Elucidation of yeast stress mechanism with dynamic metabolic profiling and development of recombinant yeast strains resistant to stress

• Project/Area Number: 22780092
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry
• Research Institution: Kobe University
• Principal Investigator: HASUNUMA Tomohisa 神戸大学, 自然科学系先端融合研究環重点研究部, 講師 (20529606)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 代謝工学 / 酵母 / 代謝解析 / バイオエタノール / 発酵阻害物耐性 / メタボローム / 発酵阻害物質耐性

Research Abstract

For the efficient ethanol production from lignocellulosic biomass, recombinant Saccharomyces cerevisiae strains that have improved tolerance to fermentation inhibitors were rationally developed based on the newly developed system biological analysis.

Research Products

• [Journal Article] Deletion of the PHO13 gene in Sacc haromyces cerevisiae improves ethanol production from lignocellulosic hydrolys ate in the presence of acetic and formic acids, and furfural (2012)
  • Author(s): Fujitomi K, Sanda T, Hasunuma T, Kondo A
  • Journal Title: Bioresource Tech nology Volume: Vol.111 Pages: 161-166
  • DOI: 10.1016/j.biortech.2012.01.161
  • Peer Reviewed
• [Journal Article] Improvements in ethanol production from xylose by mating recombinant xylose-fermenting Saccharomyces cerevisiae strains (2012)
  • Author(s): Kato H, Suyama H, Yamada R, Hasunuma T, Kondo A
  • Journal Title: Applied Microbiology and Biotechnology Volume: Vol.94 Pages: 1585-1592
  • Peer Reviewed
• [Journal Article] Display of cellulases on the cell surface of Saccharomyces cerevisiae affords high yield ethanol production from high-solid lignocellulosic biomass. (2012)
  • Author(s): Matano Y, Hasunuma T, Kondo A
  • Journal Title: Bioresource Technology Volume: Vol.108 Pages: 128-133
  • DOI: 10.1016/j.biortech.2011.12.144
  • Peer Reviewed
• [Journal Article] Development of yeast cell factories for consolidated bioprocessing of lignocellulose to bioethanol through cell surface engineering (2012)
  • Author(s): Hasunuma T, Kondo A
  • Journal Title: Biotechnology Advances Volume: (in press) Issue: 6 Pages: 1207-1218
  • DOI: 10.1016/j.biotechadv.2011.10.011
  • Peer Reviewed
• [Journal Article] Widely targeted metabolic profiling analysis of yeast central metabolites (2012)
  • Author(s): Kato H, Izumi Y., Hasunuma T, Matsuda F, Kondo A
  • Journal Title: Journal of Bioscience and Bioengineering Volume: Vol.113 Issue: 5 Pages: 665-673
  • DOI: 10.1016/j.jbiosc.2011.12.013
  • NAID: 110009456911
  • Peer Reviewed
• [Journal Article] Direct ethanol production from rice straw hydrolysate by use of an engineered yeast strain codisplaying three types of hemicellulolytic enzymes on the surface of xylose-utilizing Saccharomyces cerevisiae cells (2012)
  • Author(s): Sakamoto T, Hasunuma T, Hori Y, Yamada R, Kondo A
  • Journal Title: Journal o f Biotechnology Volume: Vol.158 Issue: 4 Pages: 203-210
  • DOI: 10.1016/j.jbiotec.2011.06.025
  • Peer Reviewed
• [Journal Article] Repeated-batch fermentation of lignocellulosic hydrolysate to ethanol using a hybrid Saccharomyces cerevisiae strain metabolically engineered for tolerance to acetic and formic acids. (2011)
  • Author(s): Sanda T, Hasunuma T, Matsuda F, Kondo A
  • Journal Title: Bioresource Technology Volume: Vol.102 Issue: 17 Pages: 7917-7924
  • DOI: 10.1016/j.biortech.2011.06.028
  • Peer Reviewed
• [Journal Article] Metabolic path way engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae (2011)
  • Author(s): Hasunuma T, Sanda T, Yamada R, Yoshimura K, Ishii J, Kondo A
  • Journal Title: Microb ial Cell Factories Volume: Vol.10 Issue: 1 Pages: 2-2
  • DOI: 10.1186/1475-2859-10-2
  • Peer Reviewed
• [Journal Article] Efficient ferm entation of xylose to ethanol at high formic acid concentration by metabolically engineered Saccharomyces cerevisiae (2011)
