Exploring novel proteases involved in the fertilization and developmental mechanisms of ascidian

• Project/Area Number: 22770209
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Nagoya University
• Principal Investigator: YAMADA Lixy 名古屋大学, 理学研究科, 特任助教 (10551020)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: プロテアーゼ / ゲノム / 発生 / 受精 / ホヤ / 進化 / 発生・分化

Research Abstract

The ascidian Ciona intestinalis is belong to the phylum chordate and share many fundamental aspects of the chordate body plan with vertebrates. We have revealed 506 protease genes in Ciona by the comprehensive annotation and phylogenetic analyzes based on genomic information. Utilizing known microarray and EST data, eight protease genes that show testis-specific expressions and the other eight protease genes which show expressions in the specific stage during development were selected. We have revealed detailed expression patterns of these 16 protease genes in embryos and adult tissues by RT-PCR and whole mount in situ hybridization.

Research Products

• [Journal Article] CIPRO 2. 5 : Ciona intestinal is protein database, a unique integrated repository of large-scale omics data, bioinformatic analyses and curate annotation, with user rating and reviewing functionality (2011)
  • Author(s): Endo, T., Ueno, K., Yonezawa, K., Mineta, K., Hotta, K., Satou, Y., Yamada, L. Endo, T., Ueno, K., Yonezawa, K., Mineta, K., Hotta, K., Satou, Y., Yamada, L.
  • Journal Title: Nucleic Acids Res Volume: 39 Pages: 807-814
  • Peer Reviewed
• [Journal Article] Identification and localization of the sperm CRISP family protein CiUrabin involved in gamete interaction in the ascidian Ciona intestinal is (2011)
  • Author(s): Yamaguchi, A., Saito, T., Yamada, L., Taniguchi, H., Harada, Y., and Sawada, H
  • Journal Title: Molecular Reproduction and Development Volume: 78(7) Pages: 488-497
  • Peer Reviewed
• [Journal Article] Identification and localization of the sperm CRISP family protein CiUrabin involved in gamete interaction in the ascidian Ciona intestinalis (2011)
  • Author(s): Akira Yamaguchi
  • Journal Title: Molecular Reproduction and Development Volume: 78(7) Pages: 488-497
  • Peer Reviewed
• [Journal Article] CIPRO 2.5 : Ciona intestinalis protein database, a unique integrated repository of large-scale omics data, bioinformatic analyses and curated annotation, with user rating and reviewing functionality. (2011)
  • Author(s): Toshinori Endo
  • Journal Title: Nucleic Acids Res. Volume: 39 Pages: 807-814
  • Peer Reviewed
• [Presentation] 非モデル生物におけるプロテオミクス解析の実践と可能性 (2012)
  • Author(s): 山田力志
  • Organizer: 研究会「モデル生物・非モデル生物のプロテオミクスが拓く生物学」
  • Place of Presentation: 自然科学研究機構岡崎コンファレンスセンター、岡崎市
  • Year and Date: 2012-05-14
• [Presentation] 非モデル生物におけるプロテオミクス解析の実践と可能性 (2012)
  • Author(s): 山田力志
  • Organizer: 研究会「モデル生物・非モデル生物のプロテオミクスが拓く生物学」
  • Place of Presentation: 自然科学研究機構岡崎コンファレンスセンター、岡崎市(招待講演)(発表確定)
  • Year and Date: 2012-05-14
• [Presentation] カタユウレイボヤにおける精子及び卵膜ラフト画分のプロテオーム解析(Global protein profiling of sperm and egg membrane raft fraction during fertilization in Ciona intestinal is.) (2011)
  • Author(s): 山田力志
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜、横浜
  • Year and Date: 2011-12-14
• [Presentation] カタユウレイボヤにおける精子及び卵膜ラフト画分のプロテオーム解析(Global protein profiling of sperm and egg membrane raft fraction during fertilization in Ciona intestinalis.) (2011)
  • Author(s): 山田力志
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜、横浜
  • Year and Date: 2011-12-14
• [Presentation] マイナー生物におけるプロテオーム解析の試み (2011)
  • Author(s): 山田力志
  • Organizer: シンポジウム
  • Place of Presentation: NAIST(生駒市)
  • Year and Date: 2011-11-24
• [Presentation] マイナー生物におけるプロテオーム解析の試み (2011)
  • Author(s): 山田力志
  • Organizer: シンポジウム「プロテオミクスを生命科学に生かす10の方法」
