| Project/Area Number |
22780190
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Fisheries chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KANEKO Gen 東京大学, 大学院・農学生命科学研究科, 助教 (30466809)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 代謝・酵素 / 脂質蓄積 / PPARγ / Foxo1 / トラフグ / マダイ / 資質蓄積 / LPL / 転写因子 / リポタンパク質 / 細胞内局在 / プルダウンアッセイ / L6筋芽細胞 / IGF-I |
|---|
| Research Abstract |
We cloned the full open reading frame of Foxo1 from torafugu. The transcripts were widely distributed in various tissues. Like the mammalian orthologues, torafugu Foxo1 protein was excluded from nucleus in a insulin-like growth factor I(IGF-I)-dependent manner, and bound to peroxisome proliferator-activated receptorγ(PPARγ) in vitro. On the other hand, the mRNA levels of PPARγand its putative target, lipoprotein lipase(LPL), showed a good correlation with the lipid content of liver and muscle in torafugu and red seabream. These results suggest that PPARγpromotes the species-specific lipid accumulation in fish, and Foxo1 functions as an IGF-dependent inhibitor of PPARγ.
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