Functional analysis of Foxo1 and PPRA. possibly involved in the species-specific lipid accumulation of fish
| Project/Area Number |
22780190
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Fisheries chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KANEKO Gen 東京大学, 大学院・農学生命科学研究科, 助教 (30466809)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 代謝・酵素 / 脂質蓄積 / PPARγ / Foxo1 / トラフグ / マダイ / 資質蓄積 / LPL / 転写因子 / リポタンパク質 / 細胞内局在 / プルダウンアッセイ / L6筋芽細胞 / IGF-I |
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| Research Abstract |
We cloned the full open reading frame of Foxo1 from torafugu. The transcripts were widely distributed in various tissues. Like the mammalian orthologues, torafugu Foxo1 protein was excluded from nucleus in a insulin-like growth factor I(IGF-I)-dependent manner, and bound to peroxisome proliferator-activated receptorγ(PPARγ) in vitro. On the other hand, the mRNA levels of PPARγand its putative target, lipoprotein lipase(LPL), showed a good correlation with the lipid content of liver and muscle in torafugu and red seabream. These results suggest that PPARγpromotes the species-specific lipid accumulation in fish, and Foxo1 functions as an IGF-dependent inhibitor of PPARγ.
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Report
(3 results)
Research Products
(27 results)
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[Journal Article] Correlation with larval body size of mRNA levels of growth hormone, growth hormone receptor I and insulin-like growth factor I in larval torafugu Takifugu rubripes.2011
Author(s)
Kaneko G^*, Furukawa S^*, Kurosu Y, Yamada T, Takeshima H, Nishida M, Mitsuboshi T, Otaka T, Shirasu K, Koda T, Takemasa Y, Aki S, Mochizuki T, Fukushima H, Fukuda Y, Kinoshita S, Asakawa S, Watabe S
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Journal Title
J. Fish Biol
Volume: 79
Pages: 854-874
Related Report
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[Journal Article] Correlation with larval body size of mRNA levels of growth hormone, growth hormone receptor I and insulin-like growth factor I in larval torafugu Takifugu rubripes2011
Author(s)
Kaneko G, Furukawa S, Kurosu Y, Yamada T, Takeshima H, Nishida M, Mitsuboshi T, Otaka T, Shirasu K, Koda T, Takemasa Y, Aki S, Mochizuki T, Fukushima H, Fukuda Y, Kinoshita S, Asakawa S, Watabe S
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Journal Title
J. Fish Biol.
Volume: 79
Issue: 4
Pages: 854-874
DOI
Related Report
Peer Reviewed
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