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Mechanism of substrate recognition by bacterial lipoprotein transport equipment

Research Project

Project/Area Number 22780062
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionKyoto University (2011)
The University of Tokyo (2010)

Principal Investigator

NARITA Shin-ichiro  京都大学, ウイルス研究所, 特定助教 (30338751)

Research Collaborator TOKUDA Hajime  盛岡大学, 栄養科学部, 教授 (40125943)
Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords細菌細胞表層 / ABCトランスポーター / 細菌 / 蛋白質 / リポ蛋白質 / トランスポーター / 細胞表層
Research Abstract

Bacterial lipoproteins represent a subset of membrane-associated proteins that are covalently modified with lipids at the N-terminal cysteine. The final step of lipoprotein modification, N-acylation of apolipoproteins, is mediated by Lnt. lnt null strains could be constructed when LolCDE was overproduced in strains lacking either the major outer membrane lipoprotein Lpp or transpeptidases that cross-link Lpp with peptidoglycan. We revealed that affinity of LolCDE for apolipoprotein is very low, and therefore overexpression of LolCDE is required for its release and sorting to the outer membrane.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (26 results)

All 2012 2011 2010 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (10 results) Book (2 results) Remarks (2 results)

  • [Journal Article] ABC transporters involved in the biogenesis of the outer membrane in gram-negative bacteria2011

    • Author(s)
      Shin-ichiro Narita
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 75 Pages: 1044-1054

    • NAID

      10029327715

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Over expression of LolCDE allows the deletion of the Escherichia coli gene encoding apolipoprotein N-acyltransferase2011

    • Author(s)
      Shin-ichiro Narita and Hajime Tokuda
    • Journal Title

      J. Bacteriol

      Volume: 193 Pages: 4832-4830

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] ABC Transporters Involved in the Biogenesis of the Outer Membrane in Gram-Negative Bacteria2011

    • Author(s)
      Shin-ichiro Narita
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 75 Issue: 6 Pages: 1044-1054

    • DOI

      10.1271/bbb.110115

    • NAID

      10029327715

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of LolCDE allows deletion of the Escherichia coli gene encoding apolipoprotein N-acyltransferase.2011

    • Author(s)
      Narita, S., and Tokuda, H.
    • Journal Title

      J. Bacteriol.

      Volume: 193 Issue: 18 Pages: 4832-4840

    • DOI

      10.1128/jb.05013-11

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ABC transporters involved in the biogenesis of the outer membrane in gram-negative bacteria.2011

    • Author(s)
      Shin-ichiro Narita
    • Journal Title

      Biosci.Biotechnol.Biochem.

      Volume: (印刷中)

    • NAID

      10029327715

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of an inducible system to engineer unmarked conditional mutants of essential genes of Pseudomonas aeruginosa2010

    • Author(s)
      Yuji Morita, Shin-ichiro Narita, Junko Tomida, Hajime Tokuda and Yoshiaki Kawamura
    • Journal Title

      J. Microbiol. Methods

      Volume: 82 Pages: 205-213

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A periplasmic LolA derivative with a lethal disulfide bond activates the Cpx stress response system2010

    • Author(s)
      Kazuyuki Tao, Shoji Watanabe, Shin-ichiro Narita and Hajime Tokuda
    • Journal Title

      J. Bacteriol

      Volume: 192 Pages: 5657-5662

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Novel mutations of the LolCDE complex causing outer membrane localization of lipoproteins despite their inner membrane retention signals2010

    • Author(s)
      Chihiro Sakamoto, Rika Satou, Hajime Tokuda and Shin-ichiro Narita
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 401 Pages: 586-591

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A periplasmic LolA derivative with a lethal disulfide bond activates the Cpx stress response system.2010

    • Author(s)
      Kazuyuki Tao, Shoji Watanabe, Shin-ichiro Narita, Hajime Tokuda
    • Journal Title

      J.Bacteriol.

      Volume: 192 Pages: 5657-5662

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel mutations of the LolCDE complex causing outer membrane localization of lipoproteins despite their inner membrane retention signals.2010

    • Author(s)
      Chihiro Sakamoto, Rika Satou, Hajime Tokuda, Shin-ichiro Narita
    • Journal Title

      Biochem.Biophys.Res.Commun.