  • Author(s): Hasunuma T, Sung K, Sanda T, Yoshimura K, Matsuda F, Kondo A
  • Journal Title: Applied Microbiology and Biotechnology Volume: Vol.9 Issue: 3 Pages: 997-1004
  • DOI: 10.1007/s00253-011-3085-x
  • Peer Reviewed
• [Journal Article] Co-fermentation of cellobiose and xylose using beta-glucosidase displaying diploid industrial yeast strain OC-2 (2011)
  • Author(s): Saito S, Hasunuma T, Tanaka T, Kondo A
  • Journal Title: Applied Microbiology and Biotechnology Volume: Vol.87 Issue: 5 Pages: 1975-1982
  • DOI: 10.1007/s00253-010-2714-0
  • Peer Reviewed
• [Journal Article] Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae (2011)
  • Author(s): Hasunuma T, Sanda T, Yamada R, Yoshimura K, Ishii J, Kondo A
  • Journal Title: Microbial Cell Factories Volume: 10(掲載確定)
  • Peer Reviewed
• [Journal Article] Efficient fermentation of xylose to ethanol at high formic acid concentration by metabolically engineered Saccharomyces cerevisiae (2011)
  • Author(s): Hasunuma T, Sung K, Sanda T, Yoshimura K, Matsuda F, Kondo A
  • Journal Title: Applied Microbiology and Biotechnology Volume: (In press)
  • Peer Reviewed
• [Presentation] Microbial engineering for production of biofuels and biorefinery products (2012)
  • Author(s): Hasunuma T
  • Organizer: 14^<th> A-IMBN Annual Conference : Life Science and Frontier of Biorefinery Technology
  • Place of Presentation: Thailand Science Park(Thailand)
  • Year and Date: 2012-03-01
• [Presentation] Microbial engineering for production of biofuels and biorefinery products (2012)
  • Author(s): Hasunuma T
  • Organizer: 14^<th> A-IMBN Annual Conference : Life Science and Frontier of Biorefinery Technology
  • Place of Presentation: Thailand Science Park (Thailand)
  • Year and Date: 2012-03-01
• [Presentation] CE-TOFMSを用いたバイオエタノール生産酵母の代謝プロファイリング (2011)
  • Author(s): 蓮沼誠久
  • Organizer: 第31回キャピラリー電気泳動シンポジウム
  • Place of Presentation: 慶應義塾大(山形)
  • Year and Date: 2011-11-10
• [Presentation] CE-TOFMSを用いたバイオエタノール生産酵母の代謝プロファイリング (2011)
  • Author(s): 蓮沼誠久
  • Organizer: 第31回キャピラリー電気泳動シンポジウム
  • Place of Presentation: 鶴岡メタボロームキャンパ(山形県)
  • Year and Date: 2011-11-10
• [Presentation] 統合バイオプロセスによるバイオエタノール生産技術の開発 (2011)
  • Author(s): 蓮沼誠久
  • Organizer: 第5回化学工学イノベーション研究会
  • Place of Presentation: 神戸大学(神戸)
  • Year and Date: 2011-10-21
• [Presentation] 統合バイオプロセスによるバイオエタノール生産技術の開発 (2011)
  • Author(s): 蓮沼誠久
  • Organizer: 第5回化学工学イノベーション研究会
  • Place of Presentation: 神戸大学(兵庫県)
  • Year and Date: 2011-10-21
• [Presentation] 発酵阻害物耐性遺伝子を導入したキシロース資化性二倍体酵母によるリグノセルロース水熱分解液の繰返し発酵 (2011)
  • Author(s): 蓮沼誠久, 近藤昭彦
  • Organizer: 第63回日本生物工学会大会
  • Place of Presentation: 東京農工大(東京)
  • Year and Date: 2011-09-27
• [Presentation] 発酵阻害物耐性遺伝子を導入したキシロース資化性二倍体酵母によるリグノセルロース水熱分解液の繰返し発酵 (2011)
  • Author(s): 蓮沼誠久, 近藤昭彦
  • Organizer: 第63回日本生物工学会大会
  • Place of Presentation: 東京農工大(東京都)
  • Year and Date: 2011-09-27
• [Presentation] シンセティックバイオエンジニアリングによるバイオエタノール生産酵母の育種 (2011)
  • Author(s): 蓮沼誠久
  • Organizer: 日本生物工学会関西支部第99回醗酵学懇話会
  • Place of Presentation: キリンビール神戸工場(神戸)
  • Year and Date: 2011-08-04
• [Presentation] シンセティックバイオエンジニアリングによるバイオエタノール生産酵母の育種 (2011)
  • Author(s): 蓮沼誠久
  • Organizer: 日本生物工学会関西支部第99回醗酵学懇話会
  • Place of Presentation: キリンビール神戸工場(兵庫県)
  • Year and Date: 2011-08-04
• [Presentation] Repeated batch-fermentation of rice straw hydrolysate to ethanol using metabolically engineered Saccharomyces cerevisiae strains (2011)
  • Author(s): Hasunuma T, Kondo A
  • Organizer: SIM Annual Meeting and Exhibition
  • Place of Presentation: Sheraton New Orleans (New Orleans)
  • Year and Date: 2011-07-25
• [Presentation] Repeated batch-fermentation of rice straw hydrolysate to ethanol using metabolically engineered Saccharomyces cerevisiae strains (2011)
