Live cell imaging of prokaryotic magnetic organelle to analyze mechanisms of organelle formation and function
Project/Area Number |
22780063
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Applied microbiology
|
Research Institution | Kanazawa University |
Principal Investigator |
TAOKA Azuma 金沢大学, 自然システム学系, 助教 (20401888)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 原核細胞オルガネラ / 生細胞イメージング / 磁気微粒子 / バイオミネラリゼーション / 細菌 / 磁性 / 蛋白質 / イメージング / オルガネラ / マグネトソーム / 微生物学 / 原核細胞 |
Research Abstract |
Magnetotactic bacteria possess nano-sized prokaryotic magnetic organelles, magnetosomes. We developed live cell imaging technique by using fluorescence proteins to analyze cellular dynamics of the magnetosomes. By fusions of fluorescence proteins with magnetosomal proteins, we labeled magnetosome membrane and magnetosomal cytoskeleton in Magnetospirillum magneticum AMB-1. We successfully observed segregation of the magnetosomes to daughter cells during cell division.
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Report
(4 results)
Research Products
(36 results)