      Volume: 401 Pages: 586-591

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sorting of bacterial lipoproteins to the outer membrane by the Lol system.2010

    • Author(s)
      Shin-ichiro Narita, Hajime Tokuda
    • Journal Title

      Methods in Molecular Biology

      Volume: 619 Pages: 117-129

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biogenesis and membrane targeting of lipoproteins.2010

    • Author(s)
      Shin-ichiro Narita, Hajime Tokuda
    • Journal Title

      EcoSal-Escherichia coli and Salmonella : Cellular and Molecular Biology.

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 大腸菌細胞表層の品質管理に関わるプロテアーゼホモログYfgCの機能解析2012

    • Author(s)
      成田新一郎、秋山芳展
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎
    • Related Report
      2011 Final Research Report
  • [Presentation] 大腸菌細胞表層の品質管理に関わるプロテアーゼホモログYfgCの機能解析2012

    • Author(s)
      成田新一郎
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール
    • Related Report
      2011 Annual Research Report
  • [Presentation] 大腸菌リポ蛋白質の外膜局在化に関わるABCトランスポーターの解析2011

    • Author(s)
      成田新一郎
    • Organizer
      2010年度 国立遺伝学研究所研究会
    • Place of Presentation
      国立遺伝学研究所(招待講演)
    • Year and Date
      2011-03-31
    • Related Report
      2010 Annual Research Report
  • [Presentation] 大腸菌表層タンパク質の品質管理に関わる新規プロテアーゼホモログの解析2011

    • Author(s)
      成田新一郎、秋山芳展
    • Organizer
      第8回21世紀大腸菌研究会
    • Place of Presentation
      南木曽
    • Related Report
      2011 Final Research Report
  • [Presentation] 単細胞システムの細胞構築と増殖制御の研究,大腸菌リポ蛋白質の外膜局在化に関わるABCトランスポーターの解析2011

    • Author(s)
      成田新一郎
    • Organizer
      2010年度国立遺伝学研究所研究会
    • Place of Presentation
      国立遺伝学研究所
    • Related Report
      2011 Final Research Report
  • [Presentation] グラム陰性細菌の細胞表層形成に関与するABCトランスポーターの研究2011

    • Author(s)
      成田新一郎
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] グラム陰性細菌の細胞表層形成に関与するABCトランスポーターの研究2010

    • Author(s)
      成田新一郎
    • Organizer
      日本農芸化学会関東支部2010年度第1回支部例会
    • Place of Presentation
      筑波大学
    • Year and Date
      2010-07-17
    • Related Report
      2011 Final Research Report
  • [Presentation] グラム陰性細菌の細胞表層形成に関与するABCトランスポーターの研究2010

    • Author(s)
      成田新一郎
    • Organizer
      日本農芸化学会関東支部 2010年度 第1回支部例会
    • Place of Presentation
      筑波大学(招待講演)
    • Year and Date
      2010-07-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] 細菌リポタンパク質の外膜局在化を司るABCトランスポーターLolCDEの機能2010

    • Author(s)
      成田新一郎、徳田元
    • Organizer
      第5回トランスポーター研究会年会
    • Place of Presentation
      東京医科大学病院(招待講演)
    • Year and Date
      2010-07-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] 細菌リポタンパク質の外膜局在化を司るABCトランスポーターLolCDEの機能2010

    • Author(s)
      成田新一郎、徳田元
    • Organizer
      第5回トランスポーター研究会年会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Book] Sorting of bacterial lipoproteins to the outer membrane by the Lol system2010

    • Author(s)
      Shin-ichiro Narita and Hajime Tokuda
    • Publisher
      Humana Press
    • Related Report
      2011 Final Research Report
  • [Book] Biogenesis and membrane targeting of lipoproteins2010

    • Author(s)
      Shin-ichiro Narita and Hajime Tokuda
    • Publisher
      ASM Press
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.virus.kyoto-u.ac.jp/Lab/akiyama/index.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.virus.kyoto-u.ac.jp/Lab/akiyama/index.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

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