  • Author(s): Hasunuma T, Kondo A
  • Organizer: SIM Annual Meeting and Exhibition
  • Place of Presentation: Sheraton New Orleans(New Orleans)
  • Year and Date: 2011-07-02
• [Presentation] Cellulosic ethanol production in Saccharomyces cerevisiae through metabolic pathway engineering based on matabolomics (2011)
  • Author(s): Hasunuma T, Matsuda F, Kondo A
  • Organizer: Asian Congress on Biotechnology 2011
  • Place of Presentation: Shanghai Everbright Convention & Exhibition(Shanghai)
  • Year and Date: 2011-05-12
• [Presentation] Cellulosic ethanol production in Saccharomyces cerevisiae through metabolic pathway engineering based on matabolomics (2011)
  • Author(s): Hasunuma T, Matsuda F, Kondo A
  • Organizer: Asian Congress on Biotechnology 2011
  • Place of Presentation: Shanghai Everbright Conventi on & Exhibition (Shanghai)
  • Year and Date: 2011-05-12
• [Presentation] Ethanol production from lignocellulosic materials through metabolic pathway engineering based on metabolomics (2010)
  • Author(s): Hasunuma T
  • Organizer: 6^<th> iBioK Workshop
  • Place of Presentation: 神戸大学(兵庫県)
  • Year and Date: 2010-12-16
• [Presentation] Production of bio-ethanol from lignocellulosic materials through the engineering of yeast cell surface and metabolic pathway (2010)
  • Author(s): Hasunuma T
  • Organizer: 4^<th> Japan-Korea Bio mass Symposium
  • Place of Presentation: Gekkeikan Conference Hall(京都府)
  • Year and Date: 2010-11-05
• [Presentation] Production of bio-ethanol from lignocellulosic materials through the engineering of yeast cell surface and metabolic pathway (2010)
  • Author(s): Hasunuma T
  • Organizer: 4^<th> Japan-Korea Biomass Symposium
  • Place of Presentation: Gekkeikan Conference Hall(京都府)(招待講演)
  • Year and Date: 2010-11-05
• [Presentation] メタボロームを利用した新規バイオエタノール生産酵母の育種 (2010)
  • Author(s): 蓮沼誠久
  • Organizer: 第5回メタボロームシンポジウム
  • Place of Presentation: グランドエル・サン(山形県)
• [Presentation] Metabolomic approach to identify molecules that are important for acetic acid tolerance in a recombinant xylose-fermenting yeast strain (2010)
  • Author(s): Hasunuma T
  • Organizer: Engineering Conferences International, Metabolic Engineering VIII
  • Place of Presentation: ICC(Jeju Island, Korea)
• [Presentation] Metabolomic approach to identify molecules that are important for acetic acid tolerance in a recombinant xylose-fermenting yeast strain (2010)
  • Author(s): Hasunuma T
  • Organizer: Engineering Conferences International, Metabolic Engineering VIII
  • Place of Presentation: ICC (Jeju Island, Korea)(招待講演)
• [Presentation] メタボロームを利用した新規バイオエタノール生産酵母の育種 (2010)
  • Author(s): 蓮沼誠久
  • Organizer: 第5回メタボロームシンポジウム
  • Place of Presentation: グランドエル・サン(山形県)(招待講演)
• [Book] バイオマス分解酵素研究の最前線,序章2,バイオマス分解酵素研究の新たな展開 (2012)
  • Author(s): 蓮沼誠久, 近藤昭彦
  • Total Pages: 4
  • Publisher: シーエムシー出版
• [Book] バイオマス分解酵素研究の最前線,第28章,セルラーゼ細胞表層提示酵母を用いたバイオマス変換 (2012)
  • Author(s): 山田亮祐, 蓮沼誠久, 近藤昭彦
  • Publisher: シーエムシー出版
• [Book] 微生物機能学,第11章,微生物を利用して有限な化石資源から脱却する (2012)
  • Author(s): 蓮沼誠久, 近藤昭彦
  • Publisher: 三共出版
• [Book] 合成生物工学の隆起-有用物質の新たな生産法構築をめざして-のうち「合成生物工学によるバイオ燃料生産のための微生物細胞工場の創製」 (2012)
  • Author(s): 蓮沼誠久, 近藤昭彦
  • Publisher: シーエムシー出版
• [Book] 合成生物工学の隆起-有用物質の新たな生産法構築をめざして-のうち「合成生物工学によるバイオ燃料生産のための微生物細胞工場の創製」 (2012)
  • Author(s): 蓮沼誠久, 近藤昭彦
  • Publisher: シーエムシー出版(印刷中)
• [Patent(Industrial Property Rights)] エタノールの新規生産方法 (2011)
  • Inventor(s): 近藤昭彦,蓮沼誠久
  • Industrial Property Rights Holder: 神戸大学
  • Industrial Property Number: 2011-146931
  • Filing Date: 2011-07-01
• [Patent(Industrial Property Rights)] エタノールの新規生産方法 (2011)
  • Inventor(s): 近藤昭彦, 蓮沼誠久
  • Industrial Property Rights Holder: 神戸大学
  • Industrial Property Number: 2011-146931
  • Filing Date: 2011-07-01