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Live-imaging analysis of mitochondrial movement in rice cells during rice. Magnaporthe oryzae interactions

Research Project

Project/Area Number 22780044
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Plant pathology
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

MOCHIZUKI Susumu  独立行政法人農業生物資源研究所, 遺伝子組換え研究センター耐病性作物研究開発ユニット, 特別研究員 (40567020)

Research Collaborator NISHIZAWA Yoko  農業生物資源研究所, 耐病性作物研究開発ユニット, 上級研究員 (40355756)
KUBO Yasuyuki  京都府立大学, 生命環境科学研究科, 教授 (80183797)
YAMANE Hisakazu  帝京大学, 理工学部・バイオサイエンス学科, 教授 (80090520)
OKADA Kazunori  東京大学, 生物生産工学研究センター, 助教 (20312241)
Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsミトコンドリア / イネ / いもち病菌 / 病害抵抗性 / ライブイメージング / 過敏感細胞死
Research Abstract

I improved the live and real-time imaging method using trimmed and sliced leaf-sheath to analyze the structural changes and the intracellular reactions both in a single cell of rice and blast fungus during the early infection process. Using this method, two types of alteration of mitochondrial distribution were observed. Observation using some mutants of the rice blast fungus revealed that both of types of the alteration were closely related to resistant reaction of rice cells to the blast. Moreover, I applied the imaging method to observe some cellular reactions during the infection and wounding.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (20 results)

All 2012 2011 2010

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (14 results) Book (2 results)

  • [Journal Article] Stress-induced expression of the transcription factor RERJ1 is tightly regulated in response to jasmonic acid accumulation in rice2012

    • Author(s)
      Koji Miyamoto, Takafumi Shimizu, Susumu Mochizuki, Yoko Nishizawa, Eiichi Minami, Hideaki Nojiri, Hisakazu Yamane and Kazunori Okada
    • Journal Title

      Protoplasma

      Volume: (in press) Issue: 1 Pages: 241-249

    • DOI

      10.1007/s00709-012-0400-z

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of a bacterial chitosanase in rice plants improves disease resistance to the rice blast fungus Magnaporthe oryzae2012

    • Author(s)
      Kouzai Y, Mochizuki S, Saito A, Ando A, Minami E, Nishizawa Y
    • Journal Title

      Plant Cell Rep

      Volume: 31 Issue: 4 Pages: 629-636

    • DOI

      10.1007/s00299-011-1179-7

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Localization of probe-accessible chitin and characterization of genes encoding chitin-binding domains during rice-Magnaporthe oryzae interactions2011

    • Author(s)
      Susumu Mochizuki, Ken-ichiro Saitoh, Eiichi Minami and Yoko Nishizawa
    • Journal Title

      Journal of general plant pathology

      Volume: 77 Issue: 3 Pages: 163-173

    • DOI

      10.1007/s10327-011-0310-5

    • NAID

      10029721073

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Localization of probe-accessible chitin and characterization of genes encoding chitin-binding domains during rice-Magnaporthe oryzae interactions2011

    • Author(s)
      Susumu Mochizuki, Ken-ichiro Saitoh, Eiichi Minami, Yoko Nishizawa
    • Journal Title

      Journal of General Plant Pathology

      Volume: 77(3) Pages: 163-173

    • NAID

      10029721073

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] イネいもち病菌感染初期過程におけるミトコンドリア凝集反応の観察2012

    • Author(s)
      望月進,齋藤憲一郎,久保康之,南栄一,西澤洋子
    • Organizer
      日本植物病理学会
    • Place of Presentation
      福岡
    • Related Report
      2011 Final Research Report
  • [Presentation] 細菌キトサナーゼを発現するイネの作製とそのいもち病抵抗性の解析2012

    • Author(s)
      香西雄介,望月進,齋藤明広,安藤昭一,南栄一,西澤洋子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都
    • Related Report
      2011 Final Research Report
  • [Presentation] 高濃度窒素条件下におけるイネユビキチンリガーゼEL5の機能解析2012

