Mechanisms and roles of cell binucleation

• Project/Area Number: 22770196
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Gakushuin University
• Principal Investigator: TANIGUCHI Kiichiro 学習院大学, 理学部生命科学科, 助教 (20554174)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ショウジョウバエ / 二核細胞 / 生殖器附属腺 / 細胞分裂 / 細胞周期 / 細胞二核化 / 中心紡錘体 / Mud / NuMA / 多核細胞 / 有糸分裂 / 細胞質分裂 / 収縮環

Research Abstract

Some cells such as myocytes and hepatocytes can contain two nuclei. In this research project, I examined mechanisms and roles of the binucleation using the Drosophila tissue. In the result, we identified Mud which is a key factor to control the behavior of binucleating cells. We also found that binucleate state enables a cell to effectively change its shape by rearrangement of two nuclei for a plasticity of organ shape.

Research Products

• [Journal Article] Reduced cell number in the hindgut epithelium disrupts hindgut left-right asymmetry in a mutant of pebble, encording a RhoGEF, in Drosophila embryos (2013)
  • Author(s): Mitsutoshi Nakamura, Kenjiroo Matsumoto, Yuta Iwamoto, Takeshi Muguruma, Naotaka Nakazawa, Ryo Hatori, Kiichiro Taniguchi, Reo Maeda, and Kenji Matsuno
  • Journal Title: Mechanisms of Development Volume: 130 Issue: 2-3 Pages: 169-180
  • DOI: 10.1016/j.mod.2012.09.007
  • Peer Reviewed
• [Journal Article] Canonical Wnt signaling in the visceral muscle is required for left-right asymmetric development of the Drosopbila midgut (2012)
  • Author(s): Junpei Kuroda, Mitsutoshi Nakamura, Masashi Yoshida, Haruka Yamamoto, Takaaki Maeda, Kiichiro Taniguchi, Naotaka Nakazawa, Ryo Hatori, Akira Ishio, Ayumi Ozaki, Shunsuke Shimaoka, Tamiko Ito, Hironao Iida, Takashi Okumura, Reo Maeda, Kenji Matsuno
  • Journal Title: Mechanisms of Development Volume: 128 Issue: 11-12 Pages: 625-639
  • DOI: 10.1016/j.mod.2011.12.002
  • Peer Reviewed
• [Journal Article] The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila. (2012)
  • Author(s): Taniguchi, K., Maeda, R., Ando, T., Okumura, T., Minami, R., Wakabayashi, M., Sugimori, S., Taniguchi, K., Kokuryo, A., Imano, T., Adachi-Yamada, T., Watanabe, N. and Nakagoshi, H.
  • Journal Title: PLoS One Volume: 7 Pages: 32302-32302
  • Peer Reviewed
• [Journal Article] A novel Cre/loxP system for mosaic gene expression in the Drosophila embryo (2012)
  • Author(s): Naotaka Nakazawa, Kiichiro Taniguchi, Takashi Okumura, Reo Maeda, Kenji Matsuno
  • Journal Title: Developmental Dynamics Volume: 241 Issue: 5 Pages: 965-974
  • DOI: 10.1002/dvdy.23784
  • Peer Reviewed
• [Journal Article] The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila. (2012)
  • Author(s): Minami, R.
  • Journal Title: PLoS One Volume: 7 Issue: 3 Pages: e32302-e32302
  • DOI: 10.1371/journal.pone.0032302
  • Peer Reviewed
• [Journal Article] Binucleation of Drosophila adult male accessory gland cells increases plasticity of organ size for effective reproduction. (2011)
  • Author(s): Taniguchi, K., Kokuryo, A., Imano, T., Minami, R., Nakagoshi, H. and Adachi-Yamada, T.
  • Journal Title: Biological Systems- Open Access Volume: 1 Issue: 01 Pages: 101-101
  • DOI: 10.4172/bso.1000e101
• [Journal Article] Chirality in Planar cell shape contributes to left-right asymmetric epithelial morphogenesis (2011)
  • Author(s): Kiicniro Tanigucni, Reo Maeda, Tadashi Ando, Takashi Okumura, Naotaka Nakazawa, Ryo Hatori, Mitsutoshi Nakamura, Shunya Hozumi, Hiroo Fujiwara, Kenji Matsuno
  • Journal Title: Science Volume: 333 Issue: 6040 Pages: 339-341
  • DOI: 10.1126/science.1200940
  • Peer Reviewed
• [Journal Article] Binucleation of Drosophila Adult Male Accessory Gland Cells Increases Plasticity of Organ Size for Effective Reproduction (2011)
  • Author(s): Kiichiro Taniguchi
  • Journal Title: Journal of Organ Biology Volume: 1
• [Journal Article] Left-right asymmetric morphogenesis of the anterior midgut depends on the activation of a non-muscle myosin II in Drosophila (2010)
