Analysis of WYMV resistance gene using NBRP KOMUGI database

Research Project

Project/Area Number	22780008
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Breeding science
Research Institution	Hokkaido Research Organization
Principal Investigator	SUZUKI Takako 地方独立行政法人北海道立総合研究機構, 農業研究本部中央農業試験場作物開発部, 主査 (00462375)
Project Period (FY)	2010 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000) Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords	植物育種 / 遺伝 / 抵抗性 / 耐性 / コムギ / DNAマーカー / 縞萎縮病 / 育種学 / 遺伝子 / ゲノム
Research Abstract	In this study 151 recombinant inbred lines (RILs) (F9) from a cross between ‘Madsen’ (resistant to WYMV) and ‘Hokushin’ (susceptible to WYMV) were tested in the field trials at a WYMV nursery. 151 RILs were detected WYMV by ELISA and genotyped by NBRP recommended markers. Two major QTLs associated with WYMV resistance were detected on chromosome 2DL between and 3BS. In addition DNA marker linked to resistance gene on 2DL was developed