The electrochemical detection method of the carbohydrate-protein interaction for carbohydrate microarray

• Project/Area Number: 22780107
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: KOMBA Shiro 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所食品バイオテクノロジー研究領域, 主任研究員 (20332273)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
• Keywords: 分子認識 / 糖鎖 / 糖鎖チップ / 電気科学計測

Research Abstract

I have synthesized various sugar linkers and these were examined for recognition of lectins by the surface plasmon resonance method and an electrical signal. As a results of the various examinations, the sugar linkers that consist of 11-mercaptoundecanoic acid, 4,7,10-trioxa-1,13-tridecandiamine, and lactose or maltose for a terminal beta-galactose or alpha-glucose respectively were the best structure for electrical measurement (SWV measurement). I have established the technique that detect interaction between sugar and its recognizing protein (lectin etc.) by electrical measurement.

Research Products

• [Journal Article] Synthesis of glycopyranosyl-(1→2)-N-acetylneuraminic acid nonreducing disaccharides and their evaluation as neuraminidase substrates (2014)
  • Author(s): Shiro Komba, Sachiko Machida
  • Journal Title: Journal of Carbohydrate Chemistry Volume: 33 Issue: 3 Pages: 117-136
  • DOI: 10.1080/07328303.2014.892115
  • Peer Reviewed
• [Journal Article] Lectin-like oxidized LDL receptor-1 is palmitoylated and internalized ligands via caveolae/raft-dependent endocytosis (2013)
  • Author(s): Kumano-Kuramochi Miyuki, Xie Qiuhong, Kajiwara Shoko, Komba Shiro, Minowa Takashi, Machida Sachiko
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 434 Pages: 594-599
  • Peer Reviewed
• [Journal Article] 生物機能の解明と応用のための糖鎖自動合成技術および糖鎖ライブラリー合成技術の開発 (2013)
  • Author(s): 今場司朗
  • Journal Title: 応用糖質科学 Volume: 3 Pages: 50-55
• [Journal Article] Identification of 4-hydroxy-2-nonenal-histidine adducts that serve as ligands for human lectin-like oxidized LDL receptor-1 (2012)
  • Author(s): Kumano-Kuramochi, M., Shimozu, Y., Wakita, C., Ohnishi-Kameyama, M., Shibata, T., Matsunaga, S., Takano-Ishikawa, Y., Watanabe, J., Goto, M., Xie, Q., Komba, S., Uchida, K., and Machida, S.
  • Journal Title: Biochem. J Volume: 442 Issue: 1 Pages: 171-180
  • DOI: 10.1042/bj20111029
  • Peer Reviewed
• [Journal Article] Synthesis of Novel Thioglycoside Analogs as the Substrates and/or the Inhibitors of Cellobiohydrolases (2011)
  • Author(s): 寺内毅、小山善行、町田幸子、春見隆文、今場司朗
  • Journal Title: Journal of Applied Glycoscience Volume: 59 Issue: 1 Pages: 11-19
  • DOI: 10.5458/jag.jag.JAG-2011_012
  • NAID: 10030138276
  • ISSN: 1344-7882, 1880-7291
  • Peer Reviewed
• [Journal Article] A region- and stereo-selective parallel synthesis of five types of trigalactoses on a solid support as a model of a combinatorial oligosaccharide library (2011)
  • Author(s): 今場司朗
  • Journal Title: Journal of Applied Glycoscience Volume: 58 Pages: 1-12
  • Peer Reviewed
• [Journal Article] A Regio- and Stereo-selective Parallel Synthesis of Five Types of Trigalactoses on a Solid Support as a Model of a Combinatorial Oligosaccharide Library (2010)
  • Author(s): S.Komba, T.Terauchi, S.Machida
  • Journal Title: Journal of Applied Glycoscience Volume: 58 Issue: 1 Pages: 1-12
  • DOI: 10.5458/jag.jag.JAG-2010_006
  • NAID: 130004480981
  • ISSN: 1344-7882, 1880-7291
  • Peer Reviewed
• [Presentation] 糖鎖チップの開発 (2013)
