Single molecular analyses on effects of food ingredients on peptide absorption

• Project/Area Number: 22780131
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Food science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: KOBORI Toshiro 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・食品工学研究領域, 主任研究員 (10353971)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 食品吸収 / 原子間力顕微鏡 / 暗視野顕微鏡 / リポソーム / PepT1

Research Abstract

We optimized conditions for an in vitro experimental model where we could study interactions between PepT1, peptide transporter expressed on intestinal tract, and its substrate peptide. Purified PepT1 reconstituted on lipid bilayer andcantilever modified with a substrate peptide were prepared and applied to force measurement with atomic force microscopy. An interaction of Au-nanoparticle with Caco-2 monolayer could also be analyzed with dark-field microscopy, suggesting that the PepT1 interaction could be studied using live cells in combination with surface modification of Au with the substrate peptides.

Research Products

• [Journal Article] Gold Nanoparticles as Localization Markers for Direct and Live Imaging of Particle Absorption through a Caco-2 Cell Monolayer Using Dark-Field Microscopy (2012)
  • Author(s): Toshiro Kobori, Jun Watanabe, Hidenobu Nakao
  • Journal Title: Analytical Sciences Volume: 28 Issue: 1 Pages: 61
  • DOI: 10.2116/analsci.28.61
  • NAID: 130004827179
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed
• [Journal Article] 原子間力顕微鏡による食品の微細構造解析と相互作用評価 (2011)
  • Author(s): 小堀俊郎
  • Journal Title: 食糧 Volume: 49巻 Pages: 45-65
  • NAID: 40018848967
  • URL: http://www.naro.affrc.go.jp/org/nfri/publications/pdf/sousetsu/kanko_sou49/49_p045.pdf
  • Peer Reviewed
• [Presentation] Interactions between sodium caseinate and xanthan revealed by atomic force microscopy (2010)
  • Author(s): Toshiro Kobori, Atsuko Matsumoto, Shigeru Sugiyama
  • Organizer: International Conference on Food Applications of Nanoscale Science
  • Place of Presentation: 東京
  • Year and Date: 2010-06-11
• [Presentation] Interactions between sodium caseinate and xanthan revealed by atomic force microscopy (2010)
  • Author(s): Toshiro Kobori, Atsuko Matsumoto, Shigeru Sugiyama
  • Organizer: International Conference on Food Applications of Nanoscale Science
  • Place of Presentation: 東京ビックサイト
  • Year and Date: 2010-06-11
• [Presentation] The transient receptor potential channels TRPP2 and TRPC1 form a heterotetramer with a 2:2 stoichiometry and an alternating subunit arrangement (2010)
  • Author(s): Toshiro Kobori, Graham D. Smith, Richard Sandford, J. Michael Edwardson
  • Organizer: 第12回国際 SPM 会議
  • Place of Presentation: 札幌
  • Year and Date: 2010-05-10
• [Presentation] The transient receptor potential channels TRPP2 and TRPC1 form a heterotetramer with a 2:2 stoichiometry and an alternating subunit arrangement (2010)
  • Author(s): Toshiro Kobori, Graham D.Smith, Richard Sandford, J.Michael Edwardson
  • Organizer: 第12回国際SPM会議
  • Place of Presentation: 京王プラザホテル札幌
  • Year and Date: 2010-05-10
• [Book] Protocols for specimen and substrate preparation, and data correction methods, Atomic Force Microscopy in Nano Biology (2014)
  • Author(s): Toshiro Kobori, Kunio Takeyasu
  • Publisher: Pan StanfordPublishing Pte Ptd
• [Book] A Short Story of AFM in Biology, Atomic Force Microscopy in Nano Biology, Pan Stanford (2014)
  • Author(s): Aiko Hibino, Toshiro Kobori, Kunio Takeyasu
  • Publisher: Publishing Pte Ptd
• [Book] Protocols for specimen and substrate preparation, and data correction methods, Atomic Force Microscopy in Nano Biology (2014)
  • Author(s): Toshiro Kobori, Kunio Takeyasu
  • Total Pages: 400
  • Publisher: Pan Stanford Publishing Pte Ltd
• [Book] A Short Story of AFM in Biology, Atomic Force Microscopy in Nano Biology (2014)
  • Author(s): Aiko Hibino, Toshiro Kobori, Kunio Takeyasu
  • Total Pages: 400
  • Publisher: Pan Stanford Publishing Pte Ltd