    • Author(s)
      望月進,中島恵美,軸丸祐介,神谷勇治,南栄一,西澤洋子
    • Organizer
      日本植物生理学会
    • Place of Presentation
      京都
    • Related Report
      2011 Final Research Report
  • [Presentation] 高濃度窒素条件下におけるイネユビキチンリガーゼEL5の機能解析2012

    • Author(s)
      望月進
    • Organizer
      日本植物生理学会
    • Place of Presentation
      京都産業大学(京都市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 細菌キトサナーゼを発現するイネの作製とそのいもち病抵抗性の解析2012

    • Author(s)
      香西雄介
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都女子大学(京都市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] イネいもち病菌感染初期過程におけるミトコンドリア凝集反応の観察2012

    • Author(s)
      望月進
    • Organizer
      日本植物病理学会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] イネ剥離葉鞘裏面細胞を用いたいもち病菌感染初期における細胞内構造変化のリアルタイムイメージング法の構築2011

    • Author(s)
      望月進、南栄一、西澤洋子
    • Organizer
      日本植物病理学会
    • Place of Presentation
      (書誌上)
    • Year and Date
      2011-03-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] キトサン分解能を付与した形質転換イネの作出とそのいもち病抵抗性の解析2011

    • Author(s)
      香西雄介,望月進,齋藤明広,安藤昭一,南栄一,西澤洋子
    • Organizer
      日本植物病理学会関東部会
    • Place of Presentation
      つくば
    • Related Report
      2011 Final Research Report
  • [Presentation] Biological Function of the JA-inductive bHLH Transcription Factor RERJ1 in Rice Terpenoids Synthesis2011

    • Author(s)
      Kazunori Okada, Koji Miyamoto, Takafumi Shimizu, Tatsuya Kitajima, Koichi Kawamoto, Tetsuya Chujo, Rika Ozawa, Junji Takabayashi, Susumu Mochizuki, Yoko Nishizawa, Akio Miyao, Hirohiko Hirochika, Hideaki Nojiri, Hisakazu Yamane
    • Organizer
      10th International Meeting : Biosynthesis and Functional Isoprenoids in Plants
    • Place of Presentation
      Microorganisms and Parasites
    • Related Report
      2011 Final Research Report
  • [Presentation] イネ剥離葉鞘裏面細胞を用いたいもち病菌感染初期における細胞内構造変化のリアルタイムイメージング法の構築2011

    • Author(s)
      望月進,南栄一,西澤洋子
    • Organizer
      日本植物病理学会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] Biological Function of the JA-inductive bHLH Transcription Factor RERJ1 in Rice Terpenoids Synthesis2011

    • Author(s)
      Kazunori Okada
    • Organizer
      10th International Meeting : Biosynthesis and Functional Isoprenoids in Plants, Microorganisms and Parasites
    • Place of Presentation
      Kalmar, Sweden
    • Related Report
      2011 Annual Research Report
  • [Presentation] キトサン分解能を付与した形質転換イネの作出とそのいもち病抵抗性の解析2011

    • Author(s)
      香西雄介
    • Organizer
      日本植物病理学会関東部会
    • Place of Presentation
      文部科学省研究交流センター(茨城県つくば市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] いもち病菌感染初期におけるイネの細胞内構造変化の可視化2010

    • Author(s)
      望月進、南栄一、西澤洋子
    • Organizer
      日本植物病理学会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Year and Date
      2010-04-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] いもち病菌感染初期におけるイネの細胞内構造変化の可視化2010

    • Author(s)
      望月進,南栄一,西澤洋子
    • Organizer
      日本植物病理学会
    • Place of Presentation
      京都
    • Related Report
      2011 Final Research Report
  • [Book] Defense mechanisms mediated by chitin in rice-blast interactions. Genome-Enabled Analysis of Plant-Pathogen Interactions2011

    • Author(s)
      Yoko Nishizawa, Susumu Mochizuki, Ken-ichiro Saitoh, Kyutaro Kishimoto, Yusuke Kouzai, Eiichi Minami
    • Publisher
      The American Phytopathological Society Press
    • Related Report
      2011 Final Research Report
  • [Book] Genome-Enabled Analysis of Plant-Pathogen Interactions2011

    • Author(s)
      西澤洋子
    • Publisher
      American Phytopathological Society Press
    • Related Report
      2011 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

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