  • Place of Presentation: NAIST(生駒市)(招待講演)
  • Year and Date: 2011-11-24
• [Presentation] Grobal protein profiling of sperm and egg lipid-raft membrane during fertilization in Ciona intestinal is (2011)
  • Author(s): Lixy Yamada
  • Organizer: 6th International Tunicate Meeting, McGill University
  • Place of Presentation: Montreal, Quebec, Canada
  • Year and Date: 2011-07-07
• [Presentation] Grobal protein profiling of sperm and egg lipid-raft membrane during fertilization in Ciona intestinalis (2011)
  • Author(s): Lixy Yamada
  • Organizer: 6th International Tunicate Meeting
  • Place of Presentation: 6th International Tunicate Meeting
  • Year and Date: 2011-07-07
• [Presentation] 仮想ゲルを用いたLC/MSデータの表示とタンパク質修飾の探索プログラム(Western Blot-like Presentation of Gel-enhanced LC/MS Data and Software-based Detection of Protein Modifications) (2010)
  • Author(s): 吉沢明康
  • Organizer: BMB2010(第33回日本分子生物学会年会、第83回日本生化学会大会合同大会)
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] CIPRO 2. 5 : Ciona intestinal is Protein integrated database with large-scale omics data, bioinformatic analyses and curated annotation, with ability for user rating and comments (2010)
  • Author(s): Toshinori Endo
  • Organizer: Biocuration 2010
  • Place of Presentation: Odaiba, Tokyo, JAPAN
  • Year and Date: 2010-10-14
• [Presentation] CIPRO 2. 5 : Cionaintestinalis Protein Database, a unique integrated repository of large-scale omics data, bioinformatic analyses, and curated annotation, with ability for user rating andcomments (2010)
  • Author(s): Toshinori Endo
  • Organizer: Beyond the Genome
  • Place of Presentation: Harvard Medical School, Boston, Massachusetts, U.S.A.
  • Year and Date: 2010-10-12
• [Presentation] Ciona intestinal is protein database CIPRO shows a variety of proteomes for a single species (2010)
  • Author(s): Toshinori Endo
  • Organizer: the annual CBRC open house workshop(CBRC2010) on Bioinformatics
  • Place of Presentation: Tokyo
  • Year and Date: 2010-07-29
• [Presentation] Ciona intestinalis protein database CIPRO shows a variety of proteomes for a single species (2010)
  • Author(s): Toshinori Endo
  • Organizer: the annual CBRC open house workshop (CBRC2010) on Bioinformatics
  • Place of Presentation: Computational Biology Research Center (Tokyo)
• [Presentation] CIPRO 2.5 : Ciona intestinalis Protein Database, a unique integrated repository of large-scale omics data, bioinformatic analyses, and curated annotation, with ability for user rating and comments (2010)
  • Author(s): Toshinori Endo
  • Organizer: Beyond the Genome
  • Place of Presentation: Harvard Medical School, (Boston, Massachusetts, U.S.A.)
• [Presentation] CIPRO 2.5 : Ciona intestinalis Protein integrated database with large-Beale omics data, bioinformatic analyses and curated annotation, with ability for user rating and comments (2010)
  • Author(s): Toshinori Endo
  • Organizer: Biocuration 2010
  • Place of Presentation: Tokyo International Exchange Center, (Tokyo)
• [Presentation] 仮想ゲルを用いたLC/MSデータの表示とタンパク質修飾の探索プログラム(Western Blot-like Presentation of Gel-enhanced LC/MS Data and Software-based Detection of Protein Modifications) (2010)
  • Author(s): 吉沢明康
  • Organizer: BMB2010(第33回日本分子生物学会年会、第83回日本生化学会大会合同大会)
  • Place of Presentation: 神戸ポートアイランド(神戸)
• [Book] Sperm Cell Research in the 21st Century : Historical Discoveries to New Horizons (2012)
  • Author(s): Hitoshi Sawada
  • Publisher: Adthree Publishing Co.(出版確定)(未定)
• [Book] Sperm Cell Research in the 21st Century : Historical Discoveries to New Horizons (2011)
  • Author(s): Sawada, H., Saito, T., Yamaguchi, A., Harda, Y., and Yamada L.
  • Publisher: Adthree Publishing Co.
• [Remarks] (1)研究開発web CIPRO (Ciona intestinalis protein database)
  • URL: http://cipro.ibio.jp/current/