Analysis of dynamics of histone modifications during embryogenesis of the ascidian Ciona intestinalis

• Project/Area Number: 22770223
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Nagahama Institute of Bio-Science and Technology
• Principal Investigator: WADA Shuichi 長浜バイオ大学, バイオサイエンス学部, 講師 (20378607)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 遺伝子発現調節 / ヒストン修飾 / 発生・分化 / 発現制御 / ヒストンメチル化修飾 / エピジェネティクス / エピジェテクス

Research Abstract

To understand the role of histone modifications in animal embryogenesis, the histone methylation pattern and the expression of genes for histone lysine methyltransferases(HKMTs) and histone lysine demethylases(HKDMs) during ascidian embryogenesis were investigated. It has been shown that histone H3 lysine 4(H3K4) methylation is an active mark for transcription while histone H3 lysine 27(H3K27) methylation marks repressive states of transcription. ChIP-qPCR analysis of the distribution of methylated H3K4 in several selected regions of the genome of Ciona tailbud stage embryos revealed that the methylated H3K4 mark is associated with the transcribed region of active genes. Expression patterns of almost all HKMT and HKDM genes were examined and genes that are expressed during Ciona embryogenesis were identified.

Research Products

• [Presentation] Analysis of histone methylation in the ascidian Ciona intestinalis (2011)
  • Author(s): 森田真希、和田修一
  • Organizer: 6th International Tunicate Meeting
  • Place of Presentation: カナダ・モントリオール・マギル大学
  • Year and Date: 2011-07-06
• [Presentation] Analysis of histone methylation in the ascidian Ciona intestinalis (2011)
  • Author(s): 森田真希
  • Organizer: 6th International Tunicate Meeting
  • Place of Presentation: マギル大学(カナダ・モントリオール市)
  • Year and Date: 2011-07-06
• [Presentation] カタユウレイボヤにおけるヒストンメチル化酵素とヒストン脱メチル化酵素の網羅的解析 (2010)
  • Author(s): 森田真希、和田修一
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] カタユウレイボヤにおけるヒストンメチル化酵素とヒストン脱メチル化酵素の網羅的解析 (2010)
  • Author(s): 森田真希
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学学会合同大会
  • Place of Presentation: 神戸ポトアイランド
  • Year and Date: 2010-12-07