  • Author(s): Okumura, T., Fujiwara, H., Taniguchi, K., Kuroda, J., Nakazawa, N., Nakamura, M., Hatori, R., Ishio, A., Maeda, R. and Matsuno, K.
  • Journal Title: Developmental Biology Volume: 344 Issue: 2 Pages: 693-706
  • DOI: 10.1016/j.ydbio.2010.05.501
  • Peer Reviewed
• [Presentation] Elimination of malfunctioning cells in Drosophila male accessory gland, a non-regenerative tissue. (2012)
  • Author(s): Taniguchi, K., Abe, S., Minami, R., Imano, T., Kokuryo, A., Nakagoshi, H. and Adachi-Yamada, T.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
  • Year and Date: 2012-12-12
• [Presentation] Cytokinesisdeficient binucleation in Drosophila accessory gland for providing plasticity of organ size. (2012)
  • Author(s): Taniguchi, K., Kokuryo, A., Imano, T., Sakata, R., Minami, R., Nakagoshi, H. and Adachi-Yamada, T.
  • Organizer: 53rd Annual Drosophila Research Conference
  • Place of Presentation: Sheraton Chicago Hotel & Towers (米国)
• [Presentation] Cytokinesis-deficient binucleation in Drosophila accessory gland for providing plasticity of organ size (2012)
  • Author(s): Kiichiro Taniguchi
  • Organizer: 53rd Annual Drosophila Research Conference
  • Place of Presentation: Sheraton Chicago Hotel & Towers, Illinois, U.S.A
• [Presentation] Cytokinesisdeficient binucleation in Drosophila accessory gland for providing a higher level of plasticity of organ size. (2011)
  • Author(s): Taniguchi, K., Kokuryo, A., Imano, T., Sakata, R., Minami, R., Nakagoshi, H. and Adachi-Yamada, T.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] Cytokinesis-deficient binucleation in Drosophila accessory gland for providing a higher level of plasticity of organ size (2011)
  • Author(s): Kiichiro Taniguchi
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜、神奈川県、日本
  • Year and Date: 2011-12-16
• [Presentation] Cytokinesisdeficient binucleation in Drosophila accessory gland for providing plasticity of organ size. (2011)
  • Author(s): Taniguchi, K., Kokuryo, A., Imano, T., Sakata, R., Minami, R., Nakagoshi, H. and Adachi-Yamada, T.
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2011-06-18
• [Presentation] Cytokinesis-deficient binucleation in Drosophila accessory gland for providing plasticity of organ size (2011)
  • Author(s): Kiichiro Taniguchi
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学、北海道、日本
  • Year and Date: 2011-06-18
• [Presentation] Cytokinesisdeficient binucleation in Drosophila accessory gland for providing plasticity of organ size. (2011)
  • Author(s): Taniguchi, K., Kokuryo, A., Imano, T., Sakata, R., Minami, R., Nakagoshi, H. and Adachi-Yamada, T.
  • Organizer: 1st Asia-Pacific Drosophila Research Conference
  • Place of Presentation: 劍潭青年活動中心 (台湾)
• [Presentation] Cytokinesis- deficient binucleation as a strategy for tissue enlargement in Drosophila. (2010)
  • Author(s): Taniguchi, K., Kokuryo, A., Imano, T., Minami, R., Nakagoshi, H. and Adachi-Yamada, T.
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] Cytokinesis-deficient binucleation as a strategy for tissue enlargement in Drosophila (2010)
  • Author(s): Kiichiro Taniguchi
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド兵庫県、日本
  • Year and Date: 2010-12-10
• [Presentation] Mud/NuMAはショウジョウバエ雄生殖器附属腺において細胞二核化を制御する
  • Author(s): 谷口 喜一郎
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: ウインクあいち
• [Book] 実験医学 (2012)
  • Author(s): 中澤直高、谷口喜一郎、前田礼男、安藤格士、松野健治
  • Publisher: 羊土社
• [Book] 細胞工学 (2011)
  • Author(s): 前田礼男、谷口喜一郎、安藤格士、松野健治
  • Publisher: 学研メディカル秀潤社
• [Remarks]
  • URL: http://www-cc.gakushuin.ac.jp/~e090001/
• [Remarks] 学習院大学・安達研究室
  • URL: http://www-cc.gakushuin.ac.jp/~e090001/index.html
• [Remarks]
  • URL: http://www-cc.gakushuin.ac.jp/~e090001/index.html
• [Remarks]
  • URL: http://www-cc.gakushuin.ac.jp/~e090001/index.html