  • Author(s): 今場司朗
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 兵庫
  • Year and Date: 2013-12-04
• [Presentation] 糖鎖自動合成も可能にする全自動固相ライブラリー合成機の開発 (2013)
  • Author(s): 今場司朗
  • Organizer: イノベーション・ジャパン2013
  • Place of Presentation: 東京国際展示場（東京都）
• [Presentation] Synthesis of novel thioglycoside analogs as the substrates and/or the inhibitors of cellobiohydrolases for facile and useful enzymatic evaluation method (2012)
  • Author(s): 今場司朗
  • Organizer: 26th International Carbohydrate Symposium
  • Place of Presentation: マドリッド（スペイン）
• [Presentation] On‐chip synthesis strategy of glycan array (2012)
  • Author(s): 今場司朗
  • Organizer: Increasing the impact of glycoscience through new tools and technologies
  • Place of Presentation: サンセバスチャン（スペイン）
• [Presentation] 生物機能の解明と応用のための糖鎖自動合成技術および糖鎖ライブラリー合成技術の開発 (2012)
  • Author(s): 今場司朗
  • Organizer: 日本応用糖質科学会平成２４年度大会
  • Place of Presentation: 東京農工大学（東京都）
  • Invited
• [Presentation] 糖鎖チップの新時代 (2011)
  • Author(s): 今場司朗
  • Organizer: 日本応用糖質科学会平成23年度大会
  • Place of Presentation: 北海道
  • Year and Date: 2011-09-29
  • Invited
• [Presentation] 糖鎖チップの新時代 (2011)
  • Author(s): 今場司朗
  • Organizer: 日本応用糖質科学会平成23年度大会
  • Place of Presentation: 北海道大学(北海道)(招待講演)
  • Year and Date: 2011-09-29
• [Presentation] セロビオヒドロラーゼ活性評価法用基質である新規チオグリコシド類縁体の合成 (2011)
  • Author(s): 今場司朗、寺内毅、小山善幸、春見隆文
  • Organizer: 日本応用糖質科学会平成23年度大会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2011-09-28
• [Presentation] 新規チオオリゴ糖を用いたセロビオハイドロラーゼI及びIIの分別活性測定法の開発 (2011)
  • Author(s): 小山善幸、寺内毅、今場司朗、池正和、徳安健、荻原淳、加藤順、春見隆文
  • Organizer: 日本応用糖質科学会平成23年度大会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2011-09-28
• [Presentation] 酸化LDL受容体LOX-1のラフト局在におけるパルミトイル化の影響 (2011)
  • Author(s): 倉持(熊野)みゆき、謝秋宏、今場司朗、町田幸子
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2011-09-22
• [Presentation] A novel technique for constructing oligosaccharide microarrays (2011)
  • Author(s): 今場司朗、寺内毅、渡辺悟
  • Organizer: XXI International Symposium on Glycoconjugates
  • Place of Presentation: University of Vienna(オーストリア)
  • Year and Date: 2011-08-25
• [Presentation] フォトリソグラフィーを活用した糖鎖ナノデバイスの構築 (2011)
  • Author(s): 今場司朗
  • Organizer: 第51回澱粉科学懇談会
  • Place of Presentation: 兵衛向陽閣(兵庫)(招待講演)
  • Year and Date: 2011-06-03
• [Presentation] 糖脂質の新規精製法 (2010)
  • Author(s): 今場司朗
  • Organizer: 日本応用糖質科学会平成22年度大会
  • Place of Presentation: グランシップ(静岡県)
  • Year and Date: 2010-09-16
• [Presentation] A new method for cleavage of silicon-carbon linkers for clarifying the surface structure on glass plate of microarray (2010)
  • Author(s): 今場司朗
  • Organizer: 25th International Carbohydrate Symposium
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2010-08-02
• [Presentation] Purification study of commercial ganglioside GM1 (2010)
  • Author(s): 今場司朗
  • Organizer: Plant Polysaccharide and Applied Glycoscience Workshop 2010
  • Place of Presentation: 笹川ホール(東京都)
  • Year and Date: 2010-07-30
• [Presentation] 糖鎖チップの開発
  • Author(s): 今場司朗
  • Organizer: 第３６回日本分子生物学会
  • Place of Presentation: 神戸国際会議場（兵庫県）
• [Presentation] 糖鎖自動合成も可能にする全自動固相ライブラリー合成装置の開発
  • Author(s): 今場司朗
  • Organizer: つくば国際戦略特区産学官マッチングデー2013
  • Place of Presentation: つくば国際会議場（茨城県）
• [Presentation] 病気や毒素の診断薬・検査薬としてニーズが高い糖鎖チップの作製方法
  • Author(s): 今場司朗
  • Organizer: 第3回農研機構新技術説明会
  • Place of Presentation: JST東京別館ホール（東京都）
• [Book] 光学活性医薬品開発とキラルプロセス化学技術 (2011)
  • Author(s): 今場司朗
  • Publisher: サイエンス&テクノロジー