A study of molecular mechanisms of assembly of the nuclear envelope-like membrane using DNA-beads incorporated into living cells
| Project/Area Number |
22770205
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | National Institute of Information and Communications Technology |
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Principal Investigator |
KOBAYASHI Shouhei 独立行政法人情報通信研究機構, 未来ICT研究所・バイオICT研究室, 主任研究員 (40425765)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | DNAビーズ / 膜アセンブリー / Live CLEM / 異物認識 / BAF / Live CLEM法 |
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| Research Abstract |
This research aims to investigate molecular mechanisms of assembly of the nuclear envelope(NE)-like membrane by utilizing DNA-beads incorporated into a living cell. We analyzed the timing of assembly of various cellular components on the DNA-beads using fluorescence microscopy and electron microscopy, and found that barrier-to-autointegration factor(BAF) appeared on the beads at very early timing after endosomal breakage. Removal of BAF with RNAi significantly reduced assembly of a nuclear envelope protein on the beads. Based on these results, we conclude that BAF is a critical factor for DNA-beads dependent NE assembly.
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Report
(3 results)
Research Products
(45 results)
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[Journal Article] Transient Focal Membrane Deformation Induced by Arginine-rich Peptides Leads to Their Direct Penetration into Cells2012
Author(s)
Hirose, H., Takeuchi, T., Osakada, H., Pujals, S., Katayama, S., Nakase, I., Kobayashi, S., Haraguchi, T., Futaki, S
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Journal Title
Molecular Therapy
Volume: (in press)
Issue: 5
Pages: 984-993
DOI
Related Report
Peer Reviewed
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[Presentation] The Nuclear Envelope Structures are Required for Maintenance of the Micronucleus Formed by Loss of Centromere Functions of a Human Artificial Chromosome2011
Author(s)
Haraguchi, T., Koujin, T., Osakada, H., Kojidani, T., Kobayashi, S., Nakano, M., Masumoto, H., Larionov, V. L., Earnshaw, W. C., Hiraoka, Y.
Organizer
Nuclear envelope disease and chromatin organization
Place of Presentation
Robinson College, Cambridge, UK
Year and Date
2011-07-14
Related Report
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[Presentation] Assembly of intracellular membrane around DNA-coated polystyrene beads in living HeLa cells2011
Author(s)
Kobayashi, S., Kojidani, T., Osakada, H., Mori, C., Koujin, T., Hiraoka, Y., Haraguchi, T.
Organizer
International Symposium on the Physicochemical Field for Genetic Activities
Place of Presentation
The Westin Awaji Island, Japan
Year and Date
2011-01-24
Related Report
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[Presentation] The Nuclear Envelope Structures are Required for Maintenance of the Micronucleus Formed by Loss of Centromere Functions of a Human Artificial Chromosome2011
Author(s)
Koujin, T., Osakada, H., Kojidani, T., Kobayashi, S., Nakano, M., Masumoto, H., Larionov, V. L., Earnshaw, W. C., Hiraoka, Y., Haraguchi, T.
Organizer
International Symposium on the Physicochemical Field for Genetic Activities
Place of Presentation
The Westin Awaji Island, Japan
Year and Date
2011-01-24
Related Report
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[Presentation] The nuclear envelope structures are required for maintenance of the micronucleus formed by loss of centromere functions of a human artificial chromosome2010
Author(s)
Haraguchi, T., Koujin, T., Osakada, H., Kojidani, T., Kobayashi, S., Nakano, M., Masumoto, H., Larionov, V., Earnshaw, W. C., Hiraoka, Y.
Organizer
The American Society for Cell Biology Annual Meeting
Place of Presentation
Philadelphia Pennsylvania Convention Center U.S.A
Year and Date
2010-12-13
Related Report
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