita N.Yoshimori T, Isaka Y, Rakugi H
  • Journal Title: Am.J.Pahol. Volume: 180(2) Pages: 517-525
  • Peer Reviewed
• [Journal Article] Atg16L2, a novel isoform of mammalian Atg16L that is not essential for canonical autophagy despite forming an Atg12-5-16L2 complex (2011)
  • Author(s): Ishibashi K, Fujita N, Kanno E, Omori H, Yoshimori T, Itoh T, Fukuda M.
  • Journal Title: Autophagy Volume: 7 Pages: 1500-1513
  • Peer Reviewed
• [Journal Article] Dysfunction of autophagy participates in vacuole formation and cell death in cells replicating hepatitis C virus (2011)
  • Author(s): Taguwa S, Kambara H, Fujita N, Noda T, Yoshimori T, Koike K, Moriishi K, Matsuura Y.
  • Journal Title: J. Virol Volume: 86 Pages: 13185-94
  • Peer Reviewed
• [Journal Article] Ubiquitination-Mediated Autophagy Against Invading Bacteria (2011)
  • Author(s): Fujita N and Yoshimori T.
  • Journal Title: Curr. Opin. Cell Biol Volume: 4 Pages: 492-497
  • Peer Reviewed
• [Journal Article] オートファジー～分子機構と疾患との関わり～ (2011)
  • Author(s): 藤田尚信、吉森保
  • Journal Title: BIOCLINICA Volume: 26 Pages: 369-372
• [Journal Article] Inhibition of autophagy potentiates the antitumor effect of the multikinase inhibitor sorafenib in hepatocellular carcinoma (2011)
  • Author(s): Shimizu S, Takehara T, Hikita H, Kodama T, Tsunematsu H, Miyagi T, Hosui A, Ishida H, Tatsumi T, Kanto T, Hiramatsu N, Fujita N, Yoshimori T, Hayashi N
  • Journal Title: Int.J.Cancer Volume: 131 Issue: 3 Pages: 548-57
  • DOI: 10.1002/ijc.26374
  • Peer Reviewed
• [Journal Article] Dysfunction of autophagy participates in vacuole formation and cell death in cells replicating hepatitis C virus (2011)
  • Author(s): Taguwa S., Kambara H., Fujita N., Noda T., Yoshimori T., Koike K., Moriishi K., and Matsuura Y.
  • Journal Title: J. Virol Volume: 85 Issue: 24 Pages: 13185-13194
  • DOI: 10.1128/jvi.06099-11
  • Peer Reviewed
• [Journal Article] Atg16L2, a novel isoform of mammalian Atgl6L that is not essential for canonical autophagy despite forming an Atg12-5-16L2 complex (2011)
  • Author(s): Ishibashi K, Fujita N.Kanno E, Omori H, Yoshimori T, Itoh T, Fukuda M
  • Journal Title: Autophagy Volume: 7(12) Pages: 1500-1513
  • Peer Reviewed
• [Journal Article] Ubiquitination-Mediated Autophagy Against Invading Bacteria (2011)
  • Author(s): Fujita N, Yoshimori T.
  • Journal Title: Curr.Opin.Cell Biol. Volume: (掲載確定・印刷中)
  • Peer Reviewed
• [Journal Article] Autophagosomes can support Yersinia pseudotuberculosis replication in macrophages (2010)
  • Author(s): Moreau K, Lacas-Gervais S, Fujita N, Sebbane F, Yoshimori T, Simonet M, Lafont F.
  • Journal Title: Cell Microbiol Volume: 12 Pages: 1108-1123
  • Peer Reviewed
• [Journal Article] Combinational soluble N-ethylmaleimide-sensitive factor attachment protein receptor proteins VAMP8 and Vti1b mediate fusion of antimicrobial and canonical autophagosomes with lysosomes (2010)
  • Author(s): Furuta N, Fujita N, Noda T, Yoshimori T, Amano A.
  • Journal Title: Mol. Biol. Cell Volume: 21 Pages: 1001-1010
  • Peer Reviewed
• [Journal Article] Combinational soluble N-ethylmaleimide-sensitive factor attachment protein receptor proteins VAMP8 and Vtilb mediate fusion of antimicrobial and canonical autophagosomes with lysosomes. (2010)
  • Author(s): Furuta N, Fujita N, Noda T, Yoshimori T, Amano A.
  • Journal Title: Mol.Biol.Cell Volume: 21 Pages: 1001-1010
  • Peer Reviewed
• [Journal Article] Autophagosomes can support Yersinia pseudotuberculosis replication in macrophages. (2010)
  • Author(s): Moreau K, Lacas-Gervais S, Fujita N, Sebbane F, Yoshimori T, Simonet M, Lafont F.
  • Journal Title: Cell Microbiol. Volume: 12 Pages: 1108-1123
  • Peer Reviewed
• [Presentation] Ubiq uitination-mediated selective autophagy (2011)
  • Author(s): Naonobu Fujita, Megumi Nakaoka, Kage yama Shun, Yuuki Osada, Eiji Morita, Ta keshi Noda, and Tamotsu Yoshimori
  • Organizer: 日本生化学会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-24
• [Presentation] Selective Autophagy and Ubiquitination accompanying Endosomal Damage (2011)
  • Author(s): Naonobu Fujita, Megumi Nakaoka, Kageyama Shun, Yuuki Osada, Eiji Morita, Takeshi Noda, Tamotsu Yoshimori
  • Organizer: 日本生化学会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2011-09-24
• [Presentation] Ubiquitination-mediated selective autophagy (2011)
  • Author(s): Naonobu Fujita, Megumi Nakaoka, Kageyama Shun, Yuuki Osada, Eiji Morita, Takeshi Noda, Tamotsu Yoshimori
  • Organizer: 内藤カンファレンス
  • Place of Presentation: 札幌シャトーキングダム
  • Year and Date: 2011-06-29
• [Presentation] 感染微生物に対するオートファジーの分子機構 (2010)
  • Author(s): 藤田尚信、吉森保
  • Organizer: Biochemistry and Molecular Biology 2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] 感染微生物に対するオートファジーの分子機構 (2010)
  • Author(s): 藤田尚信、吉森保
  • Organizer: Biochemistry and Molecular Biology 2010
  • Place of Presentation: 神戸ポートアイランド(招待講演)
  • Year and Date: 2010-12-09
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/yoshimori/jp/achievement/010/
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/yoshimori/jp/index.html
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/yoshimori/jp/